1887

Abstract

Comparisons of 23 virulent phages of subsp. were made based on morphology, host-range, structural protein analysis. DNA restriction patterns and DNA homology. All the phages had isometric heads (diameters 55–58 nm) and striated tails (lengths 221–275 nm). The genome sizes ranged from 37 to 43 kb. The electrophoretic profiles of the structural proteins were related, with at least two major bands of about 23 and 29 kDa in common for all the phages except three, 𝜙80, 𝜙96 and 𝜙99. These latter phages, which had a particular protein composition (main proteins of 29 and 40 kDa) and high DNA homology between each other, showed only a low DNA homology with the other phages. Extensive DNA homologies were demonstrated by Southern hybridization for all the other phages. All these results suggest a close evolutionary relationship between the phages.

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1989-12-01
2021-10-19
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References

  1. Accolas J. P., Spillman H. 1979; Morphology of six bacteriophages of Streptococcus thermophilus.. Journal of Applied Bacteriology 47:135–144
    [Google Scholar]
  2. Benbadis L., Faelen M., Castellino I., Fazel A., Mercenier A., Slos P. 1987; Phages of Streptococcus thermophilus.. FEMS Microbiology Reviews 46: (Abstracts) P43
    [Google Scholar]
  3. Botstein D. 1980; A theory of modular evolution for bacteriophages.. Annals of the New York Academy of Sciences 354:484–491
    [Google Scholar]
  4. Bradley D. E. 1967; Ultrastructure of bacteriophages and bacteriocins.. Bacteriological Reviews 31:230–314
    [Google Scholar]
  5. Coveney J. A., Fitzgerald G. F., Daly C. 1987; Detailed characterization and comparison of four lactic streptococcal bacteriophages based on morphology, restriction mapping, DNA homology, and structural protein analysis.. Applied and Environmental Microbiology 53:1439–1447
    [Google Scholar]
  6. Daly C., Fitzgerald G. 1987; Mechanisms of bacteriophage insensitivity in lactic streptococci.. In Streptococcal Genetics pp. 259–268 Ferretti J. J., Curtiss R. III Edited by Washington, DC: American Society for Microbiology;
    [Google Scholar]
  7. Denhardt D. T. 1966; A membrane filter technique for detection of complementary DNA.. Biochemical and Biophysical Research Communications 23:641–646
    [Google Scholar]
  8. Jarvis A. W. 1984; Differentiation of lactic streptococcal phages into phage species by DNA-DNA homology.. Applied and Environmental Microbiology 47:343–349
    [Google Scholar]
  9. Kivi S., PeltomÄki T., Sarimo S. S. 1987; Some properties of Streptococcus thermophilusbacterio-phages.. Folia microbiologica 32:101–106
    [Google Scholar]
  10. Klaenhammer T. R. 1987; Plasmid-directed mechanisms for bacteriophage defense in lactic streptococci.. FEMS Microbiology Reviews 46:313–325
    [Google Scholar]
  11. Laemmli U. K. 1970; Cleavage of structural proteins during assembly of the head of bacteriophage T4.. Nature; London: 227680–685
    [Google Scholar]
  12. Lahbib-Mansais Y., Mata M., Ritzenthaler P. 1988; Molecular taxonomy of Lactobacillusphages.. Biochimie 70:429–435
    [Google Scholar]
  13. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  14. Mata M., Ritzenthaler P. 1988; Present state of lactic acid bacteriophage taxonomy.. Biochimie 70:395–399
    [Google Scholar]
  15. Mata M., Trautwetter A., Luthaud G., Ritzenthaler P. 1986; Thirteen virulent and temperate bacteriophages of Lactobacillus bulgaricusand Lactobacillus lactisbelong to a single DNA homology group.. Applied and Environmental Microbiology 52:812–818
    [Google Scholar]
  16. Neve H., Krusch U., Teuber M. 1989; Classification of virulent bacteriophages of Streptococcus salivariussubsp. thermophilusisolated from yoghurt and Swiss-type cheese.. Applied Microbiology and Biotechnology 30:624–629
    [Google Scholar]
  17. Oakley B. R., Kirsch D. R., Morris N. R. 1980; A simplified ultrasensitive silver stain method for detecting proteins in acrylamide gels.. Analytical Biochemistry 105:361–363
    [Google Scholar]
  18. Reinbold G. W., Reddy M. S., Hammond E. G. 1982; Ultrastructure of bacteriophages active against Streptococcus thermophilus, Lactobacillus bulgaricus, Lactobacillus lactisand Lactobacillus helveticus.. Journal of Food Protection 45:119–124
    [Google Scholar]
  19. Relano P., Mata M., Bonneau M., Ritzenthaler P. 1987; Molecular characterization and comparison of 38 virulent and temperate bacteriophages of Streptococcus lactis.. Journal of General Microbiology 133:3053–3063
    [Google Scholar]
  20. Rigby P. W., Dieckmann M., Rhodes C., Berg P. 1977; Labelling deoxyribonucleic acid to high specific activity in vitroby nick translation with DNA polymerase I.. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  21. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis.. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  22. Terzaghi B. E., Sandine W. E. 1975; Improved media for lactic streptococci and their bacteriophages.. Applied Microbiology 29:807–813
    [Google Scholar]
  23. Teuber M., Krusch V., Luschei B., Neve H. 1987; Isolation and characterization of virulent bacteriophages of Streptococcus salivariussubsp. thermophilus.. FEMS Microbiology Reviews 46: (Abstracts) P42
    [Google Scholar]
  24. Trautwetter A., Ritzenthaler P., Alatossava T., Mata-Gilsinger M. 1986; Physical and genetic characterization of the genome of Lactobacillus lactis bacteriophage 1lh.. Journal of Virology 59:551–555
    [Google Scholar]
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