1887

Abstract

Sexual and asexual differentiation of are influenced by nitrogen availability and blue light. Nitrogen limitation induces the production of protoperithecia on solid medium and conidia in liquid medium. Both developmental processes are stimulated by blue light. We have analysed changes in mRNA levels for a variety of light-inducible genes ( and ) under conditions of nitrogen limitation. We show that the photoregulated genes and are also regulated by nitrogen limitation. These genes exhibited nitrogen regulation in the nonphotoresponsive mutant strains and . Therefore, the and gene products, although necessary for photoregulation of these and genes, are not required for nitrogen regulation.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-138-10-2045
1992-10-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/138/10/mic-138-10-2045.html?itemId=/content/journal/micro/10.1099/00221287-138-10-2045&mimeType=html&fmt=ahah

References

  1. Berlin V., & Yanofsky C. 1985; Isolation and characterization of genes differentially expressed during conidiation of Neurospora crassa . Molecular and Cellular Biology 5:849–855
    [Google Scholar]
  2. Boll W., , Fujisawa J.-I., , Niemi J., & Weismann C. 1986; A new approach to high sensitivity differential hybridization. Gene 50:41–53
    [Google Scholar]
  3. Chambers J. A. A., , Hinkelammert K., & Russo V. E. A. 1985; Light-regulated protein and poly(A)+ mRNA synthesis in Neurospora crassa . EMBO Journal 4:3649–3653
    [Google Scholar]
  4. Charles H. P. 1962; Response of Neurospora mutants to carbon dioxide. Nature, London 195:359–360
    [Google Scholar]
  5. Dantzig A.H., , Wiegmann JR, , F-L., & Nason A. 1979; Regulation of the glutamate dehydrogenase in nit-2 and am mutants of Neurospora crassa . Journal of Bacteriology 137:1333–1339
    [Google Scholar]
  6. Degli-Innocenti F., , Pohl U., & Russo V. E. A. 1983; Photoinduction of protoperithecia in Neurospora crassa by blue light. Photochemistry and Photobiology 37:49–51
    [Google Scholar]
  7. Degli-Innocenti F., & Russo V. E. A. 1984a; Isolation ofnew white collar mutants of Neurospora crassa and studies on their behavior in the blue-light-induced formation of protoperithecia. Journal of Bacteriology 159:757–761
    [Google Scholar]
  8. Degli-Innocenti F., & Russo V. E. A. 1984b Genetic analysis of blue-light-induced responces in Neurospora crassa. . In Blue Light Effects in Biological Systems, pp. 214–219 Edited by Senger H. Berlin: Springer Verlag;
    [Google Scholar]
  9. Faull A. F. 1930; On the resistance of Neurospora crassa . Mycologia 22:288–303
    [Google Scholar]
  10. Feinberg A. P., & Vogelstein B. 1983; A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Analytical Biochemistry 132:6–13
    [Google Scholar]
  11. Frederick G.D., & Kinsey J. A. 1990; Distant upstream regulatory sequences control the level of expression of the am (GDH) locus of Neurospora crassa . Current Genetics 18:53–58
    [Google Scholar]
  12. Guignard R., , Grange F., & Turian G. 1984; Microcycle conidiation induced by partial nitrogen deprivation in Neurospora crassa . Canadian Journal of Microbiology 30:1210–1215
    [Google Scholar]
  13. Harding R. W., & Melles S. 1984; Genetic analysis of the phototropism of Neurospora crassa perithecial beaks using white collar and albino mutants. Plant Physiology 72:996–1000
    [Google Scholar]
  14. Harding R. W., & Turner R. V. 1981; Photoregulation of the carotenoid biosynthetic pathway in albino and white collar mutants of Neurospora crassa . Plant Physiology 68:745–749
    [Google Scholar]
  15. Kinnaird J. H., , Keighren M. A., , Kinsey J. A., , Eaton M., & Fincham J. R. S. 1982; Cloning of the am (glutamate dehydrogen-ase) gene of Neurospora crassa through the use of a synthetic DNA probe. Gene 20:387–396
    [Google Scholar]
  16. Lauter F.-R., & Russo V. E. A. 1990; Light-induced dephosphorylation of a 33 kDa protein in the wild-type strain of Neurospora crassa: the regulatory mutants we-1 and wc-2 are abnormal. Journal of Photochemistry and Photobiology 5:95–103
    [Google Scholar]
  17. Lauter F.-R., & Russo V. E. A. 1991; Blue light induction of conidiation-specific genes in Neurospora crassa . Nucleic Acids Research 19:6883–6886
    [Google Scholar]
  18. Müller B., & Russo V. E. A. 1989; Nitrogen starvation or glucose limitation induces conidiation in constantly shaken liquid cultures of Neurospora crassa . Fungal Genetics Newsletters 36:58–60
