
Full text loading...
The intracellular distribution of phosphoenlpyruvate carboxykinase (EC 4.1.1.49) and NADP-linked malic enzyme (EC 1.1.1.40) activity in epimastigotes of Trypanosoma cruzi (Tulahuén strain) and in Crithidia fasciculata has been studied by two procedures: (i) subcellular fractionation by differential centrifugation of homogenates obtained by breaking the cells in a mortar; (ii) selective disruption of cellular membranes by digitonin treatment. Phosphoenlpyruvate carboxykinase is particulate in both organisms, as is one of the two forms of malic enzyme present in T. cruzi (malic enzyme I), whereas the other malic enzyme of T. cruzi (malic enzyme II) and the single malic enzyme of C. fasciculata are in the cytosol.
Article metrics loading...
Full text loading...
References
Data & Media loading...