1887

Abstract

The monobactam aztreonam and the cephalosporin ceftazidime, -lactam antibiotics that possess the same side chain R1, showed unusual effects on exponentially growing pneumococci compared to other -lactams. Both antibiotics did not induce lysis even at concentrations up to 2 mg ml, values well above the respective MICs. However, morphological alterations and growth inhibition of the cells were observed at much lower concentrations. Binding to penicillin-binding proteins (PBPs) could be monitored directly by using anti-aztreonam antiserum and the Western blot technique. Both antibiotics showed high affinity for PBP 3, but had an extremely low affinity for PBP 2b. It is suggested that the failure to bind to PBP 2b is responsible for the failure to induce lysis in pneumococci.

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/content/journal/micro/10.1099/00221287-133-3-755
1987-03-01
2024-04-26
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References

  1. Adkinson N.F., Swabb E.A., Sugerman A.A. 1984; Immunology of the monobactam aztreonam. Antimicrobial Agents and Chemotherapy 25:93–97
    [Google Scholar]
  2. Briese T., Hakenbeck R. 1983; Interaction between choline and the N-acetyl-muramyl-l-alan- ine amidase of Streptococcus pneumoniae.. In The Target of Penicillin pp. 173–178 Hakenbeck R., Hltje J.-V., Labischinski H. Edited by Berlin & New York: DeGruyter;
    [Google Scholar]
  3. Brown D.F.G., Reynolds P.E. 1980; Intrinsic resistance to beta-lactam antibiotics in Staphylococcus aureus. FEBS Letters 122:275–278
    [Google Scholar]
  4. Chase H.A., Reynolds P.E., Ward J.B. 1978; Purification and characterization of the penicillinbinding protein that is the lethal target of penicillin in Bacillus megater ium and Bacillus licheniformis.. European Journal of Biochemistry 88:275–285
    [Google Scholar]
  5. Fontana R., Canepari P., Satta G., Coyette J. 1980; Identification of the lethal target of benzylpenicillin in Streptococcus faecalis by in vivo penicillin binding studies. Nature; London: 28770–72
    [Google Scholar]
  6. Fontana R., Cerini R., Longoni P., Grossato A., Canepari P. 1983; Identification of a streptococcal penicillin-binding protein that reacts very slowly with penicillin. Journal of Bacteriology 155:1343–1350
    [Google Scholar]
  7. Frére J.-M., Joris B. 1985; Penicillin-sensitive enzymes in peptidoglycan biosynthesis. CRC Critical Reviews in Microbiology 11:299–396
    [Google Scholar]
  8. Hakenbeck R., Kohiyama M. 1982a; Labelling of pneumococcal penicillin-binding proteins with [3H]- propionylampicillin. FEMS Microbiology Letters 14:241–245
    [Google Scholar]
  9. Hakenbeck R., Kohiyama M. 1982b; Purification of penicillin-binding protein 3 from Streptococcus pneumoniae.. European Journal of Biochemistry 127:231–236
    [Google Scholar]
  10. Hakenbeck R., Tarpay M., Tomasz A. 1980; Multiple changes of penicillin-binding proteins in penicillin-resistant clinical isolates of Streptococcus pneumoniae.. Antimicrobial Agents and Chemotherapy 17:364–371
    [Google Scholar]
  11. Hakenbeck R., Briese T., Ellerbrok H. 1986; Antibodies against the benzylpenicilloyl-moiety as a probe for penicillin-binding proteins. European Journal of Biochemistry 157:101–106
    [Google Scholar]
  12. Lacks S., Hotchkiss R.D. 1960; A study of the genetic material determining an enzyme activity in Pneumococcus. Biochimica et biophysica acta 39:508–517
    [Google Scholar]
  13. Liu H.H., Tomasz A. 1985; Tolerance to penicillin in multiply drug-resistant natural isolates of Streptococcus pneumoniae.. Journal of Infectious Diseases 152:365–372
    [Google Scholar]
  14. Reynolds P.E., Shepherd S.T., Chase H.A. 1978; Identification of the binding protein which may be the target of penicillin action in Bacillus megaterium.. Nature; London: 271568–570
    [Google Scholar]
  15. Schwarz U., Seeger K., Wengenmayer F., Strecker H. 1981; Penicillin-binding proteins of Escherichia coli identified with a 125I-derivative of ampicillin. FEMS Microbiology Letters 10:107–109
    [Google Scholar]
  16. Spratt B.G. 1977; Properties of the penicillinbinding proteins of Escherichia coli K12. European Journal of Biochemistry 72:341–352
    [Google Scholar]
  17. Tomasz A. 1979; From penicillin-binding proteins to the lysis and death of bacteria: a 1979 view. Reviews of Infectious Diseases 1:434–467
    [Google Scholar]
  18. Tomasz A., Waks S. 1975; Mechanism of action of penicillin: triggering of the pneumococcal autolytic enzyme by inhibitors of cell wall synthesis. Proceedings of the National Academy of Sciences of the United States of America 72:4162–4166
    [Google Scholar]
  19. Tomasz A., Albino A., Zanati E. 1971; Multiple antibiotic resistance in a bacterium with suppressed autolytic system. Nature; London: 227138–140
    [Google Scholar]
  20. Williamson R., Tomasz A. 1980; Antibiotictolerant mutants of Streptococcus pneumoniae that are not deficient in autolytic activity. Journal of Bacteriology 144:105–113
    [Google Scholar]
  21. Williamson R., Tomasz A. 1985; Inhibition of cell wall synthesis and acylation of the penicillin binding proteins during prolonged exposure of growing Streptococcus pneumoniae to benzylpenicillin. European Journal of Biochemistry 151:475–483
    [Google Scholar]
  22. Williamson R., Hakenbeck R., Tomasz A. 1980; In vivo interaction of β-lactam antibiotics with the penicillin-binding proteins of Streptococcus pneumoniae. Antimicrobial Agents and Chemotherapy 18:629–637
    [Google Scholar]
  23. Zighelboim S., Tomasz A. 1980; Penicillinbinding proteins of multiply antibiotic-resistant South African strains of Streptococcus pneumoniae. Antimicrobial Agents and Chemotherapy 17:434–442
    [Google Scholar]
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