1887

Abstract

SUMMARY: In most plasmids which have been studied to date the functions required for plasmid replication are clustered in a 2—3 kb region. However, in all known naturally occurring plasmids of the incompatibility group P the essential replication functions, the vegetative replication origin and which encodes proteins essential to activate are separated by blocks of DNA consisting of either known genes conferring resistance to antimicrobial agents and/or putative transposable elements. Nucleotide sequence comparisons reported here reveal that these blocks of DNA have inserted at different points into a backbone of DNA common to IncP plasmids. The results indicate that in the common ancestor of present IncP plasmids and must have been contiguous, whilst a ρ-independent transcriptional terminator, now lost in IncPα plasmids, may have prevented operon transcription from interfering with the activity of

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1993-08-01
2021-10-23
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References

  1. Ayres E., Saadi S., Schreiner H.C., Thomson V.J., Figurski D.H. 1991; Differentiation of lethal and non-lethal, kor-regulated functions in thekilBregionofbroad host-range plasmid RK2.. Plasmid 25:53–63
    [Google Scholar]
  2. Birnboim H.C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA.. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  3. Bolivar F., Rodriguez R.L., Green P.J., Betlach M.V., Heynecker H.L., Boyer H.W., Crosa J.W., Falkow S. 1977; Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.. Gene 2:95–113
    [Google Scholar]
  4. Chikami G.K., Guiney D.G., Schmidhauser T.J., Helinski D.R. 1985; Comparison of ten IncP plasmids: homology in the regionsinvolved in plasmid replication.. Journal of Bacteriology 162:656–660
    [Google Scholar]
  5. Couturier M., Bex F., Bergquist P.L., Maas W.K. 1988; Identification and classification of bacterial plasmids.. Microbiological Reviews 52:375–395
    [Google Scholar]
  6. Cross M.A., Warne S.R., Thomas C.M. 1986; Analysis of the vegetative replication origin of broad host range plasmid RK2 by transposon mutagenesis.. Plasmid 15:132–146
    [Google Scholar]
  7. Currier T.C., Morgan M.K. 1981; Restriction endonuclease analyses of the incompatibilitygroup P-1 plasmids RK2, RP1, RP4, R68 and R68.45.. Current Microbiology 5:323–327
    [Google Scholar]
  8. De La Cruz F., Grinsted J. 1982; Genetic and molecular characterization of Tn21, a multiple resistance transposon from R100.1.. Journal of Bacteriology 151:222–228
    [Google Scholar]
  9. Devereux J.P., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX.. Nucleic Acids Research 12:387–395
    [Google Scholar]
  10. Figurski D.H., Pohlman R.F., Bechhofer D.H., Prince A.S., Kelton C.A. 1982; The broad-host-range plasmid RK2 encodes multiplekilgenes potentially lethal toEscherichia colihost cells.. Proceedings of the National Academy of Sciences of the United States of America 79:1935–1939
    [Google Scholar]
  11. Grinsted J.E., Brown N.L. 1984; A Tn21 terminal sequence withinTn501: complementation oftnpAgene function and transposon evolution.. Molecular and General Genetics 197:497–502
    [Google Scholar]
  12. Grinsted J., De La Cruz F., Altenbuchner J., Schmitt R. 1982; Complementation analysis of transposition oftnpAmutants of Tn3, Tn21, Tn501 and Tn1721.. Plasmid 8:276–286
    [Google Scholar]
  13. Hedges R.W., Matthew M., Smith D.I., Cresswell J.M., Jacob A.E. 1977; Properties of a transposon conferring resistance to penicillins and streptomycin.. Gene 1:241–253
    [Google Scholar]
  14. Jobanputra R.S., Datta N. 1974; Trimethoprim R-factors in Enterobacteria from clinical specimens.. Journal of Medical Micro-biology 7:169–177
    [Google Scholar]
  15. Kahn M., Kolter R., Thomas C., Figurski D., Meyer R., Remaut E., Helinski D.R. 1979; Plasmid cloning vehicles derived from plasmids ColEl, F,R6K and RK2.. Methods in Enzymology 68:268–280
    [Google Scholar]
  16. Kratz J., Schmidt F., Wiedemann B. 1983; Characterization of Tn2411 and Tn2410,two transposons derived from R-plasmid R1767 and related to Tn2603and Tn21.. Journal of Bacteriology 155:1333–1342
    [Google Scholar]
  17. Lanka E., FÜRste J.P., Yakobson E., Guiney D.G. 1985; Conserved regions at the DNA primase locus of the IncPɑ and IncPβ plasmids.. Plasmid 14:217–223
