1887

Abstract

Summary: The mycolic and fatty acids of three samples each of and were compared. Acids released by whole-organism alkaline hydrolysis were converted to 4-nitrobenzyl esters and mycolic acids were further derivatized to -butyldimethylsilyl ethers. Thin-layer chromatography of the derivatized long-chain extracts showed that all three preparations contained so-called α-mycolates and ketomycolates but that the samples had a methoxymycolate in addition to the above types. Silica gel normal-phase high-performance liquid chromatography of the total mycolic acid derivatives confirmed the lack of detectable amounts of methoxymycolates in and reverse-phase chromatography of the individual mycolate types demonstrated the homogeneity of the chain lengths of the mycolic acids in each species. Non-hydroxylated fatty acid 4-nitrobenzyl esters were transformed to methyl esters and examined by gas chromatography. Tuberculostearic (10-methyloctadecanoic) acid was a major component of the lipids of all three preparations but it was absent in one strain and a very minor component in the other representatives of this latter species. On the basis of fatty and mycolic acid compositions, therefore, a previously suggested close relationship between and was not supported.

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1985-08-01
2022-01-17
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References

  1. Alvin C., Larsson L., Magnusson M., Mårdh P. -A., Odham G., Westerdahl G. 1983; Determination of fatty acids and carbohydrate monomers in micro-organisms by means of glass capillary gas chromatography: analysis of Mycobacterium gordonae and Mycobacterium scrofulaceum . Journal of General Microbiology 129:401–405
    [Google Scholar]
  2. Andersen O., Jantzen E., Closs O., Harboe M., Saxegaard F., Fodstad F. 1982; Fatty acid and polar lipid analysis as tools in the identification of Mycobacterium leprae and some related slow-growing mycobacterial species. Annales de Microbiologie 133B:29–37
    [Google Scholar]
  3. Asselineau C., Clavel S., Clément F., Daffé M., David H., Lanéelle M. A., Promé J. C. 1981; Constituants lipidiques de Mycobacterium leprae isolé de tatou infecté expérimentalement. Annales de Microbiologie 132A:19–30
    [Google Scholar]
  4. Convit J., Aranzazu N., Ulrich M., Pinardi M. E., Reyes O., Alvarado J. 1982; Immunotherapy with a mixture of Mycobacterium leprae and BCG in different forms of leprosy and in Mitsuda-negative contacts. International Journal of Leprosy 50:415–424
    [Google Scholar]
  5. Daffé M., Lanéelle M. A., Promé D., Asselineau C. 1981; Étude des lipides de Mycobacterium gordonae comparativement à ceux de M. leprae et de quelques mycobactéries scotochromogenes. Annales de Microbiologie 132B:3–12
    [Google Scholar]
  6. Daffé M., Lanéelle M. A., Asselineau C., Lévy-Frébault V., David H. 1983; Intérêt taxonomique des acides gras des mycobactéries: proposition d'une méthode d'analyse. Annales de Microbiologie 134B:241–256
    [Google Scholar]
  7. Dobson G., Minnikin D. E., Minnikin S. M., Parlett J. H., Goodfellow M., Ridell M., Magnusson M. 1985; Systematic analysis of complex mycobacterial lipids. In Chemical Methods in Bacterial Systematics pp 237–265 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  8. Draper P. 1976; Cell walls of Mycobacterium leprae . International Journal of Leprosy 44:95–98
    [Google Scholar]
  9. Draper P., Dobson G., Minnikin D. E., Minnikin S. M. 1982; The mycolic acids of Mycobacterium leprae harvested from experimentally infected nine-banded armadillos. Annales de Microbiologie 133B:39–47
    [Google Scholar]
  10. Draper P., Payne S. N., Dobson G., Minnikin D. E. 1983; Isolation of a characteristic phthiocerol dimycocerosate from Mycobacterium leprae . Journal of General Microbiology 129:859–863
    [Google Scholar]
  11. Etémadi A. H., Convit J. 1974; Mycolic acids from ‘noncultivable’ mycobacteria. Infection and Immunity 10:235–239
    [Google Scholar]
