SUMMARY: The growth in continuous culture of on the nitrogen sources glutamine, ammonia, or ammonia and 5-oxoproline (formed when aqueous glutamine solutions are autoclaved) is described. When growth was nitrogen-limited with autoclaved glutamine the specific rate of 5-oxoproline utilization was independent of dilution rate, whereas the specific rate of ammonia utilization increased with dilution rate. Although the culture was nitrogen-limited, excess nitrogen (as 5-oxoproline) was still present in the supernatant, especially at high dilution rates.

Values obtained for and were 72.2 and 32.5 g dry wt bacteria (mol substrate), respectively, under ammonia/5-oxoproline limitation and 95.7 and 30.2 g dry wt bacteria (mol substrate), respectively, under glutamine limitation.


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