    [Google Scholar]
  18. Maniatis T., Fritsch E.F., Sambrook J. 1982 Molecular cloning: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.;
    [Google Scholar]
  19. Maxam A., Gilbert W. 1980; Sequencing end-labelled DNA with base-specific cleavage.. Methods in Enzymology 65:449–560
    [Google Scholar]
  20. Norrander J., Kempe T., Messing J. 1983; Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis.. Gene 27:101–106
    [Google Scholar]
  21. Perri S., Helinski D.R., Toukdarian A. 1991; Interactions of plasmid-encoded replication initiation proteins with the origin of DNA replication in the broad host range plasmid RK2.. Journal of Biological Chemistry 266:12536–12543
    [Google Scholar]
  22. Pinkney M., Diaz R., Lanka E., Thomas C.M. 1988; Replication of mini RK2 plasmid in extracts ofEscherichia colirequires plasmid-encoded protein TrfA and host-encoded proteins DnaA, B, G, DNA gyrase and DNA polymerase III.. Journal of Molecular Biology 204:927–938
    [Google Scholar]
  23. Sanger F., Nicklen S., Coulson A.R. 1977; DNA sequencing with chain-terminating inhibitors.. Proceedings of the National Academy of Sciences of the United States of America 74:5463–5467
    [Google Scholar]
  24. Shingler V., Thomas C.M. 1984; Analysis of thetrfAregion of broad hostrange plasmid RK2 by transposon mutagenesis and identification of polypeptide products.. Journal of Molecular Biology 175:229–250
    [Google Scholar]
  25. Smith C.A., Thomas C.M. 1984; Nucleotide sequence of thetrfAgene of the broad host range plasmid RK2.. Journal of Molecular Biology 175:251–262
    [Google Scholar]
  26. Smith C.A., Thomas C.M. 1985; Comparison of the nucleotide sequences of the vegetativereplication origins of broad host range IncP plasmids R751 and RK2 reveals conserved features of probable functional importance.. Nucleic Acids Research 13:557–572
    [Google Scholar]
  27. Smith C.A., Thomas C.M. 1987; Comparison of the organization of the genomes of phenotypically diverse plasmids of incompatibility group P: members of the IncP#x03B2; subgroup are closely related.. Molecular and General Genetics 206:419–427
    [Google Scholar]
  28. Smith C.A., Thomas C.M. 1989; Relationships and evolution of IncP plasmids.. In Promiscuous Plasmids of Gram-Negative Bacteria pp. 57–77 Thomas C.M. Edited by London: Academic Press;
    [Google Scholar]
  29. Stalker D.M., Thomas C.M., Helinski D.R. 1981; Nucleotide sequence of the origin of replication of the broad host range plasmid RK2.. Molecular and General Genetics 181:8–12
    [Google Scholar]
  30. Thomas C.M., Smith C.A. 1987; Incompatibility group P plasmids: genetics, evolution and use in genetic manipulation.. Annual Review of Microbiology 41:77–101
    [Google Scholar]
  31. Thomas C.M., Cross M.A., Hussain A.A.K., Smith C.A. 1984; Analysis of the copy number control elements in the region of the vegetative replication origin of broad host range plasmid RK2.. EM BO Journal 3:57–63
    [Google Scholar]
  32. Thomas C.M., Meyer R., Helinski D.R. 1980; Regions of the broad host range plasmid RK2 which are essential for replication and maintenance.. Journal of Bacteriology 141:213–222
    [Google Scholar]
  33. Vieira J., Messing J. 1982; The pUC plasmids, an Ml3 mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.. Gene 19:259–268
    [Google Scholar]
  34. Villaroel R., Hedges R.W., Maenhaut R., Leemans J., Engler G., Van Montagu M., Schell J. 1983; Heteroduplex analysis of P-plasmid evolution: the role of insertion and deletion of transposable elements.. Molecular and General Genetics 189:390–399
    [Google Scholar]
  35. Waters S.H., Grinsted J.E., Rogowsky P., Altenbuchner J., Schmitt R. 1983; The tetracycline resistance determinants of RP1 and Tn1721:nucleotide sequence analysis.. Nucleic Acids Research 11:6089–6105
    [Google Scholar]
  36. Womble D.D., Rownd R.H. 1988; Genetic and physical map of plasmid NR1: comparison withother IncFII antibiotic resistance plasmids.. Microbiological Reviews 52:433–451
    [Google Scholar]
  37. Yakobson E., Guiney D. 1983; Homology in the transfer origins of broad-host-range IncP plasmids: definition of two subgroups of P-plasmids.. Molecular and General Genetics 192:436–438
    [Google Scholar]
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