1887

Abstract

SDS-PAGE of the outer-membrane (OM) proteins of P205 grown under iron-sufficient conditions revealed three major proteins of 40, 37 and 13 kDa. In addition, growth under conditions of iron-restriction resulted in the expression of at least four iron-repressible OM proteins (IROMPs) of 72, 81, 88 and 90 kDa. OM proteins of 40 and 13 kDa were non-covalently associated with peptidoglycan and were resistant to digestion with trypsin. A 38 kDa peptidoglycan-associated protein, which was masked by the abundant 37 kDa protein, was also observed following tryptic digestion of whole cells or OMs. Neither the 37 kDa protein (which was heat-modifiable) nor the IROMPs were peptidoglycan-associated, and both were cleaved following treatment of whole cells with trypsin, indicating that they are exposed at the cell surface. A variety of IROMPs from five other strains was also observed. In each strain, both the IROMPs and a major protein of 37 kDa were exposed at the cell surface.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-135-2-445
1989-02-01
2024-12-07
Loading full text...

Full text loading...

/deliver/fulltext/micro/135/2/mic-135-2-445.html?itemId=/content/journal/micro/10.1099/00221287-135-2-445&mimeType=html&fmt=ahah

References

  1. Albritton W.L. 1982; Infections due toHaemophilus species other than H. influenzae . Annual Review of Microbiology 11:199–216
    [Google Scholar]
  2. Van Alphen L., Riemens T., Poolman J., Zanen H.C. 1983; Characteristics of major outer membrane proteins ofHaemophilus influenzae . Journal of Bacteriology 155:878–885
    [Google Scholar]
  3. Behr M.G., Schnaitman C.A., Pugsley A. 1980; Major heat-modifiable outer membrane protein in gram-negative bacteria: comparison with the OmpA protein ofEscherichia coli . Journal of Bacteriology 143:906–913
    [Google Scholar]
  4. Black C. T., Kupperschmid J. P., West K. W., Grosfeld J. L. 1988; Haemophilus parainfluenzae infections in children, with a report of a unique case. Reviews of Infectious Diseases 10:342–346
    [Google Scholar]
  5. Bruun B., Christensen J.J., Kilian M. 1984; Bacteremia caused by a beta-lactamase producingHaemophilus parainfluenzae strain of a new biotype. Acta pathologica, microbiologica et immunologica scandinavica B92:135–138
    [Google Scholar]
  6. Burbach S., Kilian M., Mannheim W. 1986; Intra and intergeneric DNA relationships of the humanHaemophilus species. XIV International Congress of Microbiology, Manchester, UK, 7-13 th September 1986 45: (abstract).
    [Google Scholar]
  7. Darveau R.P., MacIntyre S., Buckley J.T., Hancock R.E.W. 1983; Purification and reconstitution in lipid bilayer membranes of an outer membrane pore forming protein ofAeromonas salmonicida . Journal of Bacteriology 156:1006–1011
    [Google Scholar]
  8. Griffiths E. 1987; The iron-uptake systems of pathogenic bacteria. In Iron and Infection; Molecular, Physiological and Clinical Aspects pp 69–137 Bullen J. J., Griffiths E. Edited by Chichester: Wiley;
    [Google Scholar]
  9. Griffiths E., Bullen J.J. 1987; Iron-binding proteins and host defence. In Iron and Infection; Molecular, Physiological and Clinical Aspects pp 171–210 Bullen J. J., Griffiths E. Edited by Chichester: Wiley;
    [Google Scholar]
  10. Hancock R.E.W. 1987; Role of porins in outer membrane permeability. Journal of Bacteriology 169:929–933
    [Google Scholar]
  11. Herrington D.A., Sparling P. F. 1985; Haemophilus influenzae can cause human transferrin as a sole source for required iron. Infection and Immunity 48:248–251
    [Google Scholar]
  12. Kilian M., Frederiksen W. 1980; Ecology ofHaemophilus, Pasteurella, and Actinobacillus . In Haemophilus, Pasteurella, and Actinobacillus pp Kilian M., Frederiksen W., Biberstein E. L. Edited by New York: Academic Press;
    [Google Scholar]
  13. Lugtenberg B., Van Alphen L. 1983; Molecular architecture and functioning of the outer membrane ofEscherichia coli and other Gram-negative bacteria. Biochimica et biophysica acta 737:51–115
    [Google Scholar]
  14. Neilands J.B. 1982; Microbial envelope proteins related to iron. Annual Review of Microbiology 36:285–309
    [Google Scholar]
  15. Osborn M.J., Wu H.C.P. 1980; Proteins of the outer membrane of Gram-negative bacteria. Annual Review of Microbiology 34:369–422
    [Google Scholar]
  16. Pidcock K.A., Wooten J.A., Daley B.A., Stull T.L. 1988; Iron acquisition by Haemophilus influenzae . Infection and Immunity 56:721–725
    [Google Scholar]
  17. Pohl S. 1980; DNA relatedness among members of Haemophilus, Pasteurella, and Actinobacillus . In Haemophilus, Pasteurella, and Actinobacillus pp 245–253 Kilian M., Biberstein W., Frederiksen E.L. Edited by New York: Academic Press;
    [Google Scholar]
  18. Roberts M.C., Mintz C.S., Morse S.A. 1986; Characterization ofHaemophilus parainfluenzaestrains with low-Mr or ladder-like lipopolysacchar-ides. Journal of General Microbiology 132:611–616
    [Google Scholar]
  19. Rycroft A.N., Taylor D.J. 1987; Preparation and characterization of envelope proteins fromHaemophilus pleuropneumoniae . Veterinary Microbiology 15:303–314
    [Google Scholar]
  20. Vachon V., Kristjanson D.N., Coulton J.W. 1988; Outer membrane porin protein ofHaemophilus influenzae type b: pore size and sub-unit structure. Canadian Journal of Microbiology 34:134–140
    [Google Scholar]
  21. Williams P., Brown M.R.W. 1986; Influence of iron-restriction on growth and the expression of outer membrane proteins byHaemophilus influenzaeand H. parainfluenzae . FEMS Microbiology Letters 33:153–157
    [Google Scholar]
  22. Williams P., Brown M.R.W., Lambert P.A. 1984; Effect of iron deprivation on the production of siderophores and outer membrane proteins inKlebsiella aerogenes . Journal of General Microbiology 130:2357–2365
    [Google Scholar]
/content/journal/micro/10.1099/00221287-135-2-445
Loading
/content/journal/micro/10.1099/00221287-135-2-445
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error