1887

Abstract

Summary: The DNA sequence of the gene encoding the early and specific immunogenic protein P36 of has been determined. Comparison of the DNA sequence and the deduced amino acid sequence of P36 with known genes and proteins in data banks indicated that P36 is a L-lactate dehydrogenase (LDH) (EC 1.1.1.27). Biochemical analysis of protein P36 expressed from the cloned gene in confirmed that P36 has L-lactate dehydrogenase activity. Protein P36 of therefore is termed LDH and its gene LDH was shown to contain the typical domains of LDH of other bacterial species. Immunologically however, we have shown that polyclonal antibodies against LDH do not cross-react with related LDH and show high specificity for . The gene is preceded by several typical −10 sequences found in promoters of prokaryotes, but lacks the −35 sequence. Sequences rich in A + T, however, precede the −10 boxes, suggesting that factors involved in transcription initiation and their regulation may be different in compared to other bacterial species, but the putative ribosome binding site seems to be conserved.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-139-2-317
1993-02-01
2019-11-22
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-2-317
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error