1887

Abstract

Crude cell extracts of the fission yeast (strains NCYC 132 and 972h) can phosphorylate dihydroxyacetone but not glycerol; activity for -glyceraldehyde is very low. This suggests that a specific dihydroxyacetone kinase is present and catalyses the second step in a pathway for glycerol utilization, in which the initial step is an oxidation by an NAD-linked glycerol dehydrogenase. In support of this pathway (glycerol→dihydroxyacetone→dihydroxyacetone phosphate), both strains can utilize glycerol or dihydroxyacetone. but not -glyceraldehyde, as growth substrates. Both enzymes are subject to catabolite repression and may also be inducible but are not co-ordinately regulated.

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/content/journal/micro/10.1099/00221287-128-8-1763
1982-08-01
2021-07-26
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