SUMMARY: The development of a protoplast manipulation protocol for the industrially important bacterium , which produces the β-lactamase inhibitor clavulanic acid, made possible a preliminary genetic mapping study based on protoplast fusion crosses. A preliminary position for 11 markers on the genetic map is proposed. Fusion progeny were characterized by random spore analysis because the markers present in the strains were not amenable to the conventional four-on-four selection procedure. Whilst the resulting map is similar to that derived by conjugation for and , further analysis of the markers is required to confirm these observations.


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