SUMMARY: CW977 produced high yields of the bacteriocin proticine 3 upon mitomycin C induction of cultures growing at 30 °C. The proticine was purified and found to have a relative density 1-299 and to be composed of 10 proteins assembled into structures resembling contractile phage tails. When induction was performed at 41 °C neither proticine particles nor proticine activity was detected, although the growth rate of cells and degree of lysis were indistinguishable from that at 30 °C. Failure in proticine production was due to a 41 °C sensitive stage occurring between 60 and 90 min after the addition of mitomycin C. During this period at 30 °C, two proteins of mol. wt 58000 and 41000 were formed. These proteins were associated with events leading to the formation of proticine particles with biological activity. When the production of both proteins was prevented either by chloramphenicol or as a result of mutation or through sampling before they were formed, no proticine particles were found nor proticine activity detected. The synthesis of both proteins was also inhibited at 41 °C. Co-electrophoresis of the labelled proteins with unlabelled purified proticine confirmed that the protein of mol. wt 58000 was a proticine structural protein. The protein of mol. wt 41000 was not a structural component of proticine and its role, if any, in proticine 3 production is possibly that of an assembly protein.


Article metrics loading...

Loading full text...

Full text loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error