1887

Abstract

It was established that grew rapidly in a simple medium containing yeast extract (0·2 %, w/v) plus glucose (2 %, w/v). These cultures were in or near to a state of nitrogen limitation and the concentration of secreted aspartate proteinase increased rapidly (within 3–4 h) on addition of BSA. Synthesis and secretion were apparently controlled both positively (induction by albumin or, more probably, the peptides produced from it) and negatively (repression by NHCl). A small intracellular pool of the enzyme was detected during production of the enzyme and this pool decreased with the cessation of synthesis and secretion. A stable mutant, IR24, was isolated which secreted less than 0·3% of the amount of the proteinase exported by the parent strain ATCC 10261. The LD values for mutant IR24 and the parent strain administered intravenously to mice were > 1·0 × 10 and 1·6 × 10 c.f.u. kg respectively.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-136-4-687
1990-04-01
2021-08-04
Loading full text...

Full text loading...

/deliver/fulltext/micro/136/4/mic-136-4-687.html?itemId=/content/journal/micro/10.1099/00221287-136-4-687&mimeType=html&fmt=ahah

References

  1. Achstetter T., Wolf D.H. 1985; Proteinases, proteolysis and biological control in the yeast Saccharomyces cerevisiae. Yeast 1:139–157
    [Google Scholar]
  2. Bistoni F., Vecchiarelli A., Cenci A., Puccetti P., Marconi P., Cassone A. 1986; Evidence for macrophage mediated protection against lethal Candida albicans infection. Infection and Immunity 51:668–674
    [Google Scholar]
  3. Cassone A., De Bernardis F., Mondello F., Ceddia T., Agatensi L. 1987; Evidence for a correlation between proteinase secretion and vulvovaginal candidosis. Journal of Infectious Diseases 156:777–783
    [Google Scholar]
  4. Cohen B.L. 1981; Regulation of proteinase production in Aspergillus. Transactions of the British Mycological Society 76:447–450
    [Google Scholar]
  5. Cohen B.L., Drucker H. 1977; Regulation of exocellular protease in Neurospora crassa: induction and repression under conditions of nitrogen starvation. Archives of Biochemistry and Biophysics 182:601–613
    [Google Scholar]
  6. Crandall M., Edwards J.E. 1987; Segregation of proteinasenegative mutants from heterozygous Candida albicans. Journal of General Microbiology 133:2817–2824
    [Google Scholar]
  7. Drucker H. 1973; Regulation of exocellular proteases in Neurosporacrassa: role of Neurospora proteases in induction. Journal of Bacteriology 116:593–599
    [Google Scholar]
  8. Germaine G.R., Tellefson L.M., Johnson G.I. 1978; Proteolytic activity of Candida albicans: action on human salivary proteins. Infection and Immunity 22:861–866
    [Google Scholar]
  9. Ghannoum M., Abu Elteen K. 1986; Correlative relationship between proteinase production, adherence and pathogenicity of various strains of Candida albicans. Journal of Medical and Veterinary Mycology 24:407–413
    [Google Scholar]
  10. Jenthoff N., Dearborn D.G. 1979; Labelling of proteins by reductive methylation using sodium cyanoborohydride. Journal of Biological Chemistry 254:4359–4365
    [Google Scholar]
  11. Kwon-Chung K.J., Lehman D., Good C., Magee P.T. 1985; Genetic evidence for role of extracellular proteinase in virulence of Candida albicans. Infection and Immunity 49:571–575
    [Google Scholar]
  12. Laemmli U.K., Favre M. 1973; Phage T4 head assembly. Journal of Molecular Biology 80:575–599
    [Google Scholar]
  13. Lasure L.L. 1980; Regulation of extracellular acid protease in Mucormiehei. Mycologia 72:483–492
    [Google Scholar]
  14. Macdonald F. 1984; Secretion of inducible proteinase by pathogenic Candida species. Journal of Medical and Veterinary Mycology 22:79–82
    [Google Scholar]
  15. Macdonald F., Odds F.C. 1980; Inducible proteinase of Candida albicans in diagnostic serology and the pathogenesis of systemic candidosis. Journal of Medical Microbiology 13:423–435
