1887

Abstract

The effects of N and P limitation on cell division and on cellular contents of limiting and non-limiting nutrients were examined in cyclostat cultures of the freshwater diatoms and Nutrient-limited cell cycles of both species were readily entrained by a 14 h/10 h light/dark cycle. In , the maxima relative to the onset of the light phase of the photocycle occurred at approximately − 1 to +1 h for cell division, +11 to 13 h for cell N, and +21 to 23 h for cell P. In the maxima occurred at approximately +3 to 10 h for cell division and +2 to 4 h for cell N and cell P. Changes in culture dilution rate (integrated growth) and in the type of nutrient limiting growth did not significantly alter these times for either species. The cell-number oscillations can be explained by a simple exponential function for the instantaneous population growth rate, which occurs after a single modal transition time τ, which is characteristic for each species. The consistency of phase relations between N and P limitations and culture dilution rates suggests that the temporal organization for events within the cell cycle of each organism is independent of the nutrient-limited status of the cell populations.

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/content/journal/micro/10.1099/00221287-128-1-199
1982-01-01
2021-07-28
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References

  1. Chisholm S. W. 1981; Temporal patterns of cell division in unicellular algae. In Physiological Phytoplankton Ecology (in the Press). Platt T. Edited by Lapari, Sicily: NATO Advanced Study Institute;
    [Google Scholar]
  2. Chisholm S. W., Costello J. C. 1980; Influence of environmental factors and population composition on the timing of cell division in Thalassiosira fluviatilis (Bacillariophyceae) grown on light/dark cycles. Journal of Phycology 16:375–383
    [Google Scholar]
  3. Chisholm S. W., Stross R. G., Nobbs P. A. 1975; Light/dark phased cell division in Euglena gracilis (Z) (Euglenophyceae), in PO4 limited cultures. Journal of Phycology 11:367–373
    [Google Scholar]
  4. Chisholm S. W., Azam F., Eppley R. W. 1978; Silicic acid incorporation in marine diatoms on light/dark cycles: use as an assay for phased cell division. Limnology and Oceanography 23:518–529
    [Google Scholar]
  5. Chisholm S. W., Morel F. M. M., Slocum W. S. 1981; The phasing and distribution of cell division cycles in marine diatoms. Brookhaven Symposia in Biology 31:281–300
    [Google Scholar]
  6. Edmunds L. J.Jr Cirillo V. P. 1974; On the interplay among cell cycle, biological clock and membrane transport control systems. International Journal of Chronobiology 2:233–264
    [Google Scholar]
  7. Frisch H. K., Gotham I. J. 1977; On periodic cyclostat populations. Journal of Theoretical Biology 66:665–678
    [Google Scholar]
  8. Frisch H. L., Gotham I. J. 1979; A simple model for periodic cyclostat growth of algae. Journal of Mathematical Biology 7:149–169
    [Google Scholar]
  9. Glattli H., Dawson P. S. S. 1974; Incorporation of phosphorus into phased cells of Candida utilis using 32P and 33P. Archives of Microbiology 95:237–254
    [Google Scholar]
  10. Gotham I. J. 1977 Nutrient limited cyclostat growth: a theoretical and physiological analysis. Ph.D. thesis; State University of New York at Albany, U.S.A;
    [Google Scholar]
  11. Gotham I. J., Frisch H. L. 1981; A simple model for cell volume and developmental compartments in nutrient-limited cyclostat cultures of algae. Journal of Theoretical Biology 92:435–467
    [Google Scholar]
  12. Gotham I. J., Rhee G-Y. 1981a; Comparative kinetic studies of phosphate-limited growth and phosphate uptake in phytoplankton in continuous culture. Journal of Phycology 17:257–265
    [Google Scholar]
  13. Gotham I. J., Rhee G-Y. 1981b; Comparative kinetic studies of nitrate-limited growth and nitrate uptake in phytoplankton in continuous culture. Journal of Phycology 17:309–314
    [Google Scholar]
  14. Guillard R. R. L., Lorenzen C. J. 1972; Yellow-green algae with chlorophyllide c. Journal of Phycology 8:10–14
    [Google Scholar]
  15. Klevecz R. R. 1976; Quantized generation time in mammalian cells as an expression of the cellular clock. Proceedings of the National Academy of Sciences of the United States of America 73:4012–4016
    [Google Scholar]
  16. Rhee G-Y. 1978; Effects of N :P atomic ratios and nitrate limitation on algal growth, cell composition, and nitrate uptake. Limnology and Oceanography 23:10–25
    [Google Scholar]
  17. Rhee G-Y., Gotham I. J. 1981a; The effect of environmental factors on phytoplankton growth: temperature and the interactions of temperature with nutrient limitation. Limnology and Oceanography 26:635–648
    [Google Scholar]
  18. Rhee G-Y., Gotham I. J. 1981b; The effect of environmental factors on phytoplankton growth: light and the interactions of light with nitrate limitation. Limnology and Oceanography 26:649–659
    [Google Scholar]
  19. Rhee G-Y., Gotham I. J., Chisholm S. W. 1981; Use of cyclostat cultures to study phytoplankton ecology. In Continuous Culture of Cells 2 pp. 159–186 Calcott P. H. Edited by Ohio: CRC Press;
    [Google Scholar]
  20. Smith J. A., Martin L. 1974; Regulation of cell proliferation. In Cell Cycle Controls pp. 43–60 Padilla G. M., Cameron I. L., Zimmerman A. . Edited by New York: Academic Press;
    [Google Scholar]
  21. Stross G. G., Pemrick S. M. 1974; Nutrient uptake kinetics in phytoplankton: a basis for niche separation. Journal of Phycology 10:164–169
    [Google Scholar]
  22. Williams F. M. 1971; Dynamics of microbial populations. In Systems Analysis and Simulation in Ecology 1 pp. 197–267 Patten B. C. Edited by New York: Academic Press;
    [Google Scholar]
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