1887

Abstract

We have determined a sequence of 2073 bp from two recombinant plasmids carrying the whole locus from , the expression of which is required for spore formation. The sequence contains three long open reading frames (ORFs), each of them being preceded by a ribosome binding site. These three putative proteins (mol. wts 13100, 16300 and 22200) are likely to be expressed and are probably encoded on the same mRNA. The stop codon of ORF overlaps with the start codon of ORF2 suggesting that there might be translational coupling between the two ORFs. Although some known promoter sequences were found, the only one upstream from the first open reading frame is about 260 bp from it.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-130-8-2147
1984-08-01
2021-10-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/130/8/mic-130-8-2147.html?itemId=/content/journal/micro/10.1099/00221287-130-8-2147&mimeType=html&fmt=ahah

References

  1. Akrigg A., Mandelstam J. 1978; Extracellular manganese-stimulated deoxyribonuclease produced during sporulation of Bacillus subtilis. Biochemical Journal 172:69–76
    [Google Scholar]
  2. Biggin M. D., Gibson T. J., Hong G. F. 1983; Buffer gradient gel and 35S-label as an aid to rapid DNA sequence determination. Proceedings of the National Academy of Sciences of the United States of America 80:3963–3965
    [Google Scholar]
  3. Birnboim H. C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  4. Chak K. F., Lencastre H.DE, Liu H. M., Piggot P. J. 1982; Facile in vivotransfer of mutations between the Bacillus subtilischromosome and a plasmid harbouring homologous DNA. Journal of General Microbiology 128:2813–2816
    [Google Scholar]
  5. Coote J. G. 1972a; Sporulation in Bacillus subtilis.Characterization of oligosporogenous mutants and comparison of their phenotypes with those of asporo- genous mutants. Journal of General Microbiology 71:1–15
    [Google Scholar]
  6. Coote J. G. 1972b; Sporulation in Bacillus subtilis.Genetic analysis of oligosporogenous mutants. Journal of General Microbiology 71:17–27
    [Google Scholar]
  7. Doolittle R. F. 1981; Similar amino acid sequences. Chance or common ancestry?. Science 2124:149–159
    [Google Scholar]
  8. Errington J., Mandelstam J. 1983; Variety of sporulation phenotypes resulting from mutations in a single regulatory locus, spoIIA,in Bacillus subtilis. Journal of General Microbiology 129:2091–2101
    [Google Scholar]
  9. Errington J., Mandelstam J. 1984; Genetic and phenotypic characterization of a cluster of mutations in the spoVAlocus of Bacillus subtilis. Journal of General Microbiology 130:2115–2121
    [Google Scholar]
  10. Guo L. H., Yang R. C. A, Wu R. 1983; An improved strategy for rapid direct sequencing of both strands of long DNA molecules cloned in a plasmid. Nucleic Acids Research 11:5521–5540
    [Google Scholar]
  11. Haseltine W. A., Lindan C. P., D’Andrea A. D., Johnsrud L. 1980; The use of DNA fragments of defined sequence for the study of DNA damage and repair. Methods in Enzymology 65:235–248
    [Google Scholar]
  12. Ionesco H., Schaeffer P. 1968; Localisationchromosomique de certains mutants asporogenes de Bacillus subtilisMarburg. Annales de l’Institut Pasteur 114:11–20
    [Google Scholar]
  13. Jeffreys A. J., Flavell R. A. 1977; A physical map of the DNA regions flanking the rabbit β-globin gene. Cell 12:429–439
    [Google Scholar]
  14. Johnson W. C., Moran C. P.Jr Losick R. 1983; Two RNA polymerase sigma factors from Bacillus subtilisdiscriminate between overlapping promoters for a developmentally regulated gene. Nature; London: 302800–804
    [Google Scholar]
  15. Lencastre H.DE, Chak K. F., Piggot P. J. 1983; Use of the Escherichia colitransposon Tn1000 (γδ)to generate mutations in Bacillus subtilisDNA. Journal of General Microbiology 129:3203–3210
    [Google Scholar]
  16. Liu H. M., Chak K. F., Piggot P. J. 1982; Isolation and characterization of a recombinant plasmid carrying a functional part of the Bacillus subtilisspoIIAlocus. Journal of General Microbiology 128:2805–2812
    [Google Scholar]
  17. Mclaughlin S. R., Murray C. L., Rabinowitz J. C. 1981; Unique features in the ribosome binding site sequence of the Gram-positive Staphylococcus aureus β-lactamase gene. Journal of Biological Chemistry 256:11283–11291
    [Google Scholar]
  18. Mcmaster G. K., Samulski R. S., Stein J. L., Stein G. S. 1980; Rapid purification of covalently closed circular DNAs of bacterial plasmids and animal tumour viruses. Analytical Biochemistry 109:47–54
