Summary: DNA preparations from two reference (H37Ra and H37Rv) and two wild strains of and one re-isolated strain of BCG were analysed using 17 restriction endonucleases. The enzyme EII revealed the greatest differences between strains. Electrophoretic DNA patterns from the wild strains differed from each other and from the reference strains at relatively few positions. At the highest resolution attained, patterns from the two reference strains remained indistinguishable from each other. The pattern of the BCG strain was substantially different from, but had many bands in common with, the patterns.


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