1887

Microbiology Society logo

Volume 129, Issue 9

Growth Temperature-dependent Variation in the Bacteriophage-inactivating Capacity and Antigenicity of Lipopolysaccharide Free

Abstract

Growth temperature affected the structure of Ye 3827 lipopolysaccharide (LPS). Although Ye 3827 synthesized smooth LPS when grown at a low temperature (25 °C), partial smooth-rough transition occurred when the bacteria were grown at the physiological temperature (37 °C). The structural alteration was detected by bacteriophage-inactivation assay and chemical and immunological analyses. LPS prepared from bacteria grown at 25 °C inactivated a number of bacteriophages that recognize the O-antigenic polysaccharide portion of LPS, whereas more than 3000 times the amount of LPS from bacteria grown at 37 °C was required for the same degree of inactivation. The antigenic determinant(s) responsible for the major reaction between 25 °C-LPS and anti-25 °C-bacteria was located on the O-antigenic polysaccharide portion of LPS, but those responsible for the major reaction between 37 °C-LPS and anti-37 °C-bacteria were located on the R-core or inner portion of LPS.

  • Received:
  • Revised:
  • Published Online:
© Society for General Microbiology 1983
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-129-9-2739
1983-09-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/129/9/mic-129-9-2739.html?itemId=/content/journal/micro/10.1099/00221287-129-9-2739&mimeType=html&fmt=ahah

