SUMMARY: ATCC 29487 (UASM 495) produces an outer-membrane-associated phosphatase and an excreted phosphatase. The cell-associated enzyme was compared to phosphatases of nine other Gram-negative gliding bacteria and to that of . The other three species of the genus also produce a particulate, cell-associated phosphatase. Antiserum prepared against the phosphatase from the outer membrane of effectively precipitated the phosphatases of two other strains and the enzymes of and . Some inhibition of the enzyme by the antiserum also was observed. No significant reaction could be detected beween the antiserum and the cell-associated phosphatases of species of and the excreted phosphatase of . The results indicate that the four species of the genus are closely related despite their physiological differences and that the outer-membrane-associated phosphatases of these organisms have different structural characteristics than the phosphatases of the other Gramnegative bacteria that were used. Furthermore, differences in the amino acid compositions of the cell-associated and the excreted phosphatase of confirm the immunological results and are in agreement with the physical and chemical differences noted between the two enzymes.


Article metrics loading...

Loading full text...

Full text loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error