1887

Abstract

Summary: Studies on the enzymes of the cellulase complex of the thermophilic fungus var. are described. A genomic library was constructed in the phage vector EMBL 4, and from this library two clones were isolated using as a probe the cloned (exoglucanase, EC 3.2.1.91) gene of , a cellulolytic basidiomycete fungus. These clones were analysed by restriction mapping and Southern blotting, and one of them (λ3) was sub-cloned into the M13 phage vectors mp18 and mp19. The gene sequence was determined by the dideoxy chain-termination method. Sequence comparison with the equivalent genes from and was made: in terms of primary sequence there is about 60% homology between the three species. Secondary structure prediction of the sequence was also computed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-136-12-2569
1990-12-01
2019-10-18
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-136-12-2569
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error