References

  1. Acker G., Wartenberg K., Knapp W. 1980; Zuckerzusammensetzung des Lipopolysaccharids und Feinstruktur der äusseren Membran (Zellwand) bei Yersinia enterocolitica. Zentralblatt für Bakterio-logie, Parasitenkunde, Infektionskrankheiten und Hygiene (Abteilung I,Originate A) 247:229–240
     [Google Scholar]
  2. Acker G., Knapp W., Wartenberg K., Mayer H. 1981; Localization of enterobacterial common antigen in Yersinia enterocolitica by the immunoferri-tin technique. Journal of Bacteriology 147:602–611
     [Google Scholar]
  3. Ames B.N., Dobin D.T. 1960; The role of polyamines in the neutralization of bacteriophage deoxyribonucleic acid. Journal of Biological Chemistry 235:769–775
     [Google Scholar]
  4. Darveau R.P., Charnetzky W.T., Hurlbert R.E. 1980; Outer membrane protein composition of Yersinia pestis at different growth stages and incubation temperatures. Journal of Bacteriology 143:942–949
     [Google Scholar]
  5. Davies D.A.L., Morgan W.T.J., Record B.R. 1955; Studies in immunochemistry. 15. The specific polysaccharide of the dominant ‘O’ somatic antigen of Shigella dysenteriae. Biochemical Journal 60:290–303
     [Google Scholar]
  6. Dubois C.S., Gilles K.A., Hamilton J.K., Rebers P.A., Smith F. 1956; Colorimetric method for determination of sugars and related substances. Analytical Chemistry 38:350–356
     [Google Scholar]
  7. Ellwood D.C., Kirk G.R.A. 1971; The occurrence of 6-deoxy-l-altrose in the lipopol-saccharide of Yersinia enterocolitica. Biochemical Journal 122:14
     [Google Scholar]
  8. Fromme I., Schlecht S. 1973; Influence of aeration conditions in fermentation cultures on the chemical composition of Salmonella lipopolysac-charides. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene (Abteilung I, Originate A) 224:331–344
     [Google Scholar]
  9. Galanos C., Luderritz O., Westphal O. 1969; A new method for the extraction of R lipo-polysaccharides. European Journal of Biochemistry 9:245–249
     [Google Scholar]
  10. Goldfarb A.R. 1966; A kinetic study of reactions of amino acids and peptides with the TNBS. Biochemistry 5:2570–2577
     [Google Scholar]
  11. Goldman R.C., Leive L. 1980; Heterogeneity of antigenic side chain length in lipopolysaccharide from Escherichia coli Ol 11 and Salmonella typhimur-ium LT2. European Journal of Biochemistry 107:145–153
     [Google Scholar]
  12. Hoffman J., Lindberg B., Brubaker R.R. 1980; Structural studies of the O-specific side-chains of the lipopolysaccharide from Yersinia enterocolitica Ye 128. Carbohydrate Research 78:212–214
     [Google Scholar]
  13. Jann B., Reske K., Jann K. 1975; Heterogeneity of lipopolysaccharides. Analysis of polysaccharide chain lengths by sodium dodecylsulfate-polyacryl-amide gel electrophoresis. European Journal of Biochemistry 60:239–246
     [Google Scholar]
  14. Kawaoka Y., Naiki M., Yanagawa R. 1979; Radioimmunoassay system using a serovar-specific lipopolysaccharide antigen of Leptospira. . Journal of Clinical Microbiology 10:313–316
     [Google Scholar]
  15. Kawaoka Y., Naiki M., Yanagawa R. 1982a; Isolation of the antigen-active components from leptospiral serovar-specific lipopolysaccharide antigen by mild acid hydrolysis. Japanese Journal of Veterinary Science 44:473–478
     [Google Scholar]
  16. Kawaoka Y., Otsuki K., Tsubokura M. 1982b; Characteristics of Yersinia enterocolitica bacteriophages. Zentralblatt für Bakteriologie, Parasiten-kunde, Infektionskrankheiten und Hygiene (Abteilung I, Originate A) 253:102–109
     [Google Scholar]
  17. Kawaoka Y., Otsuki K., Tsubokura M. 1982C; Temperature-dependent variation in the synthesis of the receptor for Yersinia enterocolitica bacteriophage XI. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene (Abteilung I, Originate A) 253:364–369
     [Google Scholar]
  18. Lindberg A.A., Hellerqvist C. 1980; Rough mutants of Salmonella typhimurium: immuno-chemical and structural analysis of lipopoly-saccharides from rfaH mutants. Journal of General Microbiology 116:25–32
     [Google Scholar]
  19. Maruyama T. 1973; Studies on biological characteristics and pathogenicity of Yersinia enterocolitica. I. Comparison of growth temperature range between Y. enterocolitica and other enteropathogenic bacteria. Japanese Journal of Bacteriology in Japanese 28:343–348
     [Google Scholar]
  20. Mcconnell N., Wright A. 1979; Variation in the structure and bacteriophage-inactivating capacity of Salmonella anatum lipopolysaccharide as a function of growth temperature. Journal of Bacteriology 137:746–751
     [Google Scholar]
  21. Mcintire F.C., Barlow G.G., Sievert H.W., Finley R.A., Yoo A.L. 1969; Studies on A lipopolysaccharide from Escherichia coli. Heterogeneity and mechanism of reversible inactivation by sodium deoxycholate. Biochemistry 8:4063–4067
     [Google Scholar]
  22. Mollaret H.H., Nicolle P. 1965; Sur la frequence de la lysogenie dans l’espece nouvelle Yersinia enterocolitica. . Comptes rendus hebdomadaires des seances de l’Academie des sciences 260:1027–1029
     [Google Scholar]
  23. Nikaido H. 1970; Structure of cell wall lipopolysaccharide from Salmonella typhimurium. Further studies on the linkage between O-side chains and R-core. European Journal of Biochemistry 15:57–62
     [Google Scholar]
  24. Nowotny A. 1966; Heterogeneity of endotoxic bacterial lipopolysaccharides revealed by ion-exchange column chromatography. Nature; London: 210278–280
     [Google Scholar]
  25. Okamoto K., Inoue T., Ichikawa H., Kamamoto Y., Hara S., Miyama A. 1980; Adherence of Yersinia enterocolitica to mammalian epithelial cell lines. Microbiology and Immunology 24:1013–1022
     [Google Scholar]
  26. Pai C.H., Mors V. 1978; Production of enterotoxin by Yersinia enterocolitica. Infection and Immunity 19:908–911
     [Google Scholar]
  27. Portnoy D.A., Moseley S.L., Falkow S. 1981; Characterization of plasmid and plasmid associated determinants of Yersinia enterocolitica pathogenesis. Infection and Immunity 31:775–782
     [Google Scholar]
  28. Schleifstein J., Coleman M.B. 1939; An identified microorganism resembling B. ligieresi and Pasteurella pseudotuberculosis, pathogenic for man. New York State Journal of Medicine 39:1749–1753
     [Google Scholar]
  29. Straley S.C., Brubaker R.R. 1981; Cytoplasmic and membrane proteins of yersiniae cultivated under conditions simulating mammalian intracellular environment. Proceedings of the National Academy of Sciences of the United States of America 78:1224–1228
     [Google Scholar]
  30. Straley S.C., Brubaker R.R. 1982; Localization in Yersinia pestis of peptides associated with virulence. Infection and Immunity 36:129–135
     [Google Scholar]
  31. Tsubokura M., Otsuki K., Shimohira I., Yamamoto H. 1979; Production of indirect hemolysin by Yersinia enterocolitica and its properties. Infection and Immunity 25:939–942
     [Google Scholar]
  32. Tsuji M., Kawaoka Y., Naiki M., Yanagawa R. 1981; Isolation of antigenically active components from leptospiral serovar-specific lipopolysaccharide antigen by alkaline treatment. Microbiology and Immunology 25:949–957
     [Google Scholar]
  33. Weissbach A., Hurwitz J. 1959; The formation of 2-keto-3-deoxyheptonic acid in extracts of Escherichia coli B. I. Identification. Journal of Biological Chemistry 234:705–709
     [Google Scholar]
  34. Westphal O., Jann K. 1965; Bacterial lipopolysaccharide extraction with phenol-water and further application of the procedure. Methods in Carbohydrate Chemistry 5:83–91
     [Google Scholar]
  35. Zahorchak R.J., Charnetzky W.T., Little R.V., Brubaker R.R. 1979; Consequences of Ca2+deficiency on macromolecular synthesis and adenylate energy charge in Yersinia pestis. . Journal of Bacteriology 139:792–799
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-129-9-2739
Loading
Growth Temperature-dependent Variation in the Bacteriophage-inactivating Capacity and Antigenicity of Yersinia enterocolitica Lipopolysaccharide
Microbiology 129, 2739 (1983); https://doi.org/10.1099/00221287-129-9-2739
/content/journal/micro/10.1099/00221287-129-9-2739
/content/journal/micro/10.1099/00221287-129-9-2739
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error