1887

Abstract

The transformation of uninucleate amoebae of into multinucleate plasmodia is under the control of a multiallelic mating-type locus, . Plasmodium formation usually occurs only when amoebal fusion brings together two different alleles within a single cell; such plasmodium formation is termed crossing. Mutations () in the locus permit haploid amoebae to self, that is to form plasmodia in clonal cultures without amoebal fusion. By constructing diploid amoebae heterozygous for alleles, it was shown that the mutant alleles and were each dominant to . Non-selfing revertants of mutants may be generated as a result of further mutations () within the locus. Sixty-one revertants of this type were studied, derived from three mutants. Some revertants were able to cross with a tester strain. These ‘class I’ revertants failed to cross with one another or with similar revertants of two mutants; they appear all to lack a single gene function ( ) that is necessary in plasmodium development. Other revertants failed to cross with the tester. These ‘class II’ revertants also failed to cross with one another, but showed several different patterns of crossing when mixed with other strains. The behaviour of some of the class II revertants suggests that they lack a further gene function ( ) necessary in plasmodium development. The mutations may affect a -acting regulator of .

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1989-05-01
2022-01-17
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References

  1. Adler r.N., Holt C.E. 1974a; Change in properties of Physarum polycephalum amoebae during extended culture. Journal of Bacteriology 120:532–533
    [Google Scholar]
  2. Adler P.N., Holt C.E. 1974b; Genetic analysis in the Colonia strain of Physarum polycephalum : heterothallic strains that mate with and are partially isogenic to the Colonia strain. Genetics 78:1051–1062
    [Google Scholar]
  3. Adler P.N., Holt C.E. 1975; Mating type and the differentiated state in Physarum polycephalum . Developmental Biology 43:240–253
    [Google Scholar]
  4. Adler P.N., Holt C.E. 1977; Mutations increasing asexual plasmodium formation in Physarum polycephalum . Genetics 87:401–420
    [Google Scholar]
  5. Adler P.N., Davidow L.S., Holt C.E. 1975; Life cycle variants of Physarum polycephalum that lack the amoeba stage. Science 190:65–67
    [Google Scholar]
  6. Anderson R.W. 1976 Analysis of the amoebal-plasmodial transition in Physarum polycephalum. PhD thesis University of Leicester; UK:
    [Google Scholar]
  7. Anderson R.W. 1977; A plasmodial colour mutation in the Myxomycete Physarum polycephalum . Genetical Research 30301–306
    [Google Scholar]
  8. Anderson R.W. 1979; Complementation of amoebal-plasmodial transition mutants in Physarum polycephalum . Genetics 91:409–419
    [Google Scholar]
  9. Anderson R.W., Dee J. 1977; Isolation and analysis of amoebal-plasmodial transition mutants in the Myxomycete Physarum polycephalum . Genetical Research 29:21–34
    [Google Scholar]
  10. Anderson R.W., Holt C.E. 1981; Revertants of selfing (gad) mutants in Physarum polycephalum . Developmental Genetics 2:253–267
    [Google Scholar]
  11. Anderson R.W., Hutchins G. 1986; Uncertainty in the mapping of gad mutations. Physarum Newsletter 18:6–8
    [Google Scholar]
  12. Anderson R.W., Youngman P.J. 1985; Complementation of npf mutations in diploid amoebae of Physarum polycephalum: the basis for a general method of complementation analysis at the amoebal stage. Genetical Research 45:21–35
    [Google Scholar]
  13. Anderson R.W., Gray A., Hutchins G., Price J. 1983; Preservation of Physarum polycephalum amoebae with anhydrous silica gel. Physarum Newsletter 15:3
    [Google Scholar]
  14. Anderson R.W., Dee J., Gull K. 1986a; Cellular transformations of myxamoebae. In The Molecular Biology of Physarum polycephalum pp. 111–130 Dove W.F., Dee J., Hatano S., Haugli F.B., Wohlfarth-Bottemann K.-E. Edited by New York: Plenum Press;
    [Google Scholar]
  15. Anderson R.W., Burland T.G., Dee J. 1986b; Genetic nomenclature for Physarum polycephalum . Physarum Newsletter 18:2–5
    [Google Scholar]
  16. Collins O.R. 1975; Mating types in five isolates of Physarum polycephalum . Mycologia 67:98–107
    [Google Scholar]
