1887

Abstract

A neamine-resistant mutant of LT2 with altered translational fidelity was isolated. The phenomenon was expressed in severe restriction of amber suppressor activity as well as in decreased misreading of poly(U) RNA The mutation conferring resistance to neamine was mapped at 72 min on the genetic map, where some of the ribosomal genes have already been mapped. This location indicates that the neamine-resistant phenotype as well as an altered translational fidelity could be a consequence of an alteration of the ribosomal structure.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-136-2-249
1990-02-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/136/2/mic-136-2-249.html?itemId=/content/journal/micro/10.1099/00221287-136-2-249&mimeType=html&fmt=ahah

References

  1. Bachmann B. J., Low K. B. 1980; Linkage map of Escherichia coli K-12. Microbiological Reviews 44:1–56
    [Google Scholar]
  2. Bollen A, Cabezon T., Dewilde M., Villaroel R., Herzog A. 1975; Alteration of ribosomal protein S17 by mutation linked to neamine resistance in E. coli . Journal of Molecular Biology 99:795–806
    [Google Scholar]
  3. Cabezon T., Herzog A., Dewilde M., Villaroel R., Bollen A. 1976; Cooperative control of translational fidelity by ribosomal proteins in E. coli. III. A ram mutation in the structural gene for protein S5 (rpsE). Molecular and General Genetics 144:59–62
    [Google Scholar]
  4. Dabbs E. R. 1978; Mutational alterations in 50 proteins of E. coli ribosome. Molecular and General Genetics 165:73–78
    [Google Scholar]
  5. Dewilde M., Cabezon T., Herzog A., Bollen A. 1975; Cooperative control of translational fidelity by ribosomal proteins in Escherichia coli. I. Properties of ribosomal mutants whose resistance to neamine is the cumulative effect of two distinct mutations. Molecular and General Genetics 142:19–33
    [Google Scholar]
  6. Gorini L. 1974; Streptomycin and misreading of the genetic code. In Ribosomes, pp 791–803 Nomura M., Tissières A., Lengyel P. Edited by Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  7. Kirsebom L. A., Isaksson L. A. 1985; Involvement of ribosomal protein L7/L12 in control of translational accuracy. Proceedings of the National Academy of Sciences of the United States of America 82:717–721
    [Google Scholar]
  8. Kirsebom L. A., Amons R., Isaksson L. A. 1986; Primary structures of mutationaUy altered ribosomal protein L7/L12 in control of translational accuracy. European Journal of Biochemistry 156:669–675
    [Google Scholar]
  9. Küchberger R., Piepbrsberg W., Petzet A., Buckel P., Böck A. 1979; Alteration of ribosomal protein L6 in gentamicin resistant strains of Escherichia coli. Effects on translational fidelity of protein synthesis. Biochemistry 18:187–193
    [Google Scholar]
  10. Matkovic B., Herzog A., Bollen A., Topisirovic L. 1980; Translational fidelity in Escherichia coli: antagonistic effects of neaA and ramC gene products on the ribosome function. Molecular and General Genetics 179:135–139
    [Google Scholar]
  11. Miller J. H. 1972 Experiments in Molecular Genetics. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  12. Pestka S., Marchal R., Nirenberg M. W. 1975; RNA code words and protein synthesis. V. Effect of the streptomycin on the formation of ribosome sRNA complexes. Proceedings of the National Academy of Sciences of the United States of America 53:639–646
    [Google Scholar]
  13. Piepersberg W., Böck A., Wittmann H. G. 1975; Effect of different mutations in the ribosomal protein S5 of E. coli on translational fidelity. Molecular and General Genetics 140:91–100
    [Google Scholar]
  14. Ravel J., Shorey R.A.L. 1971; GTP-dependent binding of aminoacyl-tRNA to Escherichia coli ribosomes. Methods in Enzymology 20:306–316
    [Google Scholar]
  15. Rosset R., Gorini L. 1969; A ribosomal ambiguity mutation. Journal of Molecular Biology 39:95–101
    [Google Scholar]
  16. Sanderson K. E., Roth J. 1983; Linkage map of Salmonella typhimurium . Microbiological Reviews 47:410–453
    [Google Scholar]
  17. Tapio S., Isaksson L. A. 1989; Antagonistic effects of mutant elongation factor Tu and ribosomal protein SI2 on control of translational accuracy, suppression and cellular growth. Biochemie 70:273–281
    [Google Scholar]
  18. Topisirovic L., Villaroel R., Dewilde M., Herzog A., Cabezon T., Bollen A. 1977; Translational fidelity in E. coli: contrasting role of neaA and ramA gene products in the ribosome functioning. Molecular and General Genetics 151:89–94
    [Google Scholar]
  19. Vogel H. J., Bonner D. M. 1956; Acetylomithinase of Escherichia coli: partial purification and some properties. Journal of Biological Chemistry 218:97–106
    [Google Scholar]
  20. Winston F., Botstein D., Miller J. H. 1979; Characterization of amber and ochre suppressors in Salmonella typhimurium . Journal of Bacteriology 137:433–439
    [Google Scholar]
  21. Wood W. B., Berg P. 1962; The effect of enzymatically synthesized ribonucleic acid on amino acid incorporation by soluble protein-ribosome system from Escherichia coli . Proceedings of the National Academy of Sciences of the United States of America 48:94–104
    [Google Scholar]
  22. Yamada T., Davis J. 1971; A genetic and biochemical study of streptomycin- and spectinomycin-resistance in Salmonella typhimurium . Molecular and General Genetics 110:197–210
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-136-2-249
Loading
/content/journal/micro/10.1099/00221287-136-2-249
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error