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SUMMARY: Rhizobium leguminosarum WU163 and WU235 did not release extracellular proteins into the environment but did synthesize periplasmic proteins, including alkaline phosphatase (EC 3.1.3.1), cyclic phosphodiesterase (EC 3.1.4.d) and inorganic pyrophosphatase (EC 3.6.1.1). Fourteen periplasmic proteins, recognized by polyacrylamide gel electrophoresis, were released by lysozyme/EDTA treatment. Four of these proteins, including the alkaline phosphatase, were repressed by phosphate.
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