1887

Abstract

The total soluble proteins were extracted from 38 strains of pink-pigmented facultatively methylotrophic bacteria (PPFMs) and the similarity between them was investigated by polyacrylamide gel electrophoresis. Normalized densitometric recordings of the protein profiles were computer-compared by two fundamentally different modes: peak search and position analysis, and correlation coefficient analysis. Factors influencing the reproducibility of the system were investigated. The data indicated that the PPFMs were more heterogeneous than previously believed. The results from both analyses showed a high degree of congruence with each other and with data from previous studies.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-134-8-2375
1988-08-01
2021-10-20
Loading full text...

Full text loading...

/deliver/fulltext/micro/134/8/mic-134-8-2375.html?itemId=/content/journal/micro/10.1099/00221287-134-8-2375&mimeType=html&fmt=ahah

References

  1. Anthony C. 1982 The Biochemistry of Methylotrophs. London:: Academic Press.;
    [Google Scholar]
  2. Anthony C., Zatman L.J. 1964; Microbial oxidation of methanol: isolation and properties of Pseudomonas M27.. Biochemical Journal 92:609–614
    [Google Scholar]
  3. Austin B., Goodfellow M. 1979; Pseudomonas mesophilica, a new species of pink bacteria isolated from leaf surfaces.. International Journal of Systematic Bacteriology 29:373–378
    [Google Scholar]
  4. Chandra T.S., Shethna Y.I. 1975; Isolation and characterisation of some new oxalate decomposing bacteria.. Antonie van Leeuwenhoek 41:101–111
    [Google Scholar]
  5. Christiansen C., Black F.T., Freundt E.A. 1981; Hybridisation experiments with DNA from Ureaplasma urealyticumserovars I to VIII.. International Journal of Systematic Bacteriology 31:259–262
    [Google Scholar]
  6. Colby J., Zatman L.J. 1973; Trimethylamine metabolism in obligate and facultative methylotrophs.. Biochemical Journal 132:101–112
    [Google Scholar]
  7. Den Dooren De Jong L.E. 1927; Über protaminophage Bakterien.. Zentralblatt für Bakteriologie, Para-sitenkunde, Infektionskrankheiten und Hygiene 71:193–232
    [Google Scholar]
  8. Eckersley K., Dow C.S. 1980; Rhodopseudo-monas blastica sp. nov.: a member of the Rhodospirillaceae.. Journal of General Microbiology 119:465–473
    [Google Scholar]
  9. Feltham R.K.A., Sneath P.H.A. 1979; Quantitative comparison of electrophoretic traces of bacterial proteins.. Computers and Biomedical Research 12:247–263
    [Google Scholar]
  10. Graf W., Bauer L. 1973; Red bacterial growth in tap water systems.. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene 236:513–530
    [Google Scholar]
  11. Green P.N., Bousfield I.J. 1982; A taxonomic study of some Gram-negative facultativelymethylotrophic bacteria.. Journal of General Microbiology 128:623–638
    [Google Scholar]
  12. Green P.N., Bousfield I.J. 1983; Emendation of Methylobacterium Pattet al., 1976: Methylobacterium rhodos (Heumann, 1962): Methylobacterium radiora(Ito &Izuka, 1971): Methylobacterium mesophilica(Austin and Goodfellow, 1979).. International Journal of Systematic Bacteriology 33:875–877
    [Google Scholar]
  13. Hayward A.C. 1960; Relationship between Protaminobacter ruber and some red-pigmented pseudomonads.. Journal of Applied Bacteriology 23:II
    [Google Scholar]
  14. Heumann W. 1962; Die Methodik der Kreuzungsternbildener Bakterien.. Biologisches Zentralblatt 81:341–354
    [Google Scholar]
  15. Hood D.W., Dow C.S., Green P.N. 1987; DNA:DNA hybridization studies on the pink-pigmented facultative methylotrophs.. Journal of General Microbiology 133:709–720
    [Google Scholar]
  16. Ito H., Iizuka H. 1971; Taxonomic studies on a radio-resistant Pseudomonas, part XIII.. Agricultural and Biological Chemistry 35:1566–1571