    [Google Scholar]
  16. Macdonald F., Odds F.C. 1983; Virulence for mice of a proteinase secreting strain of Candida albicans and a proteinase-deficient mutant. Journal of General Microbiology 129:431–438
    [Google Scholar]
  17. Matsudaira P.T., Burgess D.R. 1978; SDS microslab linear gradient polyacrylamide gel electrophoresis. Analytical Biochemistry 87:386–396
    [Google Scholar]
  18. Meyer S.A., Ahearn D.G., Yarrow D. 1984 In The Yeasts: a Taxonomic Study, pp 585–844 Kreger van Rij N. S. A. Edited by Amsterdam: Elsevier Science Publishers;
    [Google Scholar]
  19. North M.J. 1982; Comparative biochemistry of the proteinases of eucaryotic microorganisms. Microbiological Reviews 46:308–340
    [Google Scholar]
  20. Odds F.C. 1988 Candida and Candidosis; a Review and Bibliography, 2nd edn.. pp 259–267 London: Balliere Tindall;
    [Google Scholar]
  21. Peterson G.L. 1977; A simplification of the protein assay method of Lowry et al. which is more generally acceptable. Analytical Biochemistry 83:346–356
    [Google Scholar]
  22. Poulter R., Jeffery K., Hubbard M.J., Shepherd M.G., Sullivan P.A. 1981; Parasexual genetic analysis of Candida albicans by spheroplast fusion. Journal of Bacteriology 146:833–840
    [Google Scholar]
  23. Rembold H., Fasold H., Staib F. 1968; Purification and characterisation of a proteolytic activity from Candida albicans. Biochimica et Biophysica Acta 167:399–406
    [Google Scholar]
  24. Rothman J.E., Hunter C.P., Valls L.A., Stevens T.H. 1986; Overproduction-induced mislocation of a yeast vacuolar protein allows isolation of its structural gene. Proceedings of the National Academy of Sciences of the United States of America 83:3248–3252
    [Google Scholar]
  25. Ruchel R. 1981; Properties of a purified proteinase from the yeast Candida albicans. Biochimica et Biophysica Acta 659:99–113
    [Google Scholar]
  26. Ruchel R. 1983; On the role of proteinases from Candida albicans in the pathogenesis of acronecrosis. Zentralblatt für Bakteriologie,Mikrobiologie und Hygiene (Abteilung I) 255:524–537
    [Google Scholar]
  27. Ruchel R., Tegeler R., Trost M. 1982; A comparison of secretory proteinases from different strains of Candida albicans. Journal of Medical and Veterinary Mycology 20:233–244
    [Google Scholar]
  28. Ruchel R., Uhlemann K., Boning B. 1983; Secretion of acid proteinases by different species of the genus Candida. Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene (Abteilung I) 255:537–548
    [Google Scholar]
  29. Samaranayake L.P., Hughes A., Macfarlane T.W. 1984; The proteolytic potential of Candida albicans in human saliva supplemented with glucose. Journal of Medical Microbiology 17:13–22
    [Google Scholar]
  30. Schreiber B., Lyman C.A., Gurevich J., Needham C.A. 1985; Proteolytic activity of Candida albicans and other yeasts. Diagnostic Microbiology and Infectious Diseases 3:1–5
    [Google Scholar]
  31. Sessoms D.B., Lilly W.W. 1986; Derepressibleproteolytic activity in homokaryotic hyphae of Schizophyllum commune. Experimental Mycology 10:294–300
    [Google Scholar]
  32. Shepherd M.G., Sullivan P.A. 1976; The production and growth characteristics of yeast and mycelial forms of Candida albicans in continuous culture. Journal of General Microbiology 93:361–370
    [Google Scholar]
  33. Staib F. 1965; Serum-protein as nitrogen source for yeast-like fungi. Journal of Medical and Veterinary Mycology 4:187–193
    [Google Scholar]
  34. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America 76:4350–4354
    [Google Scholar]
  35. Wickerham L.J. 1946; A critical review of the nitrogen assimilation tests commonly used in the classification of yeasts. Journal of Bacteriology 52:293–301
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-136-4-687
Loading
/content/journal/micro/10.1099/00221287-136-4-687
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error