SUMMARY: Several regression methods were tested for estimating the sizes of a wide range of plasmids (1·37–312 MDa) and restriction fragments (2·2–14·2 MDa) by agarose gel electrophoresis. The most accurate and least variable method was the multiple regression of log molecular size against log relative mobility and the reciprocal square root of the relative mobility. This method gave a good fit to all the data with low percentage errors of the molecular size estimates (≤ 3·0 ± 1·5%). It is suggested that with this method the molecular size of unknown plasmids can be accurately estimated using the plasmids from V517 and IR713 as standards.


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