1887

Abstract

Summary: Chemostat-grown cells of a newly isolated strain of were flocculated by the addition of small amounts of positively charged flocculants. The flocculation ability of the cells was almost completely lost after extracting the cells with 1-5 mM-EDTA, or by reducing pH to below 4.5. Reconstitution of the flocculation ability of EDTA-extracted cells was obtained either by the addition of an excess amount of calcium ions, or by mixing dialysed EDTA extracts with EDTA-extracted cells. The EDTA extract contained mainly proteins. The major protein was purified to homogeneity by gel filtration, and had a molecular size of 65000 Dal and an isoelectric point of 9.0. Immunological techniques indicated that this protein was located on the surface of the bacteria. We, therefore, propose that the surface protein of the sp. is involved in flocculation by positively charged flocculants.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-129-12-3611
1983-12-01
2019-12-14
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-129-12-3611
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error