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The apnA1 mutation strongly reduces l-asparagine utilization in Aspergillus nidulans. The ahr A1 mutation, leading to loss of an l-asparaginase (Drainas et al., 1977), eliminates residual l-asparagine utilization in double mutant strains also carrying apnA1. This additivity suggests that A. nidulans, like Saccharomyces cerevisiae (Jones, 1977; Dunlop et al., 1978), has two l-asparaginases specified by apnA and ahrA, respectively. apnA has been mapped to a position on the left arm of linkage group II, in the sequence adH-acrA-apnA-wA-methA-palcA-(centromere).
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