The synthesis of 1,3-β-glucanase, β--acetylglucosaminidase and total protein was studied during autolysis of Evidence for protein synthesis during autolysis was obtained from the incorporation of [H]tryptophan and [H]phenylalanine into the protein pool and from the inhibitory effects of cycloheximide and puromycin. Glucose accumulated in the growth medium during autolysis and -acetylglucosamine and two unidentified oligosaccharides were detected towards the end of the 46 d autolysis period. During autolysis, 1,3-β-glucanase accumulated in the medium to tenfold greater activities than β--acetylglucosaminidase. The former enzyme had a pH optimum of 5·5; and a of O 45 mM. It was inhibited by excess substrate but not by excess glucose.


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