  12. Hunter S. W., Brennan P. J. 1983; Further specific extracellular phenolic glycolipid antigens and a related diacylphthiocerol from Mycobacterium leprae . Journal of Biological Chemistry 258:7556–7562
    [Google Scholar]
  13. Julák J., Tureček F., Miková Z. 1980; Identification of characteristic branched-chain fatty acids of Mycobacterium kansasii and gordonae by gas chromatography–mass spectrometry. Journal of Chromatography 190:183–187
    [Google Scholar]
  14. Kusaka T., Izumi S. 1983; Gas chromatography of constitutive fatty acids in Mycobacterium leprae . Microbiology and Immunology 27:409–414
    [Google Scholar]
  15. Kusaka T., Kohsaka K., Fukunishi Y., Akimori G. 1982; Isolation and identification of mycolic acids in Mycobacterium leprae and Mycobacterium lepraemurium . International Journal of Leprosy 49:406–416
    [Google Scholar]
  16. Minnikin D. E., Hutchinson I. G., Caldicott A. B., Goodfellow M. 1980; Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. Journal of Chromatography 188:221–233
    [Google Scholar]
  17. Minnikin D. E., Minnikin S. M., Goodfellow M. 1982; The oxygenated mycolic acids of Mycobacterium fortuitum, M. farcinogenes and M. senegalense . Biochimica et biophysica acta 712:616–620
    [Google Scholar]
  18. Minnikin D. E., Dobson G., Hutchinson I. G. 1983a; Characterization of phthiocerol dimycocerosates from Mycobacterium tuberculosis . Biochimica et biophysica acta 753:445–449
    [Google Scholar]
  19. Minnikin D. E., Minnikin S. M., Dobson G., Goodfellow M., Portaels F., van den Breen L., Sesardic D. 1983b; Mycolic acid patterns of four vaccine strains of Mycobacterium bovis BCG. Journal of General Microbiology 129:889–891
    [Google Scholar]
  20. Minnikin D. E., Minnikin S. M., O'Donnell A. G., Goodfellow M. 1984a; Extraction of mycobacterial mycolic acids and other long-chain compounds by an alkaline methanolysis procedure. Journal of Microbiological Methods 2:243–249
    [Google Scholar]
  21. Minnikin D. E., Minnikin S. M., Parlett J. H., Goodfellow M., Magnusson M. 1984b; Mycolic acid patterns of some species of Mycobacterium . Archives of Microbiology 139:225–231
    [Google Scholar]
  22. Minnikin D. E., Parlett J. H., Magnusson M., Ridell M., Lind A. 1984c; Mycolic acid patterns of representatives of Mycobacterium bovis BCG. Journal of General Microbiology 130:2733–2736
    [Google Scholar]
  23. Minnikin D. E., Dobson G., Draper P. 1985; The free lipids of Mycobacterium leprae harvested from experimentally infected nine-banded armadillos. Journal of General Microbiology 131:2007–2011
    [Google Scholar]
  24. Miwa T. K., Mikolajczak K. L., Earle F. R., Wolff I. A. 1960; Gas chromatographic characterization of fatty acids. Analytical Chemistry 32:1739–1742
    [Google Scholar]
  25. Shepard C. C., Draper P., Rees R. J. W., Lowe C. 1980; Effect of purification steps on the immunogenicity of Mycobacterium leprae . British Journal of Experimental Pathology 61:376–379
    [Google Scholar]
  26. Shepard C. C., van Landingham R. M., Walker L. L., Ye S. -Z. 1983; Comparison of the immunogenicity of vaccines prepared from viable Mycobacterium bovis BCG, heat killed Mycobacterium leprae, and a mixture of the two for normal and M. leprae-tolerant mice. Infection and Immunity 40:1096–1103
    [Google Scholar]
  27. Tisdall P. A., Roberts G. D., Anhalt J. P. 1979; Identification of clinical isolates of mycobacteria with gas–liquid chromatography alone. Journal of Clinical Microbiology 10:506–514
    [Google Scholar]
  28. World Health Organization 1980; UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases. Report of the Fifth Meeting of the Scientific Working Group on the Immunology of Leprosy (IMMLEP). TDR/IMM-LEP-SWG(5)/80.3, Annex 4, p 23 Geneva: World Health Organization;
    [Google Scholar]
  29. Young D. B. 1980; Identification of Mycobacterium leprae: use of wall-bound mycolic acids.. Journal of General Microbiology 121:249–253
    [Google Scholar]
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