SUMMARY: Mutagenesis of yielded two major classes of mutant, both having cell surface polysaccharides fundamentally different from the wild-type. The wild-type bacterium produced copious amounts of extracellular slime polysaccharide containing glucose, mannose and glucuronic acid in a ratio of 2:2:1. ‘Non-mucoid’ mutants produced trace amounts of exopolysaccharide identical to the wild-type product; ‘crenated’ mutants produced material with an unusual composition containing sugars normally found in the lipopolysaccharide. Analysis of lipopolysaccharide fractions from these strains showed that the wild-type polysaccharide fraction contained predominantly glucose. Polysaccharides from the two classes of mutant bacteria were similar and contained rhamnose, galactose and smaller amounts of glucose.


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