1887

Abstract

Phenotypically determined resistance of gonococci to killing by normal human serum can be generated by growth of susceptible organisms in media containing guinea pig, calf or human serum. However, even in the best medium tested, i.e. defined medium containing 50% (v/v) guinea pig serum, resistance was greatly reduced after 24 h incubation and the maximum number of colony-forming units generated was 10 to 10 ml. Resistance was not acquired after incubation in guinea pig serum at low temperature (8 °C), supporting previous indications that metabolic activity was necessary for the generation of resistance. Alteration of the concentration of glutamine, proline, lactate or iron had little or no effect on the generation of serum resistance under the conditions used. Optimum conversion to resistance occurred at pH 6·0 to 6·5 and both non-diffusible and diffusate fractions of dialysed guinea pig serum promoted resistance. Furthermore, resistant organisms could be produced by incubation in defined medium containing the diffusate from guinea pig serum and 0·1% bovine serum albumin, a step which should facilitate identification of the resistance-promoting factor.

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1981-02-01
2021-07-30
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References

  1. Goldner M., Penn C.W., Sanyal S.C., Veale D.R., Smith H. 1979; Phenotypically determined resistance of Neisseria gonorrhoeae to normal human serum: environmental factors in subcutaneous chambers in guinea pigs. Journal of General Microbiology 114:169–177
    [Google Scholar]
  2. Hebeler B.H., Morse S.A. 1976; Physiology and metabolism of pathogenic Neisseria: tricarboxylic acid cycle activity in Neisseria gonorrhoeae . Journal of Bacteriology 128:192–201
    [Google Scholar]
  3. Mccutchan J.A., Levine S., Braude A.I. 1976; Influence of colony type on susceptibility of gonococci to killing by human serum. Journal of Immunology 116:1652–1655
    [Google Scholar]
  4. Magnusson K.-E., KihlstrÖm E., Norlander L., Norqvist A., Davies J., Normark S. 1979a; Effect of colony type and pH on surface charge and hydrophobicity of Neisseria gonorrhoeae . Infection and Immunity 26:397–401
    [Google Scholar]
  5. Magnusson K.-E., KihlstrÖm E., Norqvist A., Davies J., Normark S. 1979b; Effect of iron on surface charge and hydrophobicity of Neisseria gonorrhoeae . Infection and Immunity 26:402–407
    [Google Scholar]
  6. Morse S.A., Bartenstein L. 1980; Purine metabolism in Neisseria gonorrhoeae: the requirement for hypoxanthine. Canadian Journal of Microbiology 26:13–20
    [Google Scholar]
  7. Morse S.A., Hebeler B.H. 1978; Effect of pH on growth and glucose metabolism of Neisseria gonorrhoeae. . In Immunobiology of Neisseria gonorrhoeae pp. 9–17 Brooks G.F., Gotschlich E.C., Holmes K.K., Sawyer W.D., Young F.E. Edited by Washington, D.C.:: American Society for Microbiology.;
    [Google Scholar]
  8. Norlander L., Davies J., Normark S. 1979a; Genetic exchange mechanisms in Neisseria gonorrhoeae . Journal of Bacteriology 138:756–761
    [Google Scholar]
  9. Norlander L., Davies J., Norqvist A., Normark S. 1979b; Genetic basis for colonial variation in Neisseria gonorrhoeae . Journal of Bacteriology 138:762–769
    [Google Scholar]
  10. Penn C.W., Sen D., Veale D.R., Parsons N.J., Smith H., Witt K. 1976; Morphological, biological and antigenic properties of Neisseria gonorrhoeae adapted to growth in guinea-pig subcutaneous chambers. Journal of General Microbiology 97:35–43
    [Google Scholar]
  11. Penn C.W., Veale D.R., Rittenberg S.C., Smith H. 1977a; The properties of gonococci in vivo and in vitro: selection and phenotypic change in relation to virulence. In Gonorrhoea: Epidemiology and Pathogenesis pp. 117–131 Skinner F.A., Smith H., Walker P.D. Edited by London:: Academic Press.;
    [Google Scholar]
  12. Penn C.W., Veale D.R., Smith H. 1977b; Selection from gonococci grown in vitro of a colony type with some virulence properties of organisms adapted in vivo . Journal of General Microbiology 100:147–158
    [Google Scholar]
  13. Rittenberg S.C., Penn C.W., Parsons N.J., Veale D.R., Smith H. 1977; Phenotypic changes in the resistance of Neisseria gonorrhoeae to killing by normal human serum. Journal of General Microbiology 103:69–75
    [Google Scholar]
  14. Schoolnik G.K., Buchanan T.M., Holmes K.K. 1976; Gonococci causing disseminated gono-coccal infection are more resistant to the bactericidal action of normal human sera. Journal of Clinical Investigation 58:1163–1173
    [Google Scholar]
  15. Spink W.W., Keefer C.S. 1937; Studies on gonococcus infection II. The bacteriolytic power of whole defibrinated blood of patients with gonococcal arthritis. Journal of Clinical Investigation 16:117–183
    [Google Scholar]
  16. Swanson J. 1977; Surface components associated with gonococcal-cell interactions. In The Gonococcus pp. 369–402 Roberts R.B. Edited by New York:: John Wiley.;
    [Google Scholar]
  17. Veale D.R., Smith H., Witt K.A., Marshall R.B. 1975; Differential ability of colonial types of Neisseria gonorrhoeae to produce infection and an inflammatory response in subcutaneous perforated plastic chambers in guinea pigs and rabbits. Journal of Medical Microbiology 8:325–335
    [Google Scholar]
  18. Ward M.E., Watt P.J., Glynn A.A. 1970; Gonococci in urethral exudates possess a virulence factor lost on subculture. Nature; London: 227382–384
    [Google Scholar]
  19. Ward M.E., Lambden P.R., Heckels J.E., Watt P.J. 1978; The surface properties of Neisseria gonorrhoeae: determinants of susceptibility to antibody complement killing. Journal of General Microbiology 108:205–212
    [Google Scholar]
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