1887

Abstract

Two strains of , one of which (strain 1234) was resistant to high concentrations of streptomycin, were used to inoculate plots of French beans (). Bacteria were isolated from nodules and typed using their intrinsic resistance to low concentrations of seven antibiotics. The inoculum strains were a minority of isolates from inoculated plots. The high concentration streptomycin resistance character was used in the case of strain 1234 to confirm the accuracy of identification. The resident population of was shown to be heterogeneous; 54 different resistance patterns were recorded. Isolates having the same intrinsic resistance pattern, with few exceptions, were uniform in their reaction with antiserum raised against one of the inoculum strains and in their colony morphology.

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1980-06-01
2021-05-17
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References

  1. Beringer J. E. 1974; R factor transfer in Rhizobium leguminosarum. . Journal of General Microbiology 84:188–198
    [Google Scholar]
  2. Dudman W. F., Brockwell J. 1968; Ecological studies of root nodule bacteria introduced into field environments. I. A survey of field performance of clover inoculants by gel immune diffusion serology. Australian Journal of Agricultural Research 19:739–747
    [Google Scholar]
  3. Gane A. J., King J. M., Gent G. P., Biddle A. J., Handley R. P., Bingham R. J. B. 1975 Processors and Growers Research Organisation Pea and Bean Growing Handbook 1 Beans. Processors and Growers Research Organisation:
    [Google Scholar]
  4. Graham P. H. 1963a; Antigenic affinities of the root-nodule bacteria of legumes. Antonie van Leeuwenhoek 29:281–291
    [Google Scholar]
  5. Graham P. H. 1963b; Antibiotic sensitivities of the root nodule bacteria. Australian Journal of Biological Sciences 16:557–559
    [Google Scholar]
  6. Hughes D. Q., Vincent J. M. 1942; Serological studies of the root-nodule bacteria. III. Tests of neighbouring strains of the same species. Proceedings of the Linnean Society of New South Wales 67:142–152
    [Google Scholar]
  7. Jones D. G., Bromfield E. S. P. 1978; A study of the competitive ability of streptomycin and spectinomycin mutants of Rhizobium trifolii using various marker techniques. Annals of Applied Biology 88:445–487
    [Google Scholar]
  8. Josey D. P., Beynon J. L., Johnston A. W. B., Beringer J. E. 1979; Strain identification in Rhizobium using intrinsic antibiotic resistance. Journal of Applied Bacteriology 46:343–350
    [Google Scholar]
  9. Kishinevsky B., Bar-Joseph M. 1978; Rhizobium strain identification in Arachis hypogaea nodules by enzyme-linked immunosorbent assay (ELISA). Canadian Journal of Microbiology 24:1537–1543
    [Google Scholar]
  10. Koonty F. P., Faber J. E. 1961; Somatic antigens of Rhizobium japonicum. . Soil Science 91:228
    [Google Scholar]
  11. Mahler R. L., Bezdicek D. F. 1978; Diversity of Rhizobium leguminosarum in the Palouse of Eastern Washington. Applied and Environmental Microbiology 36:780–782
    [Google Scholar]
  12. Means U. M., Johnson H. W., Date R. A. 1964; Quick serological method of classifying strains of Rhizobium japonicum in nodules. Journal of Bacteriology 87:547
    [Google Scholar]
  13. Pankhurst C. E. 1977; Symbiotic effectiveness of antibiotic resistant mutants of fast- and slow- growing strains of Rhizobium nodulating Lotus species. Canadian Journal of Microbiology 23:1026–1033
    [Google Scholar]
  14. Pinto C. M., Phaik Y. Y., Vincent J. M. 1974; Nodulating competitiveness amongst strains of Rhizobium meliloti and R. trifolii . Australian Journal of Agricultural Research 25:317–329
    [Google Scholar]
  15. Purchase H. F., Vincent J. M. 1949; A detailed study of the field distribution of strains of clover nodule bacteria. Proceedings of the Linnean Society of New South Wales 74:227–236
    [Google Scholar]
  16. Purchase H. F., Vincent J. M., Ward L. M. 1951; Serological studies of the root nodule bacteria. Proceedings of the Linnean Society of New South Wales 76:
    [Google Scholar]
  17. Read M. P. 1953; The establishment of serologically identifiable strains of Rhizobium trifolii in field soils in competition with native microflora. Journal of General Microbiology 9:1–14
    [Google Scholar]
  18. Schwinghamer E. A., Dudman W. F. 1973; Evaluation of spectinomycin resistance as a marker for ecological studies with Rhizobium spp. Journal of Applied Bacteriology 36:263–272
    [Google Scholar]
  19. Škrdleta V. 1965; Somatic sero-groups of Rhizobium japonicum . Plant and Soil 23:43
    [Google Scholar]
  20. Vincent J. M. 1941; Serological studies of the root nodule bacteria. I. Strains of Rhizobium meliloti . Proceedings of the Linnean Society of New South Wales 66:145–154
    [Google Scholar]
  21. Vincent J. M. 1970 A Manual for the Practical Study of Root Nodule Bacteria International Biological Programme Handbook 15 Oxford: Blackwell Scientific Publications;
    [Google Scholar]
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