1887

Abstract

SUMMARY: A fluorescent derivative of polymyxin (DANSP) was prepared by coupling 1-dimethylaminonaphthalene-5-sulphonyl chloride with the amino group of diaminobutyric acid radicals in the polymyxin molecule. As in the case of polymyxin, DANSP is readily absorbed by polymyxin-sensitive organisms and is rapidly bactericidal; cells can be protected against this bactericidal activity by pretreatment with certain cations. Mechanical disintegration of DANSP-treated cells showed that the fluorescent compound was associated with two fractions: () cell walls and () small particles. Fractionation of three strains of showed that the fluorescent conjugate was equally distributed between the two fractions, but in the case of three Gram-positive, lysozyme-sensitive, organisms 90 % of the fluorescent conjugate was found in the small particle fraction; controlled lysozyme treatment of these cells showed that the DANSP was associated with a membrane underlying the cell wall from which the small particle fraction is formed on mechanical disintegration.

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/content/journal/micro/10.1099/00221287-12-2-226
1955-04-01
2021-07-28
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References

  1. Bell P.H., Bone J.F., English J.P., Fellows C.E., Howard K.S., Rogers M.M., Shepherd R.G., Winterbottom R., Dornbush A.C., Kushner S., Subbarow Y. 1949; Chemical studies on polymyxin: comparison with ‘ Aerosporin’. Ann. N.Y. Acad. Sci. 51:897
    [Google Scholar]
  2. Bliss E.A., Chandler C.A., Schoenbach E.B. 1949; In vitro studies of polymyxin. Ann. N.Y. Acad. Sci. 51:944
    [Google Scholar]
  3. Burdon K.L. 1946; Fatty material in bacteria and fungi revealed by staining dried fixed slide preparations. J. Bact. 52:665
    [Google Scholar]
  4. Few A.V. 1954; Electron microscopy of disrupted bacteria treated with polymyxin E. J. gen. Microbiol. 10: 304
    [Google Scholar]
  5. Few A.V., Schulman J.H. 1953; The absorption of polymyxin E by bacteria and bacterial cell walls and its bactericidal action. J. gen. Microbiol. 9:454
    [Google Scholar]
  6. Latterade C., Macheboeuf M. 1950; Recherches biochimiques sur le mode d’action de la polymyxine. Ann. Inst. Pasteur 78:753
    [Google Scholar]
  7. Mickle H. 1948; Tissue disintegration. J. R. micr. Soc. 68:10
    [Google Scholar]
  8. Mitchell P., Moyle J. 1951; The glycerophospho-protein complex envelope of Micrococcus pyogenes. J. gen. Microbiol. 5:981
    [Google Scholar]
  9. Mitchell P., Moyle J. 1954; The Gram reaction and cell composition: Nucleic acids and other phosphate fractions. J. gen. Microbiol. 10:533
    [Google Scholar]
  10. Newton B.A. 1953a; The release of soluble constituents from washed cells of Pseudomonas aeruginosa by the action of polymyxin. J. gen. Microbiol. 9:54
    [Google Scholar]
  11. Newton B.A. 1953b; The reversal of the antibacterial activity of polymyxin by divalent cations. Nature; Lond.: 172,160
    [Google Scholar]
  12. Newton B.A. 1954a; Site of action of polymyxin on Pseudomonas aeruginosa: antagonism by cations. J. gen. Microbiol. 10:491
    [Google Scholar]
  13. Newton B.A. 1954b; The absorption of polymyxin by cell wall preparations from Pseudomonas aeruginosa. . J. gen. Microbiol. 10:iii
    [Google Scholar]
  14. Salton M.R.J. 1952; Studies on the bacterial cell wall. 3. Preliminary investigation of the chemical constitution of the cell wall of Streptococcus faecalis. Biochim. biophys. Acta 8:510
    [Google Scholar]
  15. Salton M.R.J. 1953; Studies on the bacterial cell wall. 4. The composition of the cell walls of some Gram positive and Gram negative bacteria. Biochim. biophys. Acta 10:512
    [Google Scholar]
  16. Stanier R.Y., Gunsalus I.C., Gunsalus C. 1953; The enzymatic conversion of mandelic acid to benzoic acid. 2. Properties of the particulate fractions. J. Boot. 66:543
    [Google Scholar]
  17. Weber G. 1952; Polarization of the fluorescence of macromolecules. 2. Fluorescent conjugates of ovalbumin and bovine serum albumin. Biochem. J. 51:155
    [Google Scholar]
  18. Weber G. 1953; Polarization of the fluorescence of labelled protein molecules. Disc. Faraday Soc. 13:33
    [Google Scholar]
  19. Weibull C. 1953; The isolation of protoplasts from Bacillus megaterium by controlled treatment with lysozyme. J. Bact. 66:688
    [Google Scholar]
  20. Wilkinson S. 1949; Crystalline derivatives of polymyxins and the identification of the fatty acid component. Nature; Lond.: 164622
    [Google Scholar]
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