1887

Abstract

Studies on the enzymes of the cellulase complex of the thermophilic fungus var. are described. A genomic library was constructed in the phage vector EMBL 4, and from this library two clones were isolated using as a probe the cloned (exoglucanase, EC 3.2.1.91) gene of , a cellulolytic basidiomycete fungus. These clones were analysed by restriction mapping and Southern blotting, and one of them (3) was sub-cloned into the M13 phage vectors mp18 and mp19. The gene sequence was determined by the dideoxy chain-termination method. Sequence comparison with the equivalent genes from and was made: in terms of primary sequence there is about 60% homology between the three species. Secondary structure prediction of the sequence was also computed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-136-12-2569
1990-12-01
2021-05-13
Loading full text...

Full text loading...

/deliver/fulltext/micro/136/12/mic-136-12-2569.html?itemId=/content/journal/micro/10.1099/00221287-136-12-2569&mimeType=html&fmt=ahah

References

  1. Akrigg D., Bleasby A. J., Dix N. I. M., Findlay J. B. C., North A. C. T., Parry-Smith D., Wootton J. C., Blundell T. L., Gadner S. P., Hayes F., Islam S., Sternberg M. J. E., Thornton J. M., Tickle I. J. 1988; A protein sequence/structure database. Nature; London: 335745–746
    [Google Scholar]
  2. Araujo E. F., Barros E. G., Caldas R. A., Silva D. O. 1983; Beta-glucosidase activity of a thermophilic cellulolytic fungus, Humicola sp. Biotechnology Letters 5:781–784
    [Google Scholar]
  3. Azevedo J. L., Costa S. O. P. 1973 Exercícios Práticos de Genética Saõ Paulo: Edicions Universidad de Saõ Paulo;
    [Google Scholar]
  4. Azevedo M. DE O., Radford A. 1990; Sequence of the cbh-1 gene of Humicola grisea var. thermoidea . Nucleic Acids Research 18:668
    [Google Scholar]
  5. Chou P. Y., Fasman D. G. 1978; Prediction of the secondary structure of proteins from their amino acid sequence. Advances in Enzymology 47:45–148
    [Google Scholar]
  6. Chung C. T., Niemala S. L., Miller R. H. 1989; One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution. Proceedings of the National Academy of Sciences of the United States of America 86:2172–2175
    [Google Scholar]
  7. Eberhardt B. M. 1961; Exogenous enzymes of Neurospora conidia and mycelia. Journal of Cellular and Comparative Physiology 58:11–16
    [Google Scholar]
  8. Eberhardt B. M., Beck R. S. 1973; Induction of β-glucosidases in Neurospora crassa . Journal of Bacteriology 116:295–303
    [Google Scholar]
  9. Frischauf A. M., Lehrach H., Poustka A., Murray N. 1983; Lambda replacement vectors carrying polylinker sequences. Journal of Molecular Biology 170:827–842
    [Google Scholar]
  10. Garnier J., Osguthorpe D. J., Robson B. 1976; Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins. Journal of Molecular Biology 120:97–120
    [Google Scholar]
  11. Gong C. S., Tsao G. T. 1980; Cellulase biosynthesis and regulation. Annual Reports on Fermentation Processes 3:111–140
    [Google Scholar]
  12. Knowles J., Lehtovaara P., Teeri T. 1987; Cellulase families and their genes. Trends in Biotechnology 5:255–261
    [Google Scholar]
  13. Kyte J., Doolittle R. F. 1982; A simple method for displaying the hydropathic character of a protein. Journal of Molecular Biology 157:105–132
    [Google Scholar]
  14. Mandel M., Higa A. 1970; Calcium-dependent bacteriophage infection. Journal of Molecular Biology 53:159–162
    [Google Scholar]
  15. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning - a Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  16. Messing J. 1983; New M13 vectors for cloning. Methods in Enzymology 101c:20–79
    [Google Scholar]
  17. Perbal B. 1989 A Practical Guide to Molecular Cloning, 2nd edn. New York: Wiley;
    [Google Scholar]
  18. Pontecorvo G., Roper J. A., Forbes E. 1953; Genetic recombination without reproduction in Aspergillus niger . Journal of General Microbiology 8:198–210
    [Google Scholar]
  19. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences of the United States of America 74:5463–5467
    [Google Scholar]
  20. Shoemaker S., Schweickart V., Ladner M., Gelfand D., Kwok S., Myambo K., Innis M. 1983; Molecular cloning of exo-cellobiohydrolase 1 derived from Trichoderma reesei strain L27. Biol technology 1:691–696
    [Google Scholar]
  21. Sims P., James C., Broda P. 1988; The identification, molecular cloning and characterization of a gene from Phanerochaete chrysosporium that shows strong homology to the exo-cellobiohydrolase I gene of Trichoderma reesei . Gene 74:411–422
    [Google Scholar]
  22. Staden R. 1982; An interactive graphics program for comparing and aligning nucleic acid and amino acid sequences. Nucleic Acids Research 10:2951–2961
    [Google Scholar]
  23. Svensson B., Larsen K., Gunnarsson A. 1986; Characterisation of a glucoamylase G2 from Aspergillus niger . European Journal of Biochemistry 154:497–502
    [Google Scholar]
  24. Teeri T., Salovuori I., Knowles J. 1983; The molecular cloning of the major cellulase gene from Trichoderma reesei . Bio/technology 1:696–699
    [Google Scholar]
  25. Yamashita I., Suzuki K., Fukui S. 1985; Nucleotide sequence of the extracellular glucoamylase gene STA1 in the yeast Saccharomyces cerevisiae . Journal of Bacteriology 161:567–573
    [Google Scholar]
  26. Yanisch-Perron C., Vieira J., Messing J. 1985; Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103–119
    [Google Scholar]
  27. Yazdi M. T., Woodward J. R., Radford A. 1990a; Cellulase production by Neurospora crassa: induction and optimisation of the complex. Enzyme and Microbial Technology 12:116–119
    [Google Scholar]
  28. Yazdi M. T., Radford A., Keen J. N., Woodward J. R. 1990b; Cellulase production by Neurospora crassa: purification and characterisation of cellulolytic enzymes. Enzyme and Microbial Technology 12:120–123
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-136-12-2569
Loading
/content/journal/micro/10.1099/00221287-136-12-2569
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error