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A new class of temperature-sensitive mutants defective in spore germination (termed spg) was isolated and characterized. The mutants germinate normally at 19 °C but are defective at 27 °C. Analysis of the germination of these mutants at the non-permissive temperature after periods of incubation at 19 °C shows that the temperature-sensitive step in germination occurs somewhere between 60 and 120 min at 19 °C. Activated mutant spores can be deactivated by incubation at 27 °C prior to shift to the permissive temperature. The mutants are not temperature-sensitive for growth or development.
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