1887

Abstract

The development of a protoplast manipulation protocol for the industrially important bacterium , which produces the -lactamase inhibitor clavulanic acid, made possible a preliminary genetic mapping study based on protoplast fusion crosses. A preliminary position for 11 markers on the genetic map is proposed. Fusion progeny were characterized by random spore analysis because the markers present in the strains were not amenable to the conventional four-on-four selection procedure. Whilst the resulting map is similar to that derived by conjugation for and , further analysis of the markers is required to confirm these observations.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-135-8-2299
1989-08-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/135/8/mic-135-8-2299.html?itemId=/content/journal/micro/10.1099/00221287-135-8-2299&mimeType=html&fmt=ahah

References

  1. Aharonowitz Y., Demain A.L. 1977; Genetic recombination in the cephamycin producer, Streptomyces clavuligerus.. European Journal of Applied Microbiology 4:125–130
    [Google Scholar]
  2. Arst H.N. 1968; Genetic analysis of the first steps of sulphate metabolism in Aspergillus nidulans.. Nature, London 219:268–270
    [Google Scholar]
  3. Bailey C.R., Butler M.J., Normansell I.D., Rowlands R.T., Winstanley D.J. 1984; Cloning a Streptomyces clavuligerus genetic locus involved in clavulanic acid biosynthesis.. Biotechnology 2:808–811
    [Google Scholar]
  4. Baltz R.H. 1978; Genetic recombination in Streptomyces fradiae by protoplast fusion and cell regeneration.. Journal of General Microbiology 107:93–102
    [Google Scholar]
  5. Baltz R.H. 1980; Genetic recombination by protoplast fusion in Streptomyces.. Developments in Industrial Microbiology 21:43–54
    [Google Scholar]
  6. Friend E.J., Hopwood D.A. 1971; The linkage map of Streptomyces rimosus.. Journal of General Microbiology 68:187–197
    [Google Scholar]
  7. Hopwood D.A. 1972; Genetic analysis in microorganisms.. Methods in Microbiology 7B:27–38
    [Google Scholar]
  8. Hopwood D.A., Chater K.F. 1984; Streptomyces.. In Genetics and Breeding of Industrial Microorganisms, pp 7–42 Ball C. Edited by Boca Raton: CRC Press;
    [Google Scholar]
  9. Hopwood D.A., Wright H.M. 1978; Bacterial protoplast fusion: recombination in fused protoplasts of Streptomyces coelicolor.. Molecular and General Genetics 162:307–317
    [Google Scholar]
  10. Hopwood D.A., Wright H.M., Bibb M.J., Cohen S.N. 1977; Genetic recombination through protoplast fusion in Streptomyces.. Nature, London 268:171–174
    [Google Scholar]
  11. Hopwood D.A., Kieser T., Wright H.M., Bibb M.J. 1983; Plasmids, recombination and chromosome mapping in Streptomyces lividans 66.. Journal of General Microbiology 129:2257–2269
    [Google Scholar]
  12. Hopwood D.A., Bibb M.J., Chater K.F., Kieser T., Bruton C.J., Kieser H.M., Lydiate D.J., Smith C.P., Ward J.M., Schrempf H. 1985 Genetic Manipulation of Streptomyces. A Laboratory Manual. Norwich: The John Innes Foundation;
    [Google Scholar]
  13. Hranueli D., Pigac J., Smokvina T., Alacevic M. 1983; Genetic interactions in Streptomyces rimosus mediated by conjugation and by protoplast fusion.. Journal of General Microbiology 129:1415–1422
    [Google Scholar]
  14. Illing G.T. 1987 Protoplast fusion and regeneration in Streptomyces clavuligerus. PhD thesis, University of Nottingham, Nottingham
    [Google Scholar]
  15. Illing G.T., Onions J.P. 1986; Genetic analysis of a sexual or parasexual cross: Genetics, p.. Binary 7:33–36
    [Google Scholar]
  16. Illing G.T., Normansell I.D., Peberdy J.F. 1989; Protoplast isolation and regeneration in Streptomyces clavuligerus.. Journal of General Microbiology 135:2289–2297
    [Google Scholar]
  17. Keller U., Poschmann S., Krengel U., Klein-KAUF H., Kraepelin G. 1983; Studies of protoplast fusion in Streptomyces chrysomallus.. Journal of General Microbiology 129:1725–1731
    [Google Scholar]
  18. Keller U., Krengel U., Haese A. 1985; Genetic analysis in Streptomyces chrysomallus.. Journal of General Microbiology 131:1181–1191
    [Google Scholar]
  19. Kirby R. 1978; Genetic mapping of Streptomyces clavuligerus.. FEMS Microbiology tetters 3:177–180
    [Google Scholar]
  20. Pigac J., Hranueli D., Smokvina T., Alacevic M. 1982; Optimal cultural and physiological conditions for handling Streptomyces rimosus protoplasts.. Applied and Environmental Microbiology 44:1178–1186
    [Google Scholar]
  21. Queener S.W., Baltz R.H. 1979; Genetics of industrial microorganisms.. Annual Report on Fermentation Processes 3:5–45
    [Google Scholar]
  22. Rhodes M. 1986; Genetic recombination and maps for Streptomyces.. In The Bacteria, pp 27–60 Queener S. W., Day L. E. Edited by London: Academic Press;
    [Google Scholar]
  23. Saunders G., Allsop A.E., Holt G. 1982; Modem developments in mutagenesis.. Journal of Chemical Technology and Biotechnology 32:254–264
    [Google Scholar]
  24. Sermonti G., Spada-Sermonti I. 1955; Genetic recombination in Streptomyces.. Nature, London 176:121
    [Google Scholar]
  25. Wesseling A.C., Lago B.D. 1981; Strain improvement by genetic recombination of cepha- mycin producers, Nocardialactamdurans and Streptomyces griseus.. Developments in Industrial Microbiology 22:31–40
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-135-8-2299
Loading
/content/journal/micro/10.1099/00221287-135-8-2299
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error