Summary: Comparative immunodiffusion studies and thin-layer chromatographic analyses of whole-organism acid methanolysates were performed on 37 strains of and . The latter were clearly distinguished from the mycobacteria in containing a single mycolic acid methyl ester and showing more precipitinogens with nocardial than with mycobacterial and rhodococcal reference systems. The distribution of precipitinogens showed that and were very closely related and that both showed a greater affinity to than to any of the other established species of tested. The complex pattern of α-mycolates and characteristic polar mycolates found in both and has only previously been found in and .


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