1887

Abstract

SUMMARY

Staphylococci grow and cause infection under the iron-restricted conditions found in vivo. They therefore must possess mechanisms to obtain iron for metabolism from this environment. To determine if staphylococci can extract iron bound to human transferrin, we labelled transferrin with Fe and performed uptake assays on cells grown in iron-restricted and iron-plentiful conditions. Growing cultures of Staphylococcus aureus NCTC 8532 could take up radioactive iron during mid- to late-exponential phase of growth. This process was iron-regulated and did not require direct contact between the cell and the labelled transferrin. Siderophore production was detected during this phase, but reductase or protease activity was not. S. epidermidis ATCC 14990 could not access Fe bound to transferrin, nor did this isolate produce siderophore, reductase or protease. This difference in the ability to acquire iron bound to transferrin may contribute to the increased virulence of S. aureus when compared to S. epidermidis.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-141-1-197
1995-01-01
2021-08-02
Loading full text...

Full text loading...

/deliver/fulltext/micro/141/1/mic-141-1-197.html?itemId=/content/journal/micro/10.1099/00221287-141-1-197&mimeType=html&fmt=ahah

References

  1. Brock J. H., Ng J. 1993; The effect of desferrioxamine on the growth of Staphylococcus aureus, Yersinia enterocolitica and Streptococcus faecalis in human serum: uptake of desferrioxamine-bound iron. FEMS Microbiol Lett 20:439–442
    [Google Scholar]
  2. Brock J. H., Williams P. H., Licéaga J., Wooldridge K. G. 1991; Relative availability of transferrin-bound iron and cell-derived iron to aerobactin-producing and enterochelin-producing strains of Escherichia coli and to other microorganisms. Infect Immun 59:3185–3190
    [Google Scholar]
  3. Courcol R. J., Lambert P. A., Fournier P., Martin G. R., Brown M. R. W. 1991; Effects of iron depletion and sub-inhibitory concentrations of antibiotics on siderophore production by Staphylococcus aureus . J Antimicrob Chemother 28:663–668
    [Google Scholar]
  4. Cowart R. E., Foster B. G. 1985; Differential effects of iron on the growth of Listeria monocytogenes: minimum requirements and mechanism of acquisition. J Inject Dis 151:721–730
    [Google Scholar]
  5. Cowart R. E., Marquardt M. P., Foster B. G. 1980; The removal of iron and other trace elements from a complex bacteriological medium. Microbios Lett 13:117–122
    [Google Scholar]
  6. Drechsel H., Freund S., Nicholson G., Haag H., Jung O., Zähner H., Jung G. 1993; Purification and chemical characterization of staphyloferrin B, a hydrophilic siderophore from staphylococci. BioMetals 6185–192
    [Google Scholar]
  7. Francis R. T. Jr, Booth J. W., Becker R. R. 1985; Uptake of iron from hemoglobin and the haptoglobin-hemoglobin complex by hemolytic bacteria. Int J Biochem 17:767–773
    [Google Scholar]
  8. Lindsay J. A., Riley T. V. 1994; Staphylococcal iron requirements, siderophore production and iron-regulated protein expression. Infect Immun 62:2309–2314
    [Google Scholar]
  9. Marcelis J. H., Den Daas-Slagt H. J., Hoogkamp-Korstanje J. A. A. 1978; Iron requirements and chelator production of staphylococci, Streptococcus faecalis and enterobacteriaceae. Antonie Leeuwenhoek 44:257–267
    [Google Scholar]
  10. Meiwes J., Fiedler H.-P., Haag H., Zähner H., Konetschny-Rapp S., Jung G. 1990; Isolation and characterization of staphyloferrin A, a compound with siderophore activity from Staphylococcus hyicus DSM 20459. FEMS Microbiol Lett 67:201–206
    [Google Scholar]
  11. Modun B., Kendall D., Williams P. 1994; Staphylococci express a receptor for human transferrin: identification of a 42-kilodalton cell wall transferrin-binding protein. Infect Immun 62:3850–3858
    [Google Scholar]
  12. Naidu A. S., Miedzobrodzki J., Musser J. M., Rosdahl V. T., Hedström S.-Å., Forsgren A. 1991; Human lactoferrin binding in clinical isolates of Staphylococcus aureus . J Med Microbiol 34:323–328
    [Google Scholar]
  13. Naidu A. S., Andersson M., Forsgren A. 1992; Identification of a human lactoferrin-binding protein in Staphylococcus aureus . J Med Microbiol 36:177–183
    [Google Scholar]
  14. Neuhoff V., Arold N., Taube D., Ehrhardt W. 1988; Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250. Electrophoresis 9255–262
    [Google Scholar]
  15. Pfaller M. A., Herwaldt L. A. 1988; Laboratory, clinical and epidemiological aspects of coagulase-negative staphylococci. Clin Microbiol Rev 1:281–299
    [Google Scholar]
  16. Schade A. L. 1963; Significance of serum iron for the growth, biological characteristics, and metabolism of Staphylococcus aureus . Biochemische Zeitschrift 338:140–148
    [Google Scholar]
  17. Schwyn B., Neilands J. B. 1987; Universal chemical assay for the detection and determination of siderophores. Anal Biochem 160:47–56
    [Google Scholar]
  18. Williams P., Griffiths E. 1992; Bacterial transferrin receptors - structure, function and contribution to virulence. Med Microbiol Immunol 181:301–322
    [Google Scholar]
  19. Wooldridge K. G., Williams P. H. 1993; Iron uptake mechanisms of pathogenic bacteria. FEMS Microbiol Rev 12:325–348
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-141-1-197
Loading
/content/journal/micro/10.1099/00221287-141-1-197
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error