1887

Abstract

Summary: A positive selection is described for isolating amidase-negative mutants from strains. The method is based on the conversion, via amidase activity, of glycollamide to glycollate which is growth inhibitory. Three types of mutant were isolated on lactate medium containing glycollamide: (i) mutants in which amidase activity was reduced or absent; (ii) double mutants that were amidase-negative and resistant to glycollate inhibition of growth; and (iii) glycollate-resistant mutants. By raising glycollamide concentrations in the selection medium, amidase-negative mutants were obtained from strains producing altered amidases with low specific acetamidase and glycollamidase activities. Glycollamide has wider applicability than fluoroacetamide as a selective agent for obtaining amidase-negative mutants.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-133-6-1527
1987-06-01
2019-10-14
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-133-6-1527
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error