1887

Abstract

Spores produced by a mutant of were slow to develop their resistance properties during sporulation, and were slower to germinate than were wild-type spores. The coat protein composition of the mutant spores, as analysed by SDS-PAGE, was similar to that of the wild-type spores. However, one of the proteins (mol. wt 12000) which is normally present in the outermost layers of mature wild-type spores and which is surface-exposed, was assembled abnormally into the coat of the mutant spores and not surface-exposed. The mutation responsible for this phenotype () has been mapped between and on the chromosome, and was 47% cotransformable with This mutation, and three others closely linked to it, define a new sporulation locus, which is involved in spore coat assembly. The phenotype of the mutant(s) supports the contention that spore germination and resistance properties may be determined by the assembly of the coat.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-129-6-1945
1983-06-01
2021-07-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/129/6/mic-129-6-1945.html?itemId=/content/journal/micro/10.1099/00221287-129-6-1945&mimeType=html&fmt=ahah

References

  1. Akrigg A. 1978; Purification and properties of a manganese-stimulated deoxyribonuclease produced during sporulation of Bacillus subtilis. Biochemical Journal 172:69–76
    [Google Scholar]
  2. Anagnostopoulos C., Spizizen J. 1961; Requirements for transformation in Bacillus subtilis. Journal of Bacteriology 81:741–746
    [Google Scholar]
  3. Aronson A. I., Fitz-James P. 1976; Structure and morphogenesis of the bacterial spore coat. Bacteriological Reviews 40:360–402
    [Google Scholar]
  4. Coote J. G. 1972; Sporulation in Bacillus subtilis.Genetic analysis of oligosporogenous mutants. Journal of General Microbiology 71:17–27
    [Google Scholar]
  5. Dancer B. N., Mandelstam J. 1975; Production and possible function of serine protease during sporulation of Bacillus subtilis. Journal of Bacteriology 121:406–410
    [Google Scholar]
  6. Dion P., Mandelstam J. 1980; Germination properties as marker events characterizing later stages of Bacillus subtilis spore formation. Journal of Bacteriology 141:786–792
    [Google Scholar]
  7. Glenn A. R., Mandelstam J. 1971; Sporulation in Bacillus subtilis 168.Comparison of alkaline phosphatase from sporulating and vegetative cells. Biochemical Journal 123:129–138
    [Google Scholar]
  8. Goldman R. C., Tipper D. J. 1978; Bacillus subtilis spore coats: complexity and purification of a unique polypeptide component. Journal of Bacteriology 135:1091–1106
    [Google Scholar]
  9. Gould G. W., Stubbs J. M., King W. L. 1970; Structure and composition of resistant layers in bacterial spore coats. Journal of General Microbiology 60:347–355
    [Google Scholar]
  10. Janssen F. W., Lund A. J., Anderson L. E. 1958; Colorimetric assay for dipicolinic acid in bacterial spores. Science 127:26–27
    [Google Scholar]
  11. Jenkinson H. F. 1981; Germination and resistance defects in spores of a Bacillus subtilis mutant lacking a coat polypeptide. Journal of General Microbiology 127:81–91
    [Google Scholar]
  12. Jenkinson H. F., Kay D., Mandelstam J. 1980; Temporal dissociation of late events in Bacillus subtilis sporulation from expression of genes that determine them. Journal of Bacteriology 141:793–805
    [Google Scholar]
  13. Jenkinson H. F., Sawyer W. D., Mandelstam J. 1981; Synthesis and order of assembly of spore coat proteins in Bacillus subtilis. Journal of General Microbiology 123:1–16
    [Google Scholar]
  14. Karamata D., Gross J. D. 1970; Isolation and genetic analysis of temperature-sensitive mutants of B. subtilis defective in DNA synthesis. Molecular and General Genetics 108:277–287
    [Google Scholar]
  15. Lacks S., Hotchkiss R. D. 1960; A study of the genetic material determining an enzyme activity in Pneumococcus. Biochimica et biophysica acta 39:508–518
    [Google Scholar]
  16. Laemmli U. K., Favre M. 1973; Maturation of the head of bacteriophage T4. I. DNA packaging events. Journal of Molecular Biology 80:575–599
    [Google Scholar]
  17. Lafferty E., Moir A. 1977; Further studies on conditional germination mutants of Bacillus subtilis168. In Spore Research 1976 pp. 87–105 Barker A. N., Wolf J., Ellar D. J., Dring G. J., Gould G. W. Edited by London:: Academic Press.;
    [Google Scholar]
  18. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. Journal of Molecular Biology 3:208–218
    [Google Scholar]
  19. Moir A. 1981; Germination properties of a spore coat-defective mutant of Bacillus subtilis. Journal of Bacteriology 146:1106–1116
    [Google Scholar]
  20. Moir A., Lafferty E., Smith D. A. 1979; Genetic analysis of spore germination mutants of Bacillus subtilis 168: the correlation of phenotype with map location. Journal of General Microbiology 111:165–180
    [Google Scholar]
  21. Ohne M., Rutberg B., Hoch J. A. 1973; Genetic and biochemical characterization of mutants of Bacillus subtilis defective in succinate dehydrogenase. Journal of Bacteriology 115:738–745
    [Google Scholar]
  22. Piggot P. J. 1973; Mapping of asporogenous mutations of Bacillus subtilis: a minimum estimate of the number of sporulation operons. Journal of Bacteriology 114:1241–1253
    [Google Scholar]
  23. Sadoff H. L. 1966; Glucose dehydrogenase-soluble.I. Bacillus cereus.Methods in Enzymology 9:103–107
    [Google Scholar]
  24. Schaeffer P., Ionesco H., Ryter A., Balassa G. 1965; La sporulation de Bacillus subtilis: étude génétique et physiologique. Colloques internationaux du Centre national de la recherche scientifique 124:553–563
    [Google Scholar]
  25. Stelma G. N. Jr Aronson A. I., Fitz-James P. C. 1978; Properties of Bacillus cereus temperature-sensitive mutants altered in spore coat formation. Journal of Bacteriology 134:1157–1170
    [Google Scholar]
  26. Stelma G.N. Jr Aronson A. I., Fitz-James P. 1980; A Bacillus cereus mutant defective in spore coat deposition. Journal of General Microbiology 116:173–185
    [Google Scholar]
  27. Sterlini J. M., Mandelstam J. 1969; Commitment to sporulation in Bacillus subtilis and its relationship to development of actinomycin resistance. Biochemical Journal 113:29–37
    [Google Scholar]
  28. Stewart G.S.A.B., Ellar D. J. 1982; Characterization, purification and synthesis of spore-coat protein in Bacillus megaterium KM. Biochemical Journal 202:231–241
    [Google Scholar]
  29. Ward J. B. Jr Zahler S. A. 1973; Genetic studies of leucine biosynthesis in Bacillus subtilis. Journal of Bacteriology 116:719–726
    [Google Scholar]
  30. Young F. E., Spizizen J. 1961; Physiological and genetic factors affecting transformation of Bacillus subtilis. Journal of Bacteriology 81:823–829
    [Google Scholar]
  31. Yudkin M. D., Turley L. 1980; Suppression of asporogeny in Bacillus subtilis: allele-specific suppression of a mutation in the spoII A locus. Journal of General Microbiology 121:69–78
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-129-6-1945
Loading
/content/journal/micro/10.1099/00221287-129-6-1945
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error