1887

Abstract

serotype secretes at least three kinds of glucosyltransferase with different enzymological and immunological properties. One of them is a primer-independent enzyme and seems to be the source of primer for the others, both of which are primer-dependent enzymes. Recently, we purified the primer-independent enzyme, the third glucosyltransferase in this group from strain AHT-k serotype In the present study, we examined the specificity of the antiserum against the primer-independent glucosyltransferase using extracellular culture-conditioned fluids of many strains of the various serotypes of The antiserum cross-reacted with the extracellular culture fluids from strains of serotypes and , in addition to serotype , but not with those of other serotypes, indicating that the primer-independent glucosyltransferase is secreted by the and , but not by and The antiserum did not completely inhibit the activity of the enzyme, even at more than twofold antibody excess, determined by indirect precipitation with immobilized staphylococcal protein A.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-134-5-1223
1988-05-01
2021-10-20
Loading full text...

Full text loading...

/deliver/fulltext/micro/134/5/mic-134-5-1223.html?itemId=/content/journal/micro/10.1099/00221287-134-5-1223&mimeType=html&fmt=ahah

References

  1. Beighton D., Russell R. R. B., Hayday H. 1981; The isolation and characterization of Streptococcus mutans serotype h from dental plaque of monkeys (Macaca fascicularis). Journal of General Microbiology 124:271–279
    [Google Scholar]
  2. Bratthall D. 1970; Demonstration of five serological groups of streptococcal strains resembling Streptococcus mutans. Odontologisk revy 21:143–152
    [Google Scholar]
  3. Coykendall A. L. 1977; Proposal to elevate the subspecies of Streptococcus mutans to species status, based on their molecular composition. International Journal of Systematic Bacteriology 27:26–30
    [Google Scholar]
  4. Critchley P., Wood J., Saxon C., Leach S. 1976; The polymerisation of dietary sugars by dental plaque. Caries Research 1:112–129
    [Google Scholar]
  5. Fukui K., Fukui Y., Moriyama Y. 1974; Some immunochemical properties of dextransucrase and invertase from Streptococcus mutans. Infection and Immunity 10:985–990
    [Google Scholar]
  6. Fukui K., Moriyama T., Miyake Y., Mizutani K., Tanaka O. 1981; Purification and properties of glucosyltransferase responsible for water-insoluble glucan synthesis from Streptococcus mutans. Infection and Immunity 37:1–9
    [Google Scholar]
  7. Fukui K., Kokeguchi S., Kato K., Miyake Y., Nogami R., Moriyama T. 1983; Immunochemical properties of glucosyltransferase from Streptococcus mutans. Infection and Immunity 39:762–766
    [Google Scholar]
  8. Gibbons R. J. 1968; Formation and significance of bacterial polysaccharides in caries ethiology. Caries Research 2:164–171
    [Google Scholar]
  9. Gibbons R. J., Banghart S. 1967; Synthesis of extracellular dextran by cariogenic bacteria and its presence in human dental plaque. Archives of Oral Biology 12:11–24
    [Google Scholar]
  10. Gibbons R. J., Nygaard M. 1968; Synthesis of insoluble dextran and its significance in the formation of gelatinous deposits by plaque-forming streptococci. Archives of Oral Biology 13:1249–1262
    [Google Scholar]
  11. Hanada N., Takehara T. 1987; Comparison of different water-soluble glucan synthases from Streptococcus mutans serotype g. Microbios 50:147–152
    [Google Scholar]
  12. Hanada N., Takehara T., Saeki E. 1987; Purification and characterization of a third glucosyltransferase from Streptococcus mutans serotype g. Journal of General Microbiology 133:1351–1358
    [Google Scholar]
  13. Kametaka S., Hayashi S., Miyake Y., Suginaka H. 1987; Electrophoretic studies of extracellular glucosyltransferases and fructosyltransferases from seventeen strains of Streptococcus mutans. Archives of Microbiology 147:207–212
    [Google Scholar]
  14. Kumada H., Umemoto T., Onisi M., Tsumori H., Shimamura A., Mukasa H. 1987; Purification and characterization of extracellular glucosyltransferase from Streptococcus mutans serotype b (subspecies rattus). Journal of General Microbiology 133:1435–1441
    [Google Scholar]
  15. Linzer R., Slade H. D. 1976; Characterization of an anti-glucosyltransferase serum specific for insoluble glucan synthesis by Streptococcus mutans. Infection and Immunity 13:494–500
    [Google Scholar]
  16. Mccabe M. M. 1985; Purification and characterization of a primer-independent glucosyltransferase from Streptococcus mutans 6715-13 mutant 27. Infection and Immunity 50:771–777
    [Google Scholar]
  17. Mukasa H. 1986; Properties of Streptococcus mutans glucosyltransferases. In Molecular Microbiology and Immunology of Streptococcus mutans pp. 121–132 Hamada S., Michalek S. M., Kiyono H., Menaker L., Mcghee J. R. Edited by Amsterdam, New York, Oxford: Elsevier;
    [Google Scholar]
  18. Ouchterlony O. 1958; Diffusion in gel methods for immunological analysis. Progress in Allergy 5:1–9
    [Google Scholar]
  19. Perch B., Kjems T., Ball C. 1974; Biochemical and serological properties of Streptococcus mutans from various human and animal sources. Actapathologica, microbiologica et immunologica scandinavica 82:357–370
    [Google Scholar]
  20. Shimamura A., Tsumori H., Mukasa H. 1982; Purification and properties of Streptococcus mutans extracellular glucosyltransferase. Biochimica et biophysica acta 702:72–80
    [Google Scholar]
  21. Shklair I. L., Keene H. J. 1974; A biochemical scheme for the separation of the five varieties of Streptococcus mutans. Archives of Oral Biology 19:1079–1081
    [Google Scholar]
  22. Takehara T., Hanada N., Saeki E. 1984; Interaction of glucosyltransferaseisozymes on glucan synthesis by Streptococcus mutans AHT (serotype g). Microbios Letters 27:113–120
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-134-5-1223
Loading
/content/journal/micro/10.1099/00221287-134-5-1223
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error