1887

Abstract

cell walls contain two major glycoprotein species, I and II, with molecular masses of 2 × 10 and 5 × 10 Da respectively, as determined by gel filtration chromatography and PAGE. The ratio of sugar to protein is higher in species I than in species II. Much of the sugar in both glycoproteins (about 85% in wild-type cells) is -linked to the peptide moiety. The morphological mutant is resistant to papulacandin B, and its cell wall contains less glycoprotein II (but not less glycoprotein I) than the parental wild-type strain, although glycoprotein II is still synthesized and released into the growth medium. Papulacandin B largely reverses the morphological alteration of the mutant, and returns the ratio between species I and II to about that found in the parental strain, although the absolute amount of species II is still lower in the mutant. The results point to the importance of the relative amounts of the different wall polymers in determining cell morphology.

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1990-11-01
2024-04-26
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References

  1. Baguley B. C., Rommele G., Grunner J., Wehrli W. 1979; Papulacandin B: an inhibitor of glucan synthesis in yeast sphero-plasts. European Journal of Biochemistry 97:345–351
    [Google Scholar]
  2. Bush D. A., Horisberger M., Horman I., Wursch P. 1974; The wall structure of Schizosaccharomyces pombe . Journal of General Microbiology 81:199–206
    [Google Scholar]
  3. Duran A., Varona R., Perez P., Garcia-Acha I. 1984; Biosynthesis of fungal wall β-glucan and its inhibition by antifungal agents. In Microbial Cell Wall Synthesis and Autolysis pp. 297–305 Nombela C. Edited by Amsterdam: Elsevier;
    [Google Scholar]
  4. Elorza M. V., Murgui A., Sentandreu R. 1985; Dimorphism in Candida albicans: contribution of mannoproteins to the architecture of yeast and mycelial cell walls. Journal of General Microbiology 131:2209–2216
    [Google Scholar]
  5. Elorza M. V., Marcilla A., Sentandreu R. 1988; Wall mannoproteins of the yeast and mycelial cells of Candida albicans: nature of the glycosidic bonds and polydispersity of their mannan moieties. Journal of General Microbiology 134:2393–2403
    [Google Scholar]
  6. Frevert J., Ballou C. E. 1985; Saccharomyces cerevisiae structural cell wall mannoprotein. Biochemistry 24:753–759
    [Google Scholar]
  7. Herrero E., Valentin E., Sentandreu R. 1985; Effect of α-factor on individual wall mannoproteins from Saccharomyces cerevisiae a cells. FEMS Microbiology Letters 27:293–297
    [Google Scholar]
  8. Herrero E., Sanz P., Sentandreu R. 1987; Cell wall proteins liberated by Zymolyase from several ascomycetous and imperfect yeasts. Journal of General Microbiology 133:2895–2903
    [Google Scholar]
  9. Horisberger M., Rosset J. 1977; Localization of α-galactoman-nan on the surface of Schizosaccharomyces pombe cells by scanning electron microscopy. Archives of Microbiology 112:123–126
    [Google Scholar]
  10. Kitamura K., Kaneko T., Yamamoto Y. 1974; Lysis of viable yeast cells by enzymes of Arthrobacter luteus. II. Purification and properties of an enzyme, Zymolyase, which lyses viable yeast cells. Journal of General and Applied Microbiology 20:323–344
    [Google Scholar]
  11. Klebl F., Tanner W. 1989; Molecular cloning of a cell wall exo-β-1,3-glucanase from Saccharomyces cerevisiae . Journal of Bacteriology 171:6259–6264
    [Google Scholar]
  12. Kukuruzinska M. A., Bergh M. L. E., Jackson B. J. 1987; Protein glycosylation in yeast. Annual Review of Biochemistry 56:915–944
    [Google Scholar]
  13. Maley F., Trimble R. B., Tarentino A. L., Plummer T. H. Jr 1989; Characterization of glycoproteins and their associated oligosaccharides through the use of endoglycosidases. Analytical Biochemistry 180:195–204
    [Google Scholar]
  14. Manners D. J., Meyer M. T. 1977; The molecular structures of some glucans from the cell walls of Schizosaccharomyces pombe . Carbohydrates Research 57:189–203
    [Google Scholar]
  15. Manners D. J., Masson A. J., Patterson J. C. 1974; The heterogeneity of glucan preparations from the walls of various agents. Journal of General Microbiology 80:411–417
    [Google Scholar]
  16. Murgui A., Elorza M. V., Sentandreu R. 1985; Effect of papulacandin B and calcofluor white on the incorporation of mannoproteins in the wall of Candida albicans blastospores. Biochimica et Biophysica Acta 841:215–222
    [Google Scholar]
  17. Pastor F. I. J., Valentin E., Herrero E., Sentandreu R. 1984; Structure of Saccharomyces cerevisiae cell wall mannoproteins released by Zymolyase and their contribution to wall architecture. Biochimica et Biophysica Acta 802:292–300
    [Google Scholar]
  18. Peberdy J. F. 1985; Mycolytic enzymes. In Fungal Protoplasts pp. 31–44 Peberdy J. F., Ferenczy L. Edited by New York: Marcel Dekker;
    [Google Scholar]
  19. Russell P., Nurse P. 1986; Schizosaccharomyces pombe and Saccharomyces cerevisiae: a look at yeasts divided. Cell 45:781–782
    [Google Scholar]
  20. Valentín E., Herrero E., Rico H., Miragall F., Sentandreu R. 1987; Cell wall mannoproteins during the population growth phases in Saccharomyces cerevisiae . Archives of Microbiology 148:88–94
    [Google Scholar]
  21. Varona R., Perez P., Duran A. 1983; Effect of papulacandin B on β-glucan synthesis in Schizosaccharomyces pombe . FEMS Microbiology Letters 20:243–247
    [Google Scholar]
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