Summary: Fifty-six strains of non-lactose fermenting coliform organisms belonging to the Providence group were obtained from the National Collection of Type Cultures and examined for their nutritional requirements. Fifty-five of these strains formed a nutritionally homogeneous group. The simplest medium supporting growth was a basal salt medium with added glucose, glutamic acid and cystine. Pantothenate usually improved growth and appeared to be nearly essential for two strains. The remaining strain possessed the nutritional pattern of and in this and other characters appeared to be a typical strain of that species. Twenty-two of the fifty-five strains gave a positive amine test using the ninhydrin test-paper technique; previously only species in the family Enterobacteriaceae had been observed to produce amines. The classification of the Providence group is discussed in the light of these findings.


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