    [Google Scholar]
  19. Nawrath C., & Russo V. E. A. 1990; Fast induction of translatable mRNA by blue light in Neurospora crassa wildtype: the wc-1 and wc-2 mutants are blind. Journal of Photochemistry and Photobiology 4:261–271
    [Google Scholar]
  20. Nelson M.A., , Morelli G., , Carattoli A., , Romano N., & Macino G. 1989; Molecular cloning of a Neurospora crassa carotenoid biosynthetic gene (albino-3) regulated by blue light and the products of the white collar genes. Molecular and Cellular Biology 9:1271–1276
    [Google Scholar]
  21. Nelson R. E., , Selitrennikoff C. P., & Siegel R. W. 1975; Results and Problems in Cell Differentiation. pp. 291–310 Edited by Reinert J. and Holzer H. Berlin: Springer;
    [Google Scholar]
  22. Orbach M. J., , Porro E. B., and Yanofsky C. 1986; Cloning and characterization of the gene for P-tubulin from a benomyl-resistant mutant of Neurospora crassa and its use as a dominant selectable marker. Molecular and Cellular Biology 6:2452–2461
    [Google Scholar]
  23. Ricci M., , Krappmann D., & Russo V. E. A. 1991; Nitrogen and carbon starvation regulate conidiation and protoperithecia forma-tion in Neurospora crassa grown on solid medium. Fungal Genetics Newsletters 38:87–88
    [Google Scholar]
  24. Roberts A. N., , Berlin V., , Hager K., & Yanofsky C. 1988; Molecular analysis of a Neurospora crassa gene expressed during conidiation. Molecular and Cellular Biology 8:2411–2418
    [Google Scholar]
  25. Russo V. E. A. 1986; Are carotinoids the blue light photoreceptor in the photoinduction of protoperithecia in Neurospora crassa? . Planta 168:56–60
    [Google Scholar]
  26. Russo V. E. A. 1988; Blue light induces circadian rhythms in the bd mutant of Neurospora: double mutants bd,wc-I and bd,wc-2 are blind. Journal of Photochemistry and Photobiology 2:59–65
    [Google Scholar]
  27. Russo V. E. A., , POHL U., & VOLPI L. 1981; Carbon dioxide inhibits photogenesis in Phycomyces and blue light overcomes this inhibition. Photochemistry and Photobiology 34:233–236
    [Google Scholar]
  28. Sachs M. S., & Yanofsky C. 1991; Developmental expression of genes involved in conidiation and aminoacid starvation in Neurospora crassa . Developmental Biology 148:117–128
    [Google Scholar]
  29. Sargent M. L., & Briggs W. R. 1967; The effects of light on a circadian rhythm of conidiation in Neurospora . Plant Physiology 42:1504–1510
    [Google Scholar]
  30. Sargent M. L., & Kaldenborn. 1972; Effects of medium composition and carbon dioxide on circadian conidiation in Neurospora . Plant Physiology 50:171–175
    [Google Scholar]
  31. Sargent M. L., , Briggs W. R., & Woodward D. O. 1966; Circadian nature of a rhythm expressed by an invertaseless strain of Neurospora crassa . Plant Physiology 41:1343–1349
    [Google Scholar]
  32. Schmidhauser T. J., , Lauter F.-R., , Russo V. E. A., & Yanofsky C. 1990; Cloning, sequence, and photoregulation of al-I, a carotenoid biosynthetic gene of Neurospora crassa . Molecular and Cellular Biology 10:5064–5070
    [Google Scholar]
  33. Siegel R. W., , Matsuyama S. S., & Urey J. C. 1968; Induced macroconidiation formation in Neurospora crassa . Experientia 24:1179–1181
    [Google Scholar]
  34. Sokolovsky V. Y., , Kaldenhoff R., , Ricci M., & Russo V. E. A. 1990; Fast and reliable mini-prep RNA extraction from Neurospora crassa . Fungal Genetics Newsletters 37:39–40
    [Google Scholar]
  35. Sommer T., , Innocenti Degli, , F., & Russo V. E. A. 1987; Role of nitrogen in the photoinduction of protoperithecia and carotenoids in Neurospora crassa . Planta 170:205–208
    [Google Scholar]
  36. Sommer T., , Chambers J. A. A., , Eberle J., , Lauter F.-R., & Russo V. E. A. 1989; Fast light-regulated genes Neurospora crassa . Nucleic Acids Research 17:5713–5723
    [Google Scholar]
  37. Springer M. L., & Yanofsky C. 1989; A morphological and genetic analysis of the conidiophore development in Neurospora crassa . Genes and Development 3:559–571
    [Google Scholar]
  38. Turian G. 1977 Fungal differentiation. In Biotechnology and Fungal Differentiation, pp. 1–15 Edited by Meyrath J., & person-group-type="editor"> Bu'Lock J. D. London: Academic Press;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-138-10-2045
Loading
/content/journal/micro/10.1099/00221287-138-10-2045
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error