SUMMARY: Cultivation of GP1 at 35°C in low PO (3·0 m) medium resulted in growth as motile curved rods (6 to 8 × 0·5 μm). However, predominantly long filaments averaging 70 to 300 μm were observed in the presence of high concentrations of PO (43·7 m) or Na (225 m) or after incubation at 25°C in low PO medium. These conditions also resulted in decreased growth rates. Tests with cell-free extracts of long and short form filaments indicated that long filament formation is probably related to a deficiency in heat-labile factor(s) required for cell separation.


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