1887

Abstract

The ability of four genetically distinct plasmid-located tetracycline resistance determinants (Tet A, B, C and D) to confer decreased accumulation of tetracycline and some of its analogues by K12 was examined. Accumulation of oxytetracycline, tetracycline, demethylchlorotetracycline, 6-demethyl-6-deoxy-5-hydroxy-6-methylene-tetracycline, chlorotetracycline, doxycycline and 6-demethyl-6-deoxytetracycline was examined by fluorescence spectroscopy. The determinants varied in their ability to promote decreased accumulation of tetracyclines, defined as an R/R fluorescence ratio of < 0·85. Plasmid pIP7 (TetA) caused reduced accumulation of only oxytetracycline, tetracycline and chlorotetracycline, but plasmid pDU301 (TetB) promoted reduced accumulation of all the compounds tested except 6-demethyl-6-deoxytetracycline. The TetC determinant of pBR322 caused decreased uptake of five derivatives, but not doxycycline or 6-demethyl-6-deoxytetra-cycline. Plasmid RA1 (TetD) encoded reduced accumulation of oxytetracycline, tetracycline, 6-demethyl-6-deoxy-5-hydroxy-6-methylenetetracycline and chlorotetracycline. In general, the resistance determinants were more efficient in promoting decreased accumulation of hydrophilic tetracyclines. These accumulation studies provide a satisfactory method for the phenotypic identification of Tet resistance determinants.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-128-4-689
1982-04-01
2021-10-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/128/4/mic-128-4-689.html?itemId=/content/journal/micro/10.1099/00221287-128-4-689&mimeType=html&fmt=ahah

References

  1. Achtman M., Willetts N., Clark A. J. 1971; Beginning a genetic analysis of conjugational transfer determined by the F factor in Escherichia coli by isolation and characterization of transfer-deficient mutants. Journal of Bacteriology 106:529–538
    [Google Scholar]
  2. Ball P. R., Shales S. W., Chopra I. 1980; Plasmid-mediated tetracycline resistance in Escherichia coli involves increased efflux of the antibiotic. Biochemical and Biophysical Research Communications 93:74–81
    [Google Scholar]
  3. Boyer H. W., Betlach M., Bolivar F., Rodriguez R. L., Heyneker H. L., Shine J., Goodman H. M. 1977; The construction of molecular cloning vehicles. In Recombinant Molecules: Impact on Science and Society pp. 9–20 Edited by Beers R. F., Bassett E. G. New York: Raven Press;
    [Google Scholar]
  4. Bukhari A. I., Shapiro J. L., Adhya S. L. editors 1977 DNA Insertion Elements, Plasmids and Episomes Cold Spring Harbor, New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  5. Chopra I., Howe T. G. B., Linton A. H., Linton K. B., Richmond M. H., Speller D. C. E. 1981a; The tetracyclines: prospects at the beginning of the 1980s. Journal of Antimicrobial Chemotherapy 8:5–21
    [Google Scholar]
  6. Chopra I., Shales S. W., Ward J. M., Wallace L. J. 1981b; Reduced expression of TnlO-mediated tetracycline resistance in Escherichia coli containing more than one copy of the transposon. Journal of General Microbiology 126:45–54
    [Google Scholar]
  7. Chopra I., Ball P. R., Eccles S. J., Shales S. W. 1981c; Nature and function of TnlO-encoded proteins that mediate tetracycline resistance in Escherichia coli. In Molecular Biology, Pathogenicity, and Ecology of Bacterial Plasmids p. 592 Edited by Levy S. B. New York: Plenum Publishing Corp;
    [Google Scholar]
  8. Colaizzi J. L., Klink P. R. 1969; pH-partition behaviour of tetracyclines. Journal of Pharmaceutical Sciences 58:1184–1189
    [Google Scholar]
  9. Levy S. B., Mcmurry L., Onigman P., Saunders R. M. 1977; Plasmid-mediated tetracycline resistance in Escherichia coli. . In Topics in Infectious Diseases 2 pp. 181–203 Edited by Drews J., Hogenauer G. New York: Springer Verlag;
    [Google Scholar]
  10. Mcmurry L., Petrucci R. E., Levy S. B. 1980; Active efflux of tetracycline encoded by four genetically different tetracycline resistance determinants in Escherichia coli. . Proceedings of the National Academy of Sciences of the United States of America 773974–3977
    [Google Scholar]
  11. Mendez B., Tachibana C., Levy S. B. 1980; Heterogeneity of tetracycline resistance determinants. Plasmid 3:99–108
    [Google Scholar]
  12. Samra Z., Krauszsteinmetz J., Sompolinsky D. 1978; Transport of tetracyclines through the bacterial cell membrane assayed by fluorescence-study with susceptible and resistant strains of Staphylococcus aureus and Escherichia coli. . Microbios 21:7–12
    [Google Scholar]
  13. Shales S. W., Chopra I., Ball P. R. 1980; Evidence for more than one mechanism of plasmid-determined tetracycline resistance in Escherichia coli. . Journal of General Microbiology 121:221–229
    [Google Scholar]
  14. Sutcliffe J. G. 1978; Complete nucleotide sequence of the Escherichia coli plasmid pBR322. Cold Spring Harbor Symposia on Quantitative Biology 43:77–90
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-128-4-689
Loading
/content/journal/micro/10.1099/00221287-128-4-689
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error