1887

Abstract

The growth in continuous culture of on the nitrogen sources glutamine, ammonia, or ammonia and 5-oxoproline (formed when aqueous glutamine solutions are autoclaved) is described. When growth was nitrogen-limited with autoclaved glutamine the specific rate of 5-oxoproline utilization was independent of dilution rate, whereas the specific rate of ammonia utilization increased with dilution rate. Although the culture was nitrogen-limited, excess nitrogen (as 5-oxoproline) was still present in the supernatant, especially at high dilution rates.

Values obtained for and were 72·2 and 32·5 g dry wt bacteria (mol substrate), respectively, under ammonia/5-oxoproline limitation and 95·7 and 30·2 g dry wt bacteria (mol substrate), respectively, under glutamine limitation.

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/content/journal/micro/10.1099/00221287-129-6-1707
1983-06-01
2024-04-26
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References

  1. Archibald R. M. 1945; Chemical characteristics and physiological roles of glutamine. Chemical Reviews 37:161–208
    [Google Scholar]
  2. Bergersen F. J., Turner G. L., Gibson A. H., Dudman W. F. 1976; Nitrogenase activity and respiration of cultures of Rhizobium spp. with special reference to the concentration of dissolved oxygen. Biochimica et biophysica acta 444:164–174
    [Google Scholar]
  3. Jones C. W. 1978; Microbial oxidative phosphorylation. Biochemical Society Transactions 6:361–363
    [Google Scholar]
  4. Jones C. W., Brice J. M., Edwards C. 1977; The effect of respiratory chain composition on the growth efficiencies of aerobic bacteria. Archives of Microbiology 115:85–93
    [Google Scholar]
  5. Kawai J., Kawai Y., Uemura T. 1965; Studies on metabolism of pyrollidone carboxylic acid in bacteria. I. Accumulation of l-glutamic acid by bacteria in the presence of pyrollidone carboxylic acid. Agricultural and Biological Chemistry 29:395–402
    [Google Scholar]
  6. Kawai Y., Uemura T. 1966; Studies on metabolism of pyrollidone carboxylic acid in bacteria. II. l-Glutamic acid formation from pyrollidone carboxylic acid by Pseudomonas alcaligenes. . Agricultural and Biological Chemistry 33:212–219
    [Google Scholar]
  7. Kawai Y., Aida K., Uemura T. 1969; Studies on metabolism of pyrollidone carboxylic acid in bacteria. III. Cleavage of pyrollidone carboxylic acid to l-glutamic acid by the enzyme preparation obtained from Pseudomonas alcaligenes ATCC-12815, with special reference to the optical isomerisation of pyrollidone carboxylic acid by the enzyme preparation. Agricultural and Biological Chemistry 33:212–219
    [Google Scholar]
  8. Maruyama Y., Nomura M. 1956; Metabolism of pyrollidone carboxylic acid by bacteria. Journal of Biochemistry 43:327–335
    [Google Scholar]
  9. Ratcliffe H. D., Drozd J. W., Bull A. T. 1978; The utilisation of l-glutamine and the product of its thermal decomposition by Klebsiella pneumoniae and Rhizobium leguminosarum. . FEMS Microbiology Letters 3:65–69
    [Google Scholar]
  10. Ratcliffe H. D., Drozd J. W., Bull A. T. 1983; Growth energetics of Rhizobium leguminosarum grown in chemostat culture. Journal of General Microbiology 129:1697–1706
    [Google Scholar]
  11. Tosa T., Chibata I. 1965; Utilisation of cyclic amides and formation of ψ-amino acids by microorganisms. Journal of Bacteriology 89:919–920
    [Google Scholar]
  12. Van Der Werf P., Meister A. 1974; Isolation of 5-oxoprolinase from a prokaryote. Biochemical and Biophysical Research Communications 56:90–96
    [Google Scholar]
  13. Van Der Werf P., Meister A. 1975; The metabolic formation and utilisation of 5-oxo-l-proline (l-pyroglutamate, l-pyrrolidone carboxylate). Advances in Enzymology 43:519–556
    [Google Scholar]
  14. Wilcockson J., Werner D. 1978; Nitrogenase activity of Rhizobium japonicum growing on agar surfaces in relation to slime production, growth and survival. Journal of General Microbiology 108:151–160
    [Google Scholar]
  15. Wilcockson J., Werner D. 1979; Organic acids and prolonged nitrogenase activity by non-growing, free-living Rhizobium japonicum. . Archives of Microbiology 122:153–159
    [Google Scholar]
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