1887

Abstract

The ability to degrade aromatic amines and -toluate (Tdn phenotype), encoded by plasmid pTDN1, was lost from hosts after subculture in benzoate, succinate, acetate and glucose minimal medium, the fastest rate of loss occurring where benzoate was the substrate. Tdn cells had either lost the entire pTDN1 plasmid or suffered a recombinational deletion of a specific 26 kbp region. Proportional increase of Tdn cells resulted from their growth-rate advantage, and additionally, where benzoate was the substrate, from its metabolism via the chromosomal -cleavage pathway incorporating a short lag phase. The ratio of whole plasmid loss to deletion was substrate and pH dependent. Deletion of catabolic genes was not required for loss of pTDN1 but by comparison was a prerequisite for loss of TOL plasmid pWW0. It appeared that -toluate and benzoate were channelled via chromosomally encoded benzoate oxygenase and dihydroxycyclohexadiene carboxylate dehydrogenase prior to pTDN1 encoded -cleavage.

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1990-04-01
2021-10-27
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References

  1. Bayley S.A., Duggleby C.J., Worsey M.J., Williams P.A., Hardy K.G., Broda P. 1977; Two modes of loss of the TOL function from Pseudomonas putida mt-2. Molecular and General Genetics 154:203–204
    [Google Scholar]
  2. Clarke P.H., Laverack P.D. 1984; Growth characteristics of Pseudomonasstrains carrying catabolic plasmids and their cured derivatives. FEMS Microbiology Letters 24:109–112
    [Google Scholar]
  3. Cuskey S.M., Sprenkle A.B. 1988; Benzoate-dependent induction from the OP2 operator-promoter region of the TOL plasmid pWW0 in the absence of known plasmid regulatory genes. Journal of Bacteriology 170:3742–3746
    [Google Scholar]
  4. Eaton R.W., Ribbons D.W. 1982; Metabolism of dibutylphtha- late and phthalate by Micrococcus sp. Strain 12B. Journal of Bacteriology 151:48–57
    [Google Scholar]
  5. Franklin F.C.H., Bagdasarian M., Bagdasarian M.M., Timmis K.N. 1981; Molecular and functional analysis of the TOL plasmid pWWO from Pseudomonas putida and cloning of genes for the entire regulated aromatic ring meta cleavage pathway. Proceedings of the National Academy of Sciences of the United States of America 78:7458–7462
    [Google Scholar]
  6. Hermann M., Garg G.K., Gunsalus I.C. 1979; Fertility factors in Pseudomonas putida: selection and properties of high-frequency transfer and chromosomal donors. Journal of Bacteriology 137:1023–1033
    [Google Scholar]
  7. Keil H., Williams P.A. 1985; A new class of TOL plasmid deletion mutants in Pseudomonas putida MT15 and their reversion by tandem gene amplification. Journal of General Microbiology 131:1023–1033
    [Google Scholar]
  8. Keshavarz T., Lilly M.D., Clarke P.H. 1985; Stability of a catabolic plasmid in continuous culture. Journal of General Microbiology 131:1193–1203
    [Google Scholar]
  9. Kunz D.A., Chapman P.J. 1981; Isolation and characterization of spontaneously occurring TOL plasmid mutants of Pseudomonas putida HSI. Journal of Bacteriology 146:952–962
    [Google Scholar]
  10. Lakshmi V.V., Padma Sridhar, Polasa H. 1987; Elimination of multidrug-resistant plasmid in bacteria by plumbagin, a compound derived from a plant. Current Microbiology 16:159–161
    [Google Scholar]
  11. Lakshmi V.V., Padma Sridhar, Polasa H. 1989; Loss of plasmid linked antibiotic resistance in Escherichia coli on treatment with some phenolic compounds. FEMS Microbiology Letters 57:275–278
    [Google Scholar]
  12. Mcclure N.C., Venables W.A. 1986; Adaptation of Pseudomonas putida mt-2 to growth on aromatic amines. Journal of General Microbiology 132:2209–2218
    [Google Scholar]
  13. Mcclure N.C., Venables W.A. 1987; pTDNl, a catabolic plasmid involved in aromatic amine catabolism in Pseudomonas putida mt-2. Journal of General Microbiology 133:2073–2077
    [Google Scholar]
  14. Meulien P., Downing R.G., Broda P. 1981; Excision of the 40 kb segment of the TOL plasmid from Pseudomonas putida mt-2 involves direct repeats. Molecular and General Genetics 184:97–101
    [Google Scholar]
  15. Nakazawa T., Yokota T. 1973; Benzoate metabolism in Pseudomonas putida (arvilla) mt-2: demonstration of two benzoate pathways. Journal of Bacteriology 115:262–275
    [Google Scholar]
  16. Pickup R.W., Williams P.A. 1982; Spontaneous deletions in the TOL plasmid pWW20 which give rise to the B3 regulatory mutants of Pseudomonas putida MT20. Journal of General Microbiology 128:1385–1390
    [Google Scholar]
  17. Pickup R.W., Lewis R.J., Williams P.A. 1983; Pseudomonassp. MT14, a soil isolate which contains two large catabolic plasmids, one a TOL plasmid and one coding for phenylacetate catabolism and mercury resistance. Journal of General Microbiology 129:153–158
    [Google Scholar]
  18. Saint C.P., Mcclure N.C., Venables W.A. 1990; Physical map of the aromatic amine and m-toluate catabolic plasmid pTDN 1 in Pseudomonas putida: location of a unique meta-cleavage pathway. Journal of General Microbiology 136:615–625
    [Google Scholar]
  19. Stephens G.M., Dalton H. 1987; The effect of lipophilic weak acids on the segregational stability of TOL plasmids in Pseudomonas putida. Journal of General Microbiology 133:1891–1899
    [Google Scholar]
  20. Stephens G.M., Dalton H. 1988; The effect of lipophilic weak acids on the segregational stability of TOL plasmids in Pseudomonas putida MT15 during growth in chemostat culture. FEMS Microbiology Letters 55:175–180
    [Google Scholar]
  21. Wheatcroft R., Williams P.A. 1981; Rapid methods for the study of both stable and unstable plasmids in Pseudomonas. Journal of General Microbiology 124:433–437
    [Google Scholar]
  22. Williams P.A., Murray K. 1974; Metabolism of benzoate and the methylbenzoates by Pseudomonas putida (arvilla) mt-2: evidence for the existence of a TOL plasmid. Journal of Bacteriology 120:4l6–423
    [Google Scholar]
  23. Williams P.A., Worsey M.J. 1976; Ubiquity of plasmids in coding for toluene and xylene metabolism in soil bacteria: evidence for the existence of new TOL plasmids. Journal of Bacteriology 125:818–828
    [Google Scholar]
  24. Williams P.A., Taylor S.D., Gibb L.E. 1988; Loss of toluene- xylene catabolic genes of TOL plasmid pWWO during growth of Pseudomonas putida on benzoate is due to a selective growth advantage of ‘cured’ segregants. Journal of General Microbiology 134:2039–2048
    [Google Scholar]
  25. Worsey M.J., Williams P.A. 1977; Characterization of a spontaneously occurring mutant of the TOL20 plasmid in Pseudomonas putida MT20: possible regulatory implications. Journal of Bacteriology 130:1149–1158
    [Google Scholar]
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