    [Google Scholar]
  17. Jackman P.J.H. 1982; Classification of Corynebacterium species from axillary skin by numerical analysis of electrophoretic protein patterns.. Journal of Medical Microbiology 15:485–492
    [Google Scholar]
  18. Jackman P.J.H. 1985; Bacterial taxonomy based on electrophoretic whole cell protein patterns.. In Clinical Methods in Bacterial Systematics pp. 115–128 Goodfellow M., Minnikin D. Edited by London: Academic Press;
    [Google Scholar]
  19. Janota-Bassalik L., Pedyk D. 1961; Ability of Flavobacterium extorquens to utilise various sources of carbon with particular reference to glucose.. Acta microbiologica polonica 10:225–238
    [Google Scholar]
  20. Kersters K., De Ley J. 1975; Identification and grouping of bacteria by numerical analysis of their electrophoretic protein patterns.. Journal of General Microbiology 87:333–342
    [Google Scholar]
  21. Kersters K., De Ley J. 1980; Classification and identification of bacteria by electrophoresis of their proteins.. In Microbial Classification and Identification (SAB Symposium no. 8) p. 273–297 Goodfellow M., Board R. Edited by London: Academic Press;
    [Google Scholar]
  22. Kouno K., Ozaki A. 1975; Distribution and identification of methanol-utilising bacteria.. In Microbial Growth on C1 Compounds pp. 11–21 Tenui G. Others Edited by Japan: Society of Fermentation Technology;
    [Google Scholar]
  23. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4.. Nature; London: 227680–685
    [Google Scholar]
  24. Owen R.J., Jackman P.J.H. 1982; The similarities between Pseudomonas paucimobilis and allied bacteria derived from analysis of DNAs and electrophoretic protein patterns.. Journal of General Microbiology 128:2945–2954
    [Google Scholar]
  25. Patt T.E., Cole G.C., Hanson R.S. 1976; Methylobacterium, a new genus of facultatively methylotrophic bacteria.. International Journal of Systematic Bacteriology 26:226–229
    [Google Scholar]
  26. Peel D., Quayle J.R. 1961; Microbial growth on C1 compounds. Isolation and characterisation of Pseudomonas AM 1.. Biochemical Journal 81:465–469
    [Google Scholar]
  27. Rock J.S., Goldberg I., Ben-Bassat A., Mateles R.I. 1976; Isolation and characterisation of two methanol utilising bacteria.. Agricultural and Biological Chemistry 40:2129–2135
    [Google Scholar]
  28. Sneath P.H.A. 1978; Classification of microorganisms.. Essays in Microbiology 9:9/1–9/31
    [Google Scholar]
  29. Sneath P.H.A., Johnson R. 1972; The influence on numerical taxonomic similarities of errors in microbiological tests.. Journal of General Microbiology 72:377–392
    [Google Scholar]
  30. Sneath P.H.A., Sokal R.R. 1973 Numerical Taxonomy. San Francisco:: Freeman.;
    [Google Scholar]
  31. Studier F.W. 1973; Analysis of bactriophage T7 early RNAs and proteins in slab gels.. Journal of Molecular Biology 79:237–248
    [Google Scholar]
  32. Swings J., Kersters K., De Ley J. 1976; Numerical analysis of electrophoretic protein patterns of Zymomonas strains.. Journal of General Microbiology 93:266–271
    [Google Scholar]
  33. Urakami T., Komagata K. 1981; Electrophoretic comparison of enzymes in the Gram negative methanol utilising bacteria.. Journal of General and Applied Microbiology 27:381–403
    [Google Scholar]
  34. Whittenbury R., Dow C.S. 1977; Morphogenesis and differentiation in Rhodomicrobium vannieliiand other budding and prosthecate bacteria.. Bacteriological Reviews 41:754–808
    [Google Scholar]
  35. Wray W., Boulikas T., Wray V.P., Hancock R. 1981; Silver staining of proteins in polyacrylamide gels.. Analytical Biochemistry 118:197–203
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-134-8-2375
Loading
/content/journal/micro/10.1099/00221287-134-8-2375
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error