1887

Abstract

Sixty-eight of 519 strains of and six of 10 strains of produced toxins acting on Vero cells (VT); all of 63 and strains were VT. Most of the VT strains were from weaned pigs suffering from oedema disease and/or diarrhoea and belonged to serogroups O141:K85,88, O141:K85, O138:K81, and O139:K82; six VT . strains were from diarrhoeic human babies, four of serogroup O26 and two of serogroup O128. The VT genes in two of the O26 strains and in the O128 strains were located in the genome of the phages with which they were lysogenized. One O141:K85,88 pig strain transferred its VT genes, probably by conjugation, to K12. The VTs of the human strains, the pig strains and the strains were antigenically different from each other; unlike the others, the VT was heat-resistant. Cell-free preparations of cultures of K12 to which the VT genes of the four human strains had been transferred caused fluid accumulation in ligated segments of rabbit intestine. Inoculated intravenously, they were lethal for mice and rabbits; similar preparations of K12 to which the VT genes of the pig strain had been transferred produced a disease in pigs that clinically and pathologically resembled oedema disease.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-129-10-3121
1983-10-01
2022-01-22
Loading full text...

Full text loading...

/deliver/fulltext/micro/129/10/mic-129-10-3121.html?itemId=/content/journal/micro/10.1099/00221287-129-10-3121&mimeType=html&fmt=ahah

References

  1. Clugston R.E., Nielsen N.O. 1974; Experimental oedema disease of swine (E. coli enterotoxemia). I. Detection and preparation of an active principle. Canadian Journal of Comparative Medicine 38:22–28
    [Google Scholar]
  2. Evans D.G., Evans D.J., Pierce N.F. 1973; Differences in the response of rabbit small intestine to heat-labile and heat-stable enterotoxins of Escherichia coli . Infection and Immunity 7:873–880
    [Google Scholar]
  3. Glover S.W. 1968 In Experiments in Microbial Genetics p. 17 Edited by Clowes R. C., Hayes W. Oxford & Edinburgh: Blackwell Scientific Publications;
    [Google Scholar]
  4. Hirota Y. 1960; The effect of acridine dyes on mating type factors in Escherichia coli . Proceedings of the National Academy of Sciences of the United States of America 46:57–64
    [Google Scholar]
  5. Kashiwazaki M., Ogawa T., Isayama Y., Akaike Y., Tamura K., Sakazaki R. 1980; Detection of Vero cytotoxic strains of Escherichia coli isolated from diseased animals. National Institute of Animal Health Quarterly (Japan) 20:116–117
    [Google Scholar]
  6. Kashiwazaki M., Ogawa T., Nakamura K., Isayama T., Tamura K., Sakazaki R. 1981; Vero cytotoxin produced by Escherichia coli strains of animal origin. National Institute of Animal Health Quarterly (Japan) 21:69–72
    [Google Scholar]
  7. Konowalchuk J., Speirs J.I., Stavric S. 1977; Vero response to a cytotoxin of Escherichia coli . Infection and Immunity 18:775–779
    [Google Scholar]
  8. O’Brien A.D., Laveck G.D., Thompson M.R., Formal S.B. 1982; Production of Shigella dysenteriae Type 1-like cytotoxin by Escherichia coli . Journal of Infectious Diseases 146:763–769
    [Google Scholar]
  9. O’Brien J.I., Lively T.A., Chen M.E., Rothman S.W., Formal S.B. 1983; Escherichia coli 0157 :H7. Strains associated with haemorrhagic colitis in the United States produce a Shigella dysenteriae 1 (Shiga)-like cytotoxin. Lancet i:702
    [Google Scholar]
  10. Scotland S.M., Day N.P., Rowe B. 1979; Production by strains of Escherichia coli of a cytotoxin (VT) affecting Vero cells. Society for General Microbiology Quarterly 6:156–157
    [Google Scholar]
  11. Scotland S.M., Day N. P., Rowe B. 1980a; Production of a cytotoxin affecting Vero cells by strains of Escherichia coli belonging to traditional enteropathogenic serogroups. FEMS Microbiology Letters 7:15–17
    [Google Scholar]
  12. Scotland S.M., Day N. P., Willshaw G. A., Rowe B. 1980b; Cytotoxic enteropathogenic Escherichia coli . Lancet i:90
    [Google Scholar]
  13. Smith H., Williams, Gyles C.L. 1970; The relationship between two apparently different enterotoxins produced by enteropathogenic strains of Escherichia coli of porcine origin. Journal of Medical Microbiology 3:387–401
    [Google Scholar]
  14. Smith H., Williams, Linggood M. A. 1971; The transmissible nature of enterotoxin production in a human enteropathogenic strain of Escherichia coli . Journal of Medical Microbiology 4:301–305
    [Google Scholar]
  15. Tomoeda M., Inuzuka M., Kubo N., Nakama S. 1968; Effective elimination of drug resistance and sex factors in Escherichia coli by sodium dodecylsulphate. Journal of Bacteriology 95:1078–1089
    [Google Scholar]
  16. Wade W.G., Thom B.T., Evans N. 1979; Cytotoxic enteropathogenic Escherichia coli . Lancet ii:1235–1236
    [Google Scholar]
  17. Williams P.H., Sedgewick M.I., Evans N., Turner P.J., George R.H., Mcneish A.S. 1978; Adherence of enteropathogenic strains of Escherichia coli to human intestinal mucosa is mediated by a colicinogenic conjugative plasmid. In fection and Immunity 22:393–402
    [Google Scholar]
  18. Wilson M.W., Bettelheim K.A. 1980; Cytotoxic Escherichia coli serotypes. Lancet i:201
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-129-10-3121
Loading
/content/journal/micro/10.1099/00221287-129-10-3121
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error