    [Google Scholar]
  19. Messing J., Vieira J. 1982; A new pair of M13 vectors for selecting either DNA strand of doubledigest restriction fragments. Gene 19:269–276
    [Google Scholar]
  20. Moran C. P.Jr Lang N., Losick R. 1981; Nucleotide sequence of a Bacillus subtilispromoter recognized by Bacillus subtilisRNA polymerase containing σ37. Nucleic Acids Research 9:5979–5990
    [Google Scholar]
  21. Moran C. P.Jr Johnson W. C., Losick R. 1982a; Close contacts between σ37-RNA polymerase and a Bacillus subtilischromosomal promoter. Journal of Molecular Biology 162:709–713
    [Google Scholar]
  22. Moran C. P.Jr Lang N., Grice S. F. J, Lee G., Stephens M., Sonenshein A. L., Pero J., Losick R. 1982; b. Nucleotide sequences that signal the initiation of transcription and translation in Bacillus subtilis. Molecular and General Genetics 186:339–346
    [Google Scholar]
  23. Oppenheim D. S., Yanofsky C. 1980; Translational coupling during expression of the tryptophan operon of Escherichia coli. Genetics 95:785–795
    [Google Scholar]
  24. Piggot P. J. 1973; Mapping of asporogenous mutations of Bacillus subtilis:a minimum estimate of the number of sporulation operons. Journal of Bacteriology 114:1241–1253
    [Google Scholar]
  25. Piggot P. J., Coote J. G. 1976; Genetic aspects of bacterial endospore formation. Bacteriological Reviews 40:908–962
    [Google Scholar]
  26. Piggot P. J., Moir A., Smith D. A. 1981; Advances in the genetics of Bacillus subtilisdifferentiation. In Spores VIII pp. 29–39 Levinson H., Tipper D., Sonenshein A. L. Edited by Washington, D.C. :: American Society for Microbiology.;
    [Google Scholar]
  27. Piggot P. J., Chak K. F., Liu H. M., Lencastre H.DE. 1983; Bacillus subtilis:genetics and spore formation. In Microbiology-1983 pp. 163–166 Schlessinger D. Edited by Washington, DC:: American Society for Microbiology;
    [Google Scholar]
  28. Piggot P. J., Curtis C. A. M, Lencastre H.DE. 1984; Use of integrational plasmid vectors to demonstrate the polycistronic nature of a transcriptional unit (spoilA)required for sporulation of Bacillus subtilis. Journal of General Microbiology 130:2123–2136
    [Google Scholar]
  29. Rigby P. W. J, Dieckmann M., Rhodes C., Berg P. 1977; Labelling deoxyribonucleic acid to high specific activity in vitroby nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  30. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain terminating inhibitors. Proceedings of the National Academy of Sciences of the United States of America 74:5463–5468
    [Google Scholar]
  31. Sanger F., Coulson A. R., Darnell B. G., Smith A. J., Roc B. A. 1980; Cloning in single stranded bacteriophage as a aid to rapid DNA sequencing. Journal of Molecular Biology 143:161–178
    [Google Scholar]
  32. Sanger F., Coulson A. R., Hong G. F., Hill D. F., Petersen G. B. 1982; Nucleotide sequence of bacteriophage λDNA. Journal of Molecular Biology 162:729–773
    [Google Scholar]
  33. Savva D., Mandelstam J. 1984; Cloning of the Bacillus subtilis spoil Aand spoVAloci in phage ø105DI:lt. Journal of General Microbiology 130:2137–2145
    [Google Scholar]
  34. Shine J., Dalgarno L. 1974; The 3' terminal sequence of Escherichia coli16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites. Proceedings of the National Academy of Sciences of the United States of America 71:1342–1346
    [Google Scholar]
  35. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  36. Staden R. 1977; Sequence data handling by computer. Nucleic Acids Research 4:4037–4051
    [Google Scholar]
  37. Tinoco I., Borer P., Dengler B., Levine M., Uhlenbeck O., Crothers D., Gralla J. 1973; Improved estimation of secondary structure in ribonucleic acids. Nature, New Biology 246:40–41
    [Google Scholar]
  38. Young M., Mandelstam J. 1979; Early events during bacterial endospore formation. Adrances in Microbial Physiology 20:103–162
    [Google Scholar]
  39. Yudkin M. D., Turley L. 1980; Suppression of asporogeny in Bacillus subtilis.Allele-specific suppression of a mutation in the spoIIAlocus. Journal of General Microbiology 121:69–78
    [Google Scholar]
  40. Yudkin M. D., Turley L. 1981; Mapping of six mutations in the spoIIA locus of Bacillus subtilis and studies of their response to a nonsense suppressor. Journal of General Microbiology 124:255–261
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-130-8-2147
Loading
/content/journal/micro/10.1099/00221287-130-8-2147
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error