Summary: Studies on the enzymes of the cellulase complex of the thermophilic fungus var. are described. A genomic library was constructed in the phage vector EMBL 4, and from this library two clones were isolated using as a probe the cloned (exoglucanase, EC gene of , a cellulolytic basidiomycete fungus. These clones were analysed by restriction mapping and Southern blotting, and one of them (λ3) was sub-cloned into the M13 phage vectors mp18 and mp19. The gene sequence was determined by the dideoxy chain-termination method. Sequence comparison with the equivalent genes from and was made: in terms of primary sequence there is about 60% homology between the three species. Secondary structure prediction of the sequence was also computed.


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