  17. Collins O.R., Tang H.-C. 1977; New mating types in Physarum polycephalum . Mycologia 69:421–423
    [Google Scholar]
  18. Cooke D.J., Dee J. 1975; Methods for the isolation and analysis of plasmodial mutants in Physarum polycephalum . Genetical Research 24:175–187
    [Google Scholar]
  19. Davidow L.S., Holt C.E. 1977; Mutants with decreased differentiation to plasmodia in Physarum polycephalum . Molecular and General Genetics 155:291–300
    [Google Scholar]
  20. Dee J. 1966; Multiple alleles and other factors affecting plasmodium formation in the true slime mould Physarum polycephalum Schw. Journal of Protozoology 13:610–616
    [Google Scholar]
  21. Dee J. 1978; A gene unlinked to mating-type affecting crossing between strains of Physarum polycephalum . Genetical Research 31:85–92
    [Google Scholar]
  22. Dee J. 1987; Genes and development in Physarum . Trends in Genetics 3:208–213
    [Google Scholar]
  23. Dee J., Anderson R.W. 1984; The effect of ploidy on the stability of plasmodial heterokaryons in Physarum polycephalum . Journal of General Microbiology 131:1167–1179
    [Google Scholar]
  24. Gorman J.A., Dove W.F., Shaibe E. 1979; Mutations affecting the initiation of plasmodial development in Physarum polycephalum . Developmental Genetics 1:47–60
    [Google Scholar]
  25. Haugli F.B., Dove W.F. 1972; Mutagenesis and mutant selection in Physarum polycephalum . Molecular and General Genetics 118:109–124
    [Google Scholar]
  26. Honey N.K., Poulter R.T.M., Teale D.M. 1979; Genetic regulation of differentiation in Physarum polycephalum . Genetical Research 34:131–142
    [Google Scholar]
  27. Honey N.K., Poulter R.T.M., Winter P.J. 1981; Selfing mutants from heterothallic strains of Physarum polycephalum . Genetical Research 37:113–121
    [Google Scholar]
  28. Honey N.K., Poulter R.T.M., Aston R.J. 1982; Non-selfing mutants from selfing (Het-)strains of Physarum polycephalum . Genetical Research 39:261–273
    [Google Scholar]
  29. Kawano S., Kuroiwa T., Anderson R.W. 1987; A third multiallelic mating-type locus in Physarum Polycephalum . Journal of General Microbiology 133:2539–2546
    [Google Scholar]
  30. Kirouac-Brunet J., Masson S., Pallotta D.J. 1981; Multiple allelism at the matB locus in Physarum polycephalum . Canadian Journal of Genetics and Cytology 239–16
    [Google Scholar]
  31. Mccurrach K., Glover L.A., Hardman N. 1988; Transient expression of a chloramphenicol acetyltransferase gene following transfection of Physarum polycephalum myxamoebae. Current Genetics 13:71–74
    [Google Scholar]
  32. Poulter R.T.M., Dee J. 1968; Segregation of factors controlling fusion between plasmodia of the true slime mould Physarum polycephalum . Genetical Research 12:71–79
    [Google Scholar]
  33. Shinnick T.M., Holt C.E. 1977; A mutation (gad) linked to mt and affecting plasmodium formation in Physarum polycephalum . Journal of Bacteriology 131:247–250
    [Google Scholar]
  34. Shinnick T.M., Pallotta D.J., Jones-Brown Y.R., Youngman P.J., Holt C.E. 1978; A gene, imz, affecting the pH sensitivity of zygote formation in Physarum polycephalum . Current Microbiology 1:163–166
    [Google Scholar]
  35. Shinnick T.M., Anderson R.W., Holt C.E. 1983; . Map and function of gad mutations in Physarum polycephalum . Genetical Research 42:41–57
    [Google Scholar]
  36. Truitt C.L., Hoffman C.S., Holt C.E. 1982; A gene, ale A, affecting the life cycle form expressed in Physarum polycephalum . Genetics 101:35–55
    [Google Scholar]
  37. Wheals A.E. 1970; A homothallic strain of the Myxomycete Physarum polycephalum . Genetics 66:623–633
    [Google Scholar]
  38. Youngman P.J. 1979; Studies concerning the regulation of plasmodium formation in Physarum polycephalum and other Myxomycetes. PhD thesis Massachusetts Institute of Technology; USA:
    [Google Scholar]
  39. Youngman P.J., Pallotta D.J., Hosler B., Struhl G., Holt C.E. 1979; A new mating compatibility locus in Physarum polycephalum . Genetics 91:683–693
    [Google Scholar]
  40. Youngman P.J., Anderson R.W., Holt C.E. 1981; Two multiallelic mating compatibility loci separately regulate zygote formation and zygote differentiation in the Myxomycete Physarum polycephalum . Genetics 91:513–530
    [Google Scholar]
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