SUMMARY: We have cloned the Shv-1 β-lactamase gene from the R1010 plasmid into pACYC184. By subcloning and transposon mutagenesis we have localized the gene to a 1.6 kb I-I fragment of R1010, which is present in the recombinant plasmid pUB8. A 900 bp I fragment of pUB8 was shown to be a specific hybridization probe by testing against plasmids which encode 17 different β-lactamase enzymes. A comparison was made of the sensitivity of the Shv-1 probe labelled with either [S]dCTP or with photobiotin.


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