1887

Abstract

Several aspects of the adherence of purified elementary bodies (EB) of to HeLa and to McCoy cells were examined using different techniques, including an ELISA. Serovar-specific, biotinylated monoclonal antibodies were used to detect cell-bound chlamy-diae. In addition, purified chlamydiae were biotinylated and their adherence properties were studied. The assays were done at 4°C to exclude the energy-dependent internalization of the cell-bound EB and host-cell membrane recycling that occur at 37°C. Saturation kinetics were routinely observed at 4°C, and the rate of adherence remained linear for approximately 60 min. Lineweaver-Burk analysis of the kinetics data showed that adherence of any one serovar was competitively inhibited by other serovars of This competition for the same receptor on the two alternative hosts, HeLa and McCoy, was also seen when the adherence assays were done at 37°C in the presence of sodium azide, an energy poison that inhibits endocytosis of cell-bound chlamydiae. Chlamydiae exposed to 56°C for 5 min, or treated with low doses of trypsin, failed to exhibit competitive inhibition, having suffered considerable loss of the ability to adhere to host-cells. These data suggest that heat- and trypsin-labile chlamydial moieties participate in the adherence reaction, and that oculo-genital serovars of , including that of lymphogranuloma venereum, attach to the same receptor on the host-cell membrane.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-135-12-3229
1989-12-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/135/12/mic-135-12-3229.html?itemId=/content/journal/micro/10.1099/00221287-135-12-3229&mimeType=html&fmt=ahah

References

  1. Allan I., Pearce J. H. 1987; Association of Chlamydia trachomatis with mammalian and cultured insect cells lacking putative chlamydial receptors.. Microbial Pathogenesis 2:63–70
    [Google Scholar]
  2. Bose S. K., Goswami P. C. 1986a; Host modification of the adherence properties of Chlamydia trachomatis.. Journal of General Microbiology 132:1631–1639
    [Google Scholar]
  3. Bose S. K., Goswami P. C. 1986b; Chlamydial adherence to glycosylation-defective mutants of Chinese hamster cell lines.. In Chlamydial Infections pp. 31–34 Oriel D., Ridgway G., Schachter J., Taylor-Robinson D., Ward M. Edited by Cambridge: Cambridge University Press;
    [Google Scholar]
  4. Bose S. K., Paul R. G. 1982; Purification of Chlamydia trachomatis lymphogranuloma venereum elementary bodies and their interaction with HeLa cells.. Journal of General Microbiology 128:1371–1379
    [Google Scholar]
  5. Bose S. K., Smith G. B. 1984; Positive cooperativity in the adherence between elementary bodies of Chlamydia trachomatis strain UW-31 and HeLa cells.. FEMS Microbiology Letters 23:55–58
    [Google Scholar]
  6. Bose S. K., Smith G. B., Paul R. G. 1983; Influence of lectins, hexoses, and neuraminidase on the association of purified elementary bodies of Chlamydia trachomatis UW-31 with HeLa cells.. Infection and Immunity 40:1060–1067
    [Google Scholar]
  7. Byrne G. I. 1978; Kinetics of phagocytosis of Chlamydia psittaci to mouse fibroblasts (L cells): separation of the attachment and ingestion stages.. Infection and Immunity 19:607–612
    [Google Scholar]
  8. Byrne G. I., Moulder J. W. 1978; Parasite- specified phagocytosis of Chlamydia psittaci and Chlamydia trachomatis by L and HeLa cells.. Infection and Immunity 19:598–606
    [Google Scholar]
  9. Caldwell H. D., Perry L. J. 1982; Neutralization of Chlamydia trachomatis infectivity with antibodies to the major outer membrane protein.. Infection and Immunity 38:745–754
    [Google Scholar]
  10. Hackstadt T. 1986; Identification and properties of chlamydial polypeptides that bind eucaryotic cell surface components.. Journal of Bacteriology 165:13–20
    [Google Scholar]
  11. Hackstadt T., Caldwell H. D. 1985; Effect of proteolytic cleavage of surface-exposed proteins on infectivity of Chlamydia trachomatis.. Infection and Immunity 48:546–551
    [Google Scholar]
  12. Kuo C.-C., Wang S.-P., Grayston J. T. 1973; Effects of polycations, polyanions and neuraminidase on the infectivity of trachoma-inclusion conjunctivitis and lymphogranuloma venerum organisms in HeLa cells: sialic acid residues as possible receptors for trachoma-inclusion conjunctivitis.. Infection and Immunity 8:74–79
    [Google Scholar]
  13. Kuo C.-C., Grayston J. T. 1976; Interaction of Chlamydia trachomatis organisms and HeLa cells.. Infection and Immunity 13:1103–1109
    [Google Scholar]
  14. Lee C. K. 1981; Interaction between a trachoma strain of Chlamydia trachomatis and mouse fibroblasts (McCoy cells) in the absence of centrifugation.. Infection and Immunity 31:584–591
    [Google Scholar]
  15. Lucero M. E., Kuo C.-C. 1985; Neutralization of Chlamydia trachomatis cell culture infection by serovar-specific monoclonal antibodies.. Infection and Immunity 50:595–597
    [Google Scholar]
  16. Newhall W. J., Batteiger B., Jones R. B. 1982; Analysis of the human serological response to proteins of Chlamydia trachomatis.. Infection and Immunity 38:1181–1189
    [Google Scholar]
  17. Peeling R., Mclean I. W., Brunham R. C. 1984; In vitro neutralization of Chlamydia trachomatis with monoclonal antibody to an epitope on the outer membrane protein.. Infection and Immunity 46:484–488
    [Google Scholar]
  18. Ridderhof J., Barnes R. C. 1988; Enzyme immunoassay for measuring attachment of Chlamydia trachomatis to HeLa cells.. Annual Meeting of the American Society for Microbiology D-7, p. 72:
    [Google Scholar]
  19. Soderlund G., Kihlstrom E. 1983; Attachment and internalization of a Chlamydia trachomatis lymphogranuloma venereum strain by McCoy cells: kinetics of infectivity and effect of lectins and carbohydrates.. Infection and Immunity 42:930–935
    [Google Scholar]
  20. Su H., Zhang Y.-X., Barrera O., Watkins N. G., Caldwell H. D. 1988; Differential effect of trypsin on infectivity of Chlamydia trachomatis: loss of infectivity requires cleavage of major outer membrane protein variable domains II and IV.. Infection and Immunity 56:2094–2100
    [Google Scholar]
  21. Vretou E., Bezboruah R. L., Barnes R. C., Bose S. K. 1988a; Host modulation of chlamydial adherence.. Proceedings of the First Meeting of the European Society for Chlamydia Research p. 101 Bologna, Italy:: Societa Editrice Esculapio.;
    [Google Scholar]
  22. Vretou E., Goswami P. C., Bezboruah R. L., Bose S. K. 1988b; Host-controlled chlamydial adherence to human and murine cells. Annual Meeting of the American Society of Biochemistry and Molecular Biology.. Journal of Cell Biology 10 7:145a
    [Google Scholar]
  23. Wenman W. M., Meuser R. V. 1986; Chlamydia trachomatis elementary bodies possess proteins which bind to eucaryotic cell membranes.. Journal of Bacteriology 165:602–607
    [Google Scholar]
  24. Zhang Y.-X., Stewart S., Joseph T., Taylor H. R., Caldwell H. D. 1987; Protective monoclonal antibodies recognize epitopes located on the major outer membrane protein of Chlamydia trachomatis.. Journal of Immunology 138:575–581
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-135-12-3229
Loading
/content/journal/micro/10.1099/00221287-135-12-3229
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error