1887

Abstract

Two bacterial strains excreting a yellow pigment were isolated from human urine and identified as . The pigment was produced in the late exponential and early stationary phases of growth. Minimal media supplemented with tyrosine, phenylalanine, 3,4-dihydroxyphenylacetate or tryptophan, as well as complex media, induced pigment production. UV-visible spectra of the extracted pigment had peaks characteristic of 2-hydroxy-5-carboxymethyl-muconate semialdehyde, produced from -cleavage of 3,4-dihydroxyphenylacetate by the enzyme 3,4-dihydroxyphenylacetate 2,3-dioxygenase (EC 1.13.11.15). This enzyme was active when the bacteria were grown under conditions promoting pigment production. The kinetics and factors affecting pigment production are also reported.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-133-3-773
1987-03-01
2022-01-20
Loading full text...

Full text loading...

/deliver/fulltext/micro/133/3/mic-133-3-773.html?itemId=/content/journal/micro/10.1099/00221287-133-3-773&mimeType=html&fmt=ahah

References

  1. Adachi K., Takeda Y., Senoh S., Rita H. 1964; Metabolism of p-hydroxyphenylacetic acid in Pseudomonas ovalis.. Biochimica et biophysica acta 93:483–493
    [Google Scholar]
  2. Ball A.P., Mcghie G., Geddes A.M. 1977; Serratia marcescens in a general hospital. Quarterly Journal of Medicine, New Series 46:63–71
    [Google Scholar]
  3. Blackley E, Halvorson H., Kurtz W. 1967; The piicrobial production and some characteristics of D-carboxymethyl-a-hydroxymuconic acid semialdehyde. Canadian Journal of Microbiology 13:159–165
    [Google Scholar]
  4. Blazevic D.J. 1980; Taxonomy, isolation and identification of Serratia.. In The Genus Serratia pp 3–13 Von Graevenitz A., Rubin S. J. Edited by Boca Raton: CRC Press;
    [Google Scholar]
  5. Clayton E., Von Graevenitz A. 1966; Nonpigmented Serratia marcescens.. Journal of the American Medical Association 197:1059–1064
    [Google Scholar]
  6. Cooper R.A., Skinner M.A. 1980; Catabolism of 3- and 4-hydfoxyphenylacetate by the 3,4-dihydroxy- phenylacetate pathway in Escherichia coli.. Journal of Bacteriology 143:302–306
    [Google Scholar]
  7. Cooper R.A., Jones D.C. 1985; Isolation afld mapping of Escherichia coli K12 mutants defective in phenylacetate degradation. Journal of General Microbiology 131:2753–2757
    [Google Scholar]
  8. Dauenhauer S.A., Hull R.A., Williams R.P. 1984; . Clotiing and expression in Escherichia coli of Serratia marcescens genes encoding prodigiosin biosynthesis. Journal of Bacteriology 158:1128–1132
    [Google Scholar]
  9. Farmer J.J. III Davis B.R., Hickman-Brenner F.W., Mcwhorter A., Huntley-Carter G.P., Asbury M.A., Biddle C., Wathew-Grady H.G., Elias C., Steigerwalt G.R., O’Hara C.M., Morris G.K., Smith P.B., Brenner D.J. 1985; Biochemical identification of new species and biogroiips of Enterobacteriaceae isolated from clinical specimens. Journal of Clinical Microbiology 21:46–76
    [Google Scholar]
  10. Grimont P.A.D., Grimont F. 1984; Genus VIII.Serratia.. In Bergey’s Manual of Systematic Bacteriology 1: pp 477–484 Krieg N. R. Edited by Baltimore: Williams & Wilkins;
    [Google Scholar]
  11. Holland S., Dale J.W. 1979; The effect of resistance plasmids on pigmentation of Serratia marcescens.. Microbios tetters 9:85–89
    [Google Scholar]
  12. Lannigan R., Bryan L.E. 1980; Increased frequency of acceptance of plasmidic deoxyribonucleic acid by non-pigmented strains of Serratia marcescens.. Microbios tetters 13:123–127
    [Google Scholar]
  13. Lorén J.G., Guinea J. 1978; Efecto de la concentracion de glucosa sobre la biosintesis de prodigiosina por Serratia marcescens.. Revista espahola de fisiologia 34:247–252
    [Google Scholar]
  14. Ming-Jerding, Williams R.P. 1983; Biosynthesis of prodigiosin by white strains of Serratia marcescens isolated from patients. Journal of Clinical Microbiology 17:476–480
    [Google Scholar]
  15. Ono-Kamimoto M. 1973; Studies on 3,4-dihydroxy- phenylacetate 2,3-dioxygenase. I. Role of iron, substrate binding, and some other properties. Journal of Biochemistry 74:1049–1059
    [Google Scholar]
  16. Platt D.J., Sommerville J.S. 1981; Pigmentation stability and the influence of RP4 on pigmentation in S. marcescens.. Society for General Microbiology Quarterly 8:100–101
    [Google Scholar]
  17. Purkayastha M., Williams R.P. 1960; Association of pigment with the cell envelope of Serratia marcescens.. Nature, tendon 187:349–350
    [Google Scholar]
  18. Sparnins V.L., Chapman P.J., Dagley S. 1974; Bacterial degradation of 4-hydroxyphenylacetic acid and homoprotocatechuatic acid. Journal of Bacteriology 120:159–167
    [Google Scholar]
  19. Torrey S. 1983; Prodigiosin by Serratia marcescens R2.Microbiological Synthesis.. Recent Advances pp 44–45 Torrey S. Edited by Park Ridge, New Jersey: Noyes Data Corp;
    [Google Scholar]
  20. Viñas M., Loren J.G., Guinea J. 1983; Particulate-bound pigment of Serratia marcescens and its association with cellular envelopes. Microbios tetters 24:19–26
    [Google Scholar]
  21. Williams R.P., Qadri S.M.H. 1980; The pigment of Serratia.. In The Genus Serratia pp Von Graevenitz A., Rubin S. J. Edited by Boca Raton: CRC Press;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-133-3-773
Loading
/content/journal/micro/10.1099/00221287-133-3-773
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error