1887

Abstract

SUMMARY: The wax esters of strains NCIB 8250 and NCIB 10487 harvested at stationary phase from N-limited batch cultures were extracted and shown to consist of C to C saturated and mono-unsaturated alkan-l-ols randomly esterified with C to C saturated and mono-unsaturated fatty acids. The mono-unsaturated components contained a Δ9 double bond. Wax ester content of strain NCIB 8250 increased under conditions of low growth rate in N-limited continuous culture with carbon and energy source in excess. The high content of wax ester in N-limited cultures of strain NCIB 8250 was lowered by incubation in the absence of a carbon and energy source and the wax ester was converted to water-soluble materials and CO. It is proposed that in NCIB 8250 the endogenous wax ester present in N-limited cells can serve as an energy reserve. All 19 strains of tested contained some wax ester and as 16 of these strains had increased wax ester contents when harvested from stationary phase N-limited batch cultures, it appears that wax esters are widespread, but not universal, energy storage components in the genus

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1986-11-01
2021-05-09
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References

  1. Aasen A. J., Hofstelter H. H., Iyengar B. T. R., Holman R. T. 1971; Identification and analysis of wax esters by mass spectrometry. Lipids 6:502–507
    [Google Scholar]
  2. Albro P. W. 1976; Bacterial waxes. In Chemistry and Biochemistry of Natural Waxes pp. 419–445 Edited by Kolattukudy P. E. Amsterdam: Elsevier;
    [Google Scholar]
  3. Baer E., Fisher H. O. L. 1945; Synthesis of a homologous series of optically active normal aliphatic α-monoglycerides (l series). Journal of the American Chemical Society 67:2031–2037
    [Google Scholar]
  4. Bartlett G. R. 1959; Phosphorus assay in column chromatography. Journal of Biological Chemistry 234:466–468
    [Google Scholar]
  5. Baumann P., Doudoroff M., Stanier R. Y. 1968; A study of the Moraxella group II. Oxidative-negative species (genus Acinetobacter). Journal of Bacteriology 95:1520–1541
    [Google Scholar]
  6. Beggs J. D., Fewson C. A. 1977; Regulation of synthesis of benzyl alcohol dehydrogenase in Acinetobacter calcoaceticus NCIB 8250. Journal of General Microbiology 103:127–140
    [Google Scholar]
  7. Bligh E. G., Dyer W. J. 1959; A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology 37:911–917
    [Google Scholar]
  8. Bryn K., Jantzen E. B., vre K. 1977; Occurrence and patterns of waxes in Neisseriaceae. Journal of General Microbiology 102:33–43
    [Google Scholar]
  9. Capella P., Zorzut C. M. 1968; Determination of double bond position in monounsaturated fatty acid esters by mass spectrometry of their trimethylsil-oxy derivatives. Analytical Chemistry 40:1458–1463
    [Google Scholar]
  10. Caroll K. K. 1976; Column chromatography of neutral glycerides and fatty acids. Lipid Chromatographic Analysis vol. 1 pp. 173–214 Edited by Marinetti. G. V. London: Edward Arnold;
    [Google Scholar]
  11. Christie W. W. 1975 Lipid Analysis, 2nd edn.. Oxford: Pergamon Press.;
    [Google Scholar]
  12. Christopher R. K., Duffield A. M., Ralph B. T. 1980; Identification of some neutral lipids of Thiobacillus thioparus using gas chromatographychemical ionisation mass spectrometry. Australian Journal of Biological Science 33:737–744
    [Google Scholar]
  13. Clifton C. E. 1937; On the possibility of preventing assimilation in respiring cells. Enzymologia 4:246–253
    [Google Scholar]
  14. Dawes E. A., Ribbons D. W. 1964; Some aspects of the endogenous metabolism of bacteria. Bacteriological Reviews 28:126–149
