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When grown in alternating cycles of light and darkness, non-synchronous cultures of Gloeothece fixed N2 mainly in the dark phase. This diurnal pattern of N2 fixation was independent of the doubling time (69 ± 16 h) of the organism and, since cell division was asynchronous, N2 fixation does not seem to be confined to a specific phase in the cell cycle. Fluctuations in the rate of N2 fixation coincided with similar fluctuations in the rate of nitrogenase synthesis. Diurnal fluctuations also occurred in the utilization of glucan and acid-soluble polyphosphate and in the ADP/ATP ratio. Based on these observations, it is proposed that specific metabolic changes are involved in the regulation of the diurnal pattern of N2 fixation by Gloeothece.
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