1887

Abstract

A 1.8 kb III DNA fragment containing the gene of alkalophilic sp. C125 has been cloned into plasmid pUC119 using the gene as a probe. The complete nucleotide sequence of the cloned DNA indicated that it contained one complete ORF and parts of two other ORFs. The similarity of these ORFs to the sequences of the proteins indicated that they were the genes for ribosomal protein L15–SecY–adenylate kinase, in that order. The gene product of the alkalophilic sp. C125 homologue was composed of 431 amino acids and its value has been calculated to be 47100. The distribution of hydrophobic amino acids in the gene product suggested that the protein was a membrane integrated protein with ten transmembrane segments. The total amino acid sequence of alkalophilic sp. C125 homologue showed 69.7% homology with that of . Regions of remarkably high homology (78% identity) were present in transmembrane regions, and cytoplasmic domains (73% identity) with less homologous regions present in extracellular domains (43% identity).

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-138-7-1365
1992-07-01
2021-04-21
Loading full text...

Full text loading...

/deliver/fulltext/micro/138/7/mic-138-7-1365.html?itemId=/content/journal/micro/10.1099/00221287-138-7-1365&mimeType=html&fmt=ahah

References

  1. Akiyama Y., Ito K. 1987; Topology analysis of the secY protein, an integral membrane protein involved in protein export in Escherichia coli. EMBO Journal 6 3465 3470
    [Google Scholar]
  2. Bankatis V., Bassford P. 1985; Proper interaction between at least two components is required for efficient export of proteins to the Escherichia coli cell envelope. Journal of Bacteriology 161 169 178
    [Google Scholar]
  3. Emr S., Hanley-Way S., Shilhavy T. 1981; Suppressor mutations that restore export of a protein with a defective signal sequence. Cell 23 79 88
    [Google Scholar]
  4. Henikoff S. 1984; Undirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene 28 351 359
    [Google Scholar]
  5. Horikoshi K. 1991 Microorganisms in the Alkaline Environment Tokyo: Kodansha;
    [Google Scholar]
  6. Ito K., Wittekind M., Nomura M., Shiba K., Yura T., Miura A., Nashimoto H. 1983; A temperature-sensitive mutant of E. coli. exhibiting slow processing of exported proteins. Cell 32 789 797
    [Google Scholar]
  7. Kudo T., Hino M., Kitada M., Horikoshi K. 1990; DNA sequences required for the alkalophily of Bacillus sp. strain C125 are located close together on its chromosomal DNA. Journal of Bacteriology 172 7282 7283
    [Google Scholar]
  8. Kyte J., Doolittle R. F. 1982; A simple method for displaying the hydropathic character of a protein. Journal of Molecular Biology 157 105 132
    [Google Scholar]
  9. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  10. Michaelis S., Beckwith J. 1982; Mechanism of incorporation of cell envelope proteins in Escherichia coli. Annual Review of Microbiology 36 435 465
    [Google Scholar]
  11. Nakamura K., Nakamura A., Takamatsu H., Yoshikawa H., Yamane K. 1990; Cloning and characterization of a Bacillus subtilis gene homologous to E. coli secY. Journal of Biochemistry 107 603 607
    [Google Scholar]
  12. Sanger F., Nicklen S., Coulson A. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences of the United States of America 74 5463 5467
    [Google Scholar]
  13. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98 503 517
    [Google Scholar]
  14. Vieira J., Messing J. 1987; Production of single-stranded plasmid DNA. Methods in Enzymology 153 3 11
    [Google Scholar]
  15. Yoshikawa H., Doi R. 1990; Sequence of the Bacillus subtilis spectinomycin resistance gene region. Nucleic Acids Research 18 1647
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-138-7-1365
Loading
/content/journal/micro/10.1099/00221287-138-7-1365
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error