1887

Abstract

Chemostat-grown cells of a newly isolated strain of were flocculated by the addition of small amounts of positively charged flocculants. The flocculation ability of the cells was almost completely lost after extracting the cells with 1–5 m-EDTA, or by reducing pH to below 4·5. Reconstitution of the flocculation ability of EDTA-extracted cells was obtained either by the addition of an excess amount of calcium ions, or by mixing dialysed EDTA extracts with EDTA-extracted cells. The EDTA extract contained mainly proteins. The major protein was purified to homogeneity by gel filtration, and had a molecular size of 65000 Dal and an isoelectric point of 9·0. Immunological techniques indicated that this protein was located on the surface of the bacteria. We, therefore, propose that the surface protein of the sp. is involved in flocculation by positively charged flocculants.

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/content/journal/micro/10.1099/00221287-129-12-3611
1983-12-01
2024-12-08
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References

  1. Ashwell G. 1957; Colorimetric analysis of sugars. . Methods in Enzymology 3:73–105
    [Google Scholar]
  2. Atkinson B., Daoud I. S. 1976; Microbial floes of protein utilizing the principle of protein-dye and flocculation in fermentation process engineer- binding. Analytical Biochemistry 72:248–254
    [Google Scholar]
  3. Bradford M. M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindin. Advances in Biochemical Engineering 4:41–124
    [Google Scholar]
  4. Buckmire F.L.A., Murray R. G. E. 1970; Studies on the cell wall of Spirillum serpens. 1. Isolation and partial purification of the outermost cell wall layer. Canadian Journal of Microbiology 16:1011–1022
    [Google Scholar]
  5. Endo T., Takahashi H. 1980; Reconstitution of pronase susceptible floes of Flavobacterium sp. Agricultural and Biological Chemistry 44:2413–2418
    [Google Scholar]
  6. Endo T., Nakamura K., Takahashi H. 1976; Pronase-susceptible floe forming bacteria: relationship between flocculation and calcium ions. Agricultural and Biological Chemistry 40:2289–2295
    [Google Scholar]
  7. Häggström L. 1975 Methanol utilizing bacteria for single cell protein production. Ph.D. thesis University of Lund, Sweden.:
    [Google Scholar]
  8. Harden V. P., Harris J. O. 1953; The isoelectric point of bacterial cells. Journal of Bacteriology 65:198–202
    [Google Scholar]
  9. Harris R. H., Mitchell R. 1973; The role of polymers in microbial aggregation. Annual Review of Microbiology 27:27–50
    [Google Scholar]
  10. Howard L., Tipper D. J. 1973; A polypeptide bacteriophage receptor: modified cell wall protein subunits in bacteriophage-resistant mutants of Bacillus sphaericus strain P-1. Journal of Bacteriology 113:1491–1504
    [Google Scholar]
  11. Kabir S. 1980; Composition and immunochemical properties of outer membrane proteins of Vibrio cholerae. Journal of Bacteriology 144:382–389
    [Google Scholar]
  12. Leive L., Morrison D. C. 1972; Isolation of lipo-polysaccharides from bacteria. Methods in Enzymo-logy 28:254–262
    [Google Scholar]
  13. Masuda K., Kawata T. 1981; Characterization of a regular array in the wall of Lactobacillus buchneri and its reattachment to the other wall components. Journal of General Microbiology 124:81–90
    [Google Scholar]
  14. Mogren H. 1979; SCP from methanol - the Norprotein process. Process Biochemistry 143:2–7
    [Google Scholar]
  15. Osborn M. J., Wu H.C.P. 1980; Proteins of the outer membrane of Gram-negative bacteria. Annual Review of Microbiology 34:369–422
    [Google Scholar]
  16. Pipes W. O. 1978; Microbiology of activated sludge bulking. Advances in Applied Microbiology 24:85–127
    [Google Scholar]
  17. Scott T. A., Melvin E. H. 1953; Determination of dextran with anthrone. Analytical Chemistry 25:1656–1661
    [Google Scholar]
  18. Senior P. J., Windass J. 1980; The ICI single cell protein process. Biotechnology tetters 2:205–210
    [Google Scholar]
  19. Sleytr U. B. 1978; Regular arrays of macromolecules on bacterial cell walls: structure, chemistry, assembly and function. International Review of Cytology 53:1–64
    [Google Scholar]
  20. Sleytr U. B., Thorne K.J.I. 1976; Chemical characterization of the regularly arranged surface layers of Clostridium thermosaccharolyticum and Clostridium thermohydrosulfuricum. Journal of Bacteriology 126:377–383
    [Google Scholar]
  21. Thorne K.J.I. 1977; Regularly arranged protein on the surfaces of Gram-negative bacteria. Biological Reviews 52:219–234
    [Google Scholar]
  22. Thornley M. J., Glauert A. M., Sleytr U. B. 1974; Structure and assembly of bacterial surface layers composed of regular arrays of subunits. Philosophical Transactions of the Royal Society. Series B 268:147–153
    [Google Scholar]
  23. Wang D.I.C., Sinskey A. J. 1970; Collection of microbial cells. Advances in Applied Microbiology 12:121–152
    [Google Scholar]
  24. Weber K., Osborn M. 1969; The reliability of molecular weight determinations by dodecyl sulfate- polyacrylamide gel electrophoresis. Journal of Biological Chemistry 244:4406–4412
    [Google Scholar]
  25. Wilkinson J. F. 1971; Hydrocarbons as a source of single-cell protein. Symposia of the Society for General Microbiology 21:15–46
    [Google Scholar]
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