    [Google Scholar]
  15. Dawes E. A., Senior P. J. 1973; The role and regulation of energy reserve polymers in microorganisms. Advances in Microbial Physiology 10:135–297
    [Google Scholar]
  16. Fewson C. A. 1967; The growth and metabolic versatility of the Gram-negative bacterium NCIB 8250 (‘Vibrio 01’). Journal of General Microbiology 46:255–266
    [Google Scholar]
  17. Finnerty W. R., Kallio R. E. 1964; Origin of palmitic acid carbon in palmitate formed from hexadecane-l-14C and tetradecane-l-I4C by Micrococcus cerificans. Journal of Bacteriology 87:1261–1265
    [Google Scholar]
  18. Fixter L. M., Fewson C. A. 1974; The accumulation of waxes by Acinetobacter calcoaceticus NCIB 8250. Biochemical Society Transactions 2:944–945
    [Google Scholar]
  19. Fixter L. M., McCormack J. G. 1976; The effect of growth conditions on the wax content of various strains of Acinetobacter. Biochemical Society Transactions 4:504–505
    [Google Scholar]
  20. Folch J., Lees M., Sloane-Stanley G. H. 1957; A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry 226:497–509
    [Google Scholar]
  21. Gallagher I. H. C. 1971; Occurrence of waxes in Acinetobacter. Journal of General Microbiology 68:245–247
    [Google Scholar]
  22. Harvey N. L., Fewson C. A., Holms W. H. 1968; Apparatus for batch culture of microorganisms. Laboratory Practice 17:1134–1136
    [Google Scholar]
  23. Hobbs G., Hardy R., Mackie P. 1971; Characterization of Clostridium species by means of their lipids. Journal of General Microbiology 68:ii–iii
    [Google Scholar]
  24. Kates M. 1975 Techniques of Lipidology; Isolation, Analysis and Identification of Lipids New York: American Elsevier.;
    [Google Scholar]
  25. Kates M., Adams G. A., Martin S. M. 1964; Lipids of Serratia marcescens. Canadian Journal of Biochemistry 42:461–479
    [Google Scholar]
  26. Klug M. J., Markovetz A. J. 1971; Utilisation of aliphatic hydrocarbons by microorganisms. Advances in Microbial Physiology 5:1–43
    [Google Scholar]
  27. Naccarato W. F., Gilbertson J. R., Gelman R. A. 1972; Characterisation and metabolism of free fatty alcohols from Escherichia coli. Lipids 7:275–281
    [Google Scholar]
  28. Nishimura Y., Yamamoto H., Iizuka H. 1979; Taxonomical studies of Acinetobacter species-cellular fatty acid composition. Zeitschrift fur Allgemeine Mikrobiologie 19:307–308
    [Google Scholar]
  29. Russell N. J. 1974; The lipid composition of the psychrophilic bacterium Micrococcus cryophilus. Journal of General Microbiology 80:217–225
    [Google Scholar]
  30. Russell N. J., Volkman J. K. 1980; The effect of growth temperature on wax ester composition in the psychrophilic bacterium Micrococcus cryophilus ATCC 15174. Journal of General Microbiology 118:131–141
    [Google Scholar]
  31. Sellinger Z., Lapidot Y. 1966; Synthesis of fatty acid anhydrides by reaction with dicyclohexylcarbo-diimide. Journal of Lipid Research 7:174–175
    [Google Scholar]
  32. Skipski V. P., Barclay M. 1969; Thin layer chromatography of lipids. Methods in Enzymology 14:530–598
    [Google Scholar]
  33. Sugimoto C., Miyagawa E., Nakazawa M., Mi-tani K., Isayama Y. 1983; Cellular fatty acid composition of Haemophilus equigenitalis and Moraxella species. International Journal of Systematic Bacteriology 33:181–187
    [Google Scholar]
  34. Vorbeck M. L., Marinetti G. V. 1965; Separation of glycosyl diglycerides from phosphatides using silicic acid column chromatography. Journal of Lipid Research 6:3–6
    [Google Scholar]
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