1887

Abstract

The synthesis of 1,3-β-glucanase, β--acetylglucosaminidase and total protein was studied during autolysis of Evidence for protein synthesis during autolysis was obtained from the incorporation of [H]tryptophan and [H]phenylalanine into the protein pool and from the inhibitory effects of cycloheximide and puromycin. Glucose accumulated in the growth medium during autolysis and -acetylglucosamine and two unidentified oligosaccharides were detected towards the end of the 46 d autolysis period. During autolysis, 1,3-β-glucanase accumulated in the medium to tenfold greater activities than β--acetylglucosaminidase. The former enzyme had a pH optimum of 5·5; and a of O 45 mM. It was inhibited by excess substrate but not by excess glucose.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-126-2-347
1981-10-01
2019-10-21
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-126-2-347
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error