1887

Abstract

The fission yeast , grown in the presence of aculeacin A (Acu), an antifungal antibiotic, forms spherical rather than cylindrical walls. This aberrant morphogenesis was studied in the presence of Acu at 1 μg ml under conditions in which over 90% of the cells continued to grow but with altered morphology. Microscopic observations and chemical analyses revealed that the spherical walls had a looser structure than the cylindrical wall and that their syntheses of alkali-insoluble glucan and mannan were reduced whereas the synthesis of alkali-soluble glucan was enhanced. Spherical walls of glutaraldehyde-fixed cells were hardly digested by Zymolyase 60000 [a (1→3)--glucanase] but were digested by Novozym 234 [a (1→3)--glucanase]. From these results the components and morphology of the spherical walls are discussed in comparison with the cylindrical walls.

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/content/journal/micro/10.1099/00221287-131-3-611
1985-03-01
